Lipid metabolism of acetate‐1‐¹⁴C by leukocytes from dogs fed an arteriosclerosis‐inducing diet

Abstract

Dogs were maintained on a control ration or on a semisynthetic diet, containing 5% cholesterol and 16% hydrogenated coconut oil, known to induce hyperlipemia and arteriosclerosis. Circulating leukocytes isolated from dogs fed the coconut oil containing diet were shown to incorporate 50% more radioactivity into lipids than control leukocytes when incubated with acetate-1A 4C. This increase, expressed as dpm/mg of leukocyte DNA, was not specific for any particular lipid since the distribution of radioactivity between neutral lipids and phospholipids, as well as among their subfractions, was the same regardless of the diet fed. The labeling patterns observed suggest that leukocyte fatty acid synthesis from acetate occurs predominantly, and perhaps exclusively, by the chain elongation pathway.

Effect of Phenylalanine and Its Metabolites on the Metabolism of Leucocytes and Lymphocytes

“…These cells are incapable of glyconeogenesis (Stjernholm, Noble, Dimitrov, Morton & Falor, 1969) or fatty acid synthesis de nouo (Majerus & Lastra, 1967). Glucose is incorporated into lipids predominantly as glycerol (Miras et al, 1967) whereas acetate is incorporated via mitochondria1 elongation of endogenous fatty acids (Miras, Mantzos & Levis, 1965 ;Ehrhart, Balachandran, Butkus, Lewis & Robertson, 1971). Lymphocytes incorporate acetate in the same manner (Blomstrand, 1966), though there is some evidence that phytohaemagglutinin-stimulated cells are capable of synthesis de nouo (Blomstrand & Liljeqvist, 1972).…”