Lipid Peroxidation Activity Mediated by NADPH-Cytochrome C Reductase Purified from Rabbit Liver Microsomes

Kamataki, Tetsuya; Naminohira, Shigeru; Sugita, Osamu; Kitagawa, Haruo

doi:10.1254/jjp.28.819

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…#17-283) as described previously2930. Briefly, the PVN tissue lysate was prepared by homogenization in ice-cold 1 × lysis buffer (diluted from 5 × Mg 2+ Lysis Buffer, MLB, Cat.…”

Section: Methodsmentioning

confidence: 99%

“…MDA, SOD, GSH and NAD(P)H oxidase activity in PVN were quantified using commercially available rat ELISA kits (Invitrogen Corporation, CA, USA) according to the manufacturer’s instructions29303132. NE in plasma were quantified using commercially available rat ELISA kits (Invitrogen Corporation, CA, USA) according to the manufacturer’s instructions6.…”

Section: Methodsmentioning

confidence: 99%

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Renin-angiotensin system acting on reactive oxygen species in paraventricular nucleus induces sympathetic activation via AT1R/PKCγ/Rac1 pathway in salt-induced hypertension

Huo

et al. 2017

Sci Rep

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Brain renin-angiotensin system (RAS) could regulate oxidative stress in the paraventricular nucleus (PVN) in the development of hypertension. This study was designed to explore the precise mechanisms of RAS acting on reactive oxygen species (ROS) in salt-induced hypertension. Male Wistar rats were administered with a high-salt diet (HS, 8.0% NaCl) for 8 weeks to induced hypertension. Those rats were received PVN infusion of AT1R antagonist losartan (LOS, 10 μg/h) or microinjection of small interfering RNAs for protein kinase C γ (PKCγ siRNA) once a day for 2 weeks. High salt intake resulted in higher levels of AT1R, PKCγ, Rac1 activity, superoxide and malondialdehyde (MDA) activity, but lower levels of copper/zinc superoxide dismutase (Cu/Zn-SOD), superoxide dismutase (SOD) and glutathione (GSH) in PVN than control animals. PVN infusion of LOS not only attenuated the PVN levels of AT1R, PKCγ, Rac1 activity, superoxide and decreased the arterial pressure, but also increased the PVN antioxidant capacity in hypertension. PVN microinjection of PKCγ siRNA had the same effect on LOS above responses to hypertension but no effect on PVN level of AT1R. These results, for the first time, identified that the precise signaling pathway of RAS regulating ROS in PVN is via AT1R/PKCγ/Rac1 in salt-induced hypertension.

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“…#17-283) as described previously2930. Briefly, the PVN tissue lysate was prepared by homogenization in ice-cold 1 × lysis buffer (diluted from 5 × Mg 2+ Lysis Buffer, MLB, Cat.…”

Section: Methodsmentioning

confidence: 99%