Lipid nanoparticles for brain targeting III. Long-term stability and in vivo toxicity

Blasi, Paolo; Schoubben, Aurelie Marie Madeleine; Traina, Giovanna; Manfroni, Giuseppe; Barberini, Lanfranco; Alberti, Paolo Francesco; Cirotto, Carlo; Ricci, Maurizio

doi:10.1016/j.ijpharm.2013.06.037

Cited by 47 publications

(31 citation statements)

References 28 publications

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“…In this study, the results of our cytotoxicity assays (MTT and LDH) showed that even 100 mM of SLNs alone did not cause any significant changes in cell viability and oxidative status. In parallel to our findings, recent reports indicated that SLNs could be used as safe and efficient delivery systems for brain since they had no toxicity (Blasi et al, 2013). On the contrary, in the present investigation, the application of 100 mM of SLNs-MEM induced a slight citotoxicity leading to a reduction of cell viability likely related to oxidative alterations in N2a NB and PHWB cells.…”

Section: Citotoxicity Studies Of Slns-la-memsupporting

confidence: 88%

Solid lipid nanoparticles loaded with lipoyl–memantine codrug: Preparation and characterization

Laserra

Basit

Sozio

et al. 2015

International Journal of Pharmaceutics

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Section: Citotoxicity Studies Of Slns-la-memsupporting

confidence: 88%

Solid lipid nanoparticles loaded with lipoyl–memantine codrug: Preparation and characterization

Laserra

Basit

Sozio

et al. 2015

International Journal of Pharmaceutics

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“…All batches of Nano-PSO in the previous experiments comprised droplets of 180 nm (Figure 1) since this size of nanoparticles was shown to be successful for other brain conditions. 35,36 We now compared the α-EAE activity of the 180 nm nanodrops with that of a Nano-PSO formulation in which the average diameter of the droplets is close to 30 nm, a size used for the targeting of RNA and drugs containing lipid droplets to peripheral organs, such as liver 37 or lungs. 38 To prepare these 30 nm droplets formulation, a self-emulsifying system was developed according Figure 4 Nano-PsO in the prevention and treatment of eae.…”

Section: Treatment As Compared To Prevention In Eae-induced Micementioning

confidence: 99%

Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant

Binyamin¹,

Larush²,

Frid³

et al. 2015

IJN

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Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system and is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress. In this work, we administered a nanodroplet formulation of pomegranate seed oil (PSO), denominated Nano-PSO, to mice induced for experimental autoimmune encephalomyelitis (EAE), an established model of MS. PSO comprises high levels of punicic acid, a unique polyunsaturated fatty acid considered as one of the strongest natural antioxidants. We show here that while EAE-induced mice treated with natural PSO presented some reduction in disease burden, this beneficial effect increased significantly when EAE mice were treated with Nano-PSO of specific size nanodroplets at much lower concentrations of the oil. Pathological examinations revealed that Nano-PSO administration dramatically reduced demyelination and oxidation of lipids in the brains of the affected animals, which are hallmarks of this severe neurological disease. We propose that novel formulations of natural antioxidants such as Nano-PSO may be considered for the treatment of patients suffering from demyelinating diseases. On the mechanistic side, our results demonstrate that lipid oxidation may be a seminal feature in both demyelination and neurodegeneration.

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“…Confocal fluorescence [86] studies were also applied to evaluate the uptake of brain endothelial cells. Meanwhile, acute toxicity was visualized by brain histology examination (LM & TEM) [88] and fluorescence imaging [85].…”

Section: Nervous Systemmentioning

confidence: 99%

Toxicity assessment of nanoparticles in various systems and organs

Yang

Qin

Zeng

et al. 2017

Nanotechnology Reviews

175

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Abstract:In the past decades, much attention has been paid to toxicity assessment of nanoparticles prior to clinical and biological applications. While in vitro studies have been increasing constantly, in vivo studies of nanoparticles have not established a unified system until now. Predictive models and validated standard methods are imperative. This review summarizes the current progress in approaches assessing nanotoxicity in main systems, including the hepatic and renal, gastrointestinal, pulmonary, cardiovascular, nervous, and immune systems. Histopathological studies and specific functional examinations in each system are elucidated. Related injury mechanisms are also discussed.

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Lipid nanoparticles for brain targeting III. Long-term stability and in vivo toxicity

Cited by 47 publications

References 28 publications

Solid lipid nanoparticles loaded with lipoyl–memantine codrug: Preparation and characterization

Solid lipid nanoparticles loaded with lipoyl–memantine codrug: Preparation and characterization

Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant

Toxicity assessment of nanoparticles in various systems and organs

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