Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant

Abstract

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system and is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress. In this work, we administered a nanodroplet formulation of pomegranate seed oil (PSO), denominated Nano-PSO, to mice induced for experimental autoimmune encephalomyelitis (EAE), an established model of MS. PSO comprises high levels of punicic acid, a unique polyunsaturated fatty acid considered as one of the strongest natural antioxidants. We show here that while EAE-induced mice treated with natural PSO presented some reduction in disease burden, this beneficial effect increased significantly when EAE mice were treated with Nano-PSO of specific size nanodroplets at much lower concentrations of the oil. Pathological examinations revealed that Nano-PSO administration dramatically reduced demyelination and oxidation of lipids in the brains of the affected animals, which are hallmarks of this severe neurological disease. We propose that novel formulations of natural antioxidants such as Nano-PSO may be considered for the treatment of patients suffering from demyelinating diseases. On the mechanistic side, our results demonstrate that lipid oxidation may be a seminal feature in both demyelination and neurodegeneration.

Gypenosides Prevent H2O2-Induced Retinal Ganglion Cell Apoptosis by Concurrently Suppressing the Neuronal Oxidative Stress and Inflammatory Response

“…Therefore, antioxidant treatment is considered to be a viable strategy for the treatment of inflammatory demyelinating diseases. Administration of antioxidants can reduce injury in an animal model of demyelination and MS (Binyamin et al 2015;Miller et al 2019).…”

Nanoemulsions and Their Potential Applications in Food Industry

“…GranaGard, is a submicron pomegranate seed oil emulsion, and is an innovative formulation containing natural antioxidants, Punicic acid (an Omega 5 lipid), which constitutes 80% of the nanoemuslion. The novel patented formulation was shown to delay disease onset and prevent neuronal death in animal models (Binyamin et al, 2015). Therapeutics Solutions International, Inc., has produced nutraceuticals prepared from NanoStilbene TM , stable nanoemulsion of pterostilbene (75-90 nm) from GRAS ingredients by low energy emulsification methods.…”

New Insights into the Role of Oxidative Stress Mechanisms in the Pathophysiology and Treatment of Multiple Sclerosis

“…Other studies demonstrated that the administration of Nigella sativa seeds in EAE induced in Wistar rats suppressed inflammation, enhanced remyelination in the cerebellum, and reduced the expression of transforming growth factor beta 1 (TGF β 1) [107]. In turn, a nanodroplet formulation of pomegranate seed oil (denominated nano-PSO) dramatically reduced oxidation of lipids in the brains of rats [48, 108]. …”