Malondialdehyde Adduct to Hemoglobin: A New Marker of Oxidative Stress Suitable for Full-Term and Preterm Neonates

Abstract

Oxidative stress may play a central role in the onset of many diseases during the neonatal period. Malondialdehyde (MDA) is a marker of lipid peroxidation. The aim of this study was to evaluate a new marker, the malondialdehyde adduct to hemoglobin (MDA-Hb), which is measured in red blood cells (RBCs) and thus does not require that an additional blood sample be drawn. In this prospective study, we first adapted the measurement method previously described to Hb solutions obtained from washed RBCs and then evaluated the suitability of the method for use in neonates. MDA-Hb concentrations were measured by liquid chromatography-mass spectrometry. We compared the concentrations of MDA-Hb between preterm and term neonates. Erythrocyte samples were collected at birth from 60 healthy neonates (29 full-term and 31 preterm), as well as from 50 preterm neonates with uncomplicated postnatal evolution during the first months of life. We found a significantly higher MDA-Hb concentration at birth in preterm neonates (P = 0.002). During the first months of life, MDA-Hb concentrations were 9.4 nanomol/g Hb in hospitalized preterm neonates. MDA-Hb could be used to assess oxidative stress in preterm neonates. Together with clinical variables, it could be a useful marker for oxidative stress exposition in these higher risk patients.

Adduct of Malondialdehyde to Hemoglobin: A New Marker of Oxidative Stress That Is Associated with Significant Morbidity in Preterm Infants

“…Both MDA-Hb and whole blood GSH concentrations in packed red cells were neither very low nor very high. As they were close to those measured in healthy full-term neonates at birth [18], the contribution of blood transfusions to MDA-Hb and GSH levels in preterm infants is unlikely.…”

“…Other methods, such as liquid chromatography coupled with mass spectrometry, have been proposed to improve MDA assessment [16, 17]. We recently validated a new sensitive and specific method to measure MDA adduct to hemoglobin (MDA-Hb) in neonates [18]. This method would facilitate OS evaluation over several weeks or months based on the stability of MDA-Hb, as its elimination depends on the lifespan of the erythrocyte [19].…”

Preterm birth and oxidative stress: Effects of acute physical exercise and hypoxia physiological responses

“…At birth, preterm newborns exhibit higher levels of OS markers than those born full-term ( Table 1 ). Indeed, the reported higher plasma F2-isoprostane [13] , plasma malondialdehyde (MDA) [11] , [14] , [15] , [16] , [17] , plasma MDA-hemoglobin [18] , erythrocyte membrane hydroperoxide levels [19] and plasma lipid peroxidation [20] in preterm newborns clearly show higher levels of lipid peroxidation markers. Higher blood levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) [16] , protein carbonyl [11] , [21] and desferrioxamine chelatable iron [13] show that preterm newborns also have higher levels of OS damaged DNA, proteins and erythrocytes than those born full-term.…”

“…No intrauterine growth retardation, perinatal asphyxia, infection, hemolytic disease, major malformations, difficult delivery, genetic disorder or fetal distress. ↑ 8-OHdG [13] 27/24 38.1 (37–41) 33.7 (30–36) Umbilical cord blood at birth NA ↑ plasma F2-isoprostane ↑ NPBI ↑ DCI ↑ placenta F2-isoprostane [15] 91/47 39.2 (36–42) 31.4 (23–36) Umbilical cord blood at birth 32 required assisted ventilation, 22 suffered from respiratory distress syndrome and 10 from perinatal hypoxia ↑ MDA [18] 29/31 39.4 (37.4–41.6) 31.7 (28.1–35.7) Umbilical cord blood at birth No SGA newborns. ↑ MDA adduct to hemoglobin No intensive care requirement and medical complications [25] 25/25 38.04 (36–42) 32.92 (28–35) Umbilical cord blood at birth No congenital malformations or asphyxia.…”

Study Oxidative Stress Statues In Hypertension Women

“…Malondialdehyde (MDA) [bisdiethylacetal] CHO-CH2-CHO, is a dicarbonyl, with a molecular weight of 72 Daltons, formed as a secondary product of endoperoxide [10]. MDA is an end-product of the radical-initiated oxidative decomposition of polyunsaturated fatty acids; therefore, it is frequently used as a biomarker of oxidative stress [11] 8-Hydroxydeoxyguanosine (8-OHdG) is an oxidized nucleoside which is excreted in the bodily fluids with DNA repair. 8-OHdG is a most common stable product of oxidative DNA damage after enzymatic cleavage when ROS induced 8-hydroxylation of guanine base on nuclear and mitochondrial DNA [12] and is considered a measure of DNA oxidation in response to free radicals [13].…”