Lipid metabolic pathways operating in amphibian full-grown oocytes

Alonso, Telma S.; Romanelli, Ida C. Bonini de; D’Angelo, Alessandra

doi:10.1016/0305-0491(87)90192-1

Cited by 5 publications

(7 citation statements)

References 13 publications

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“…The larger content of SM in stage IV and V oocytes with respect to stage III oocytes could be related to the above-mentioned role of this phospholipid in the maturation process that stage V oocytes may undergo. In full-grown B. arenarum and X. laevis oocytes, PC has the same abundant acyl groups (Alonso et al, 1987;Caldironi & Alonso, 1996). The PC/SM and total phospholipids/SM ratios in stage III oocytes are higher than the ratios found in stage IV and V oocytes.…”

Section: Resultsmentioning

confidence: 79%

“…Fig. In Xenopus laevis full-grown prophase-arrested oocytes, these two phospholipids also represent about 89% of the total phospholipid content (Alonso et al, 1987). In Xenopus laevis full-grown prophase-arrested oocytes, these two phospholipids also represent about 89% of the total phospholipid content (Alonso et al, 1987).…”

Section: Resultsmentioning

confidence: 90%

“…1 shows the percentage distribution of phospholipids in oocytes in stages III, IV and V. PC and PE comprise about 80% of the total amount in all analysed cases. In Xenopus laevis full-grown prophase-arrested oocytes, these two phospholipids also represent about 89% of the total phospholipid content (Alonso et al, 1987).…”

Section: Resultsmentioning

confidence: 99%

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Lipids during Bufo arenarum oogenesis

Bruzzone

Buschiazzo

Alonso

2003

Zygote

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The content and composition of phospholipids and triacylglycerols (TAGs) in Bufo arenarum oocytes in stages III and IV of their oogenesis were studied. The total amount of phospholipids in stage IV oocytes is 0.5-fold higher than in stage III oocytes. In both cases, the main phospholipids are phosphatidylcholine (PC) and phosphatidylethanolamine (PE). A striking observation concerns the high level of diphosphatidylglycerol (DPG) in stage III oocytes, which could be indicative of a relatively larger mitochondrial population with respect to other oogenetic stages. A net increase in sphingomyelin content was found during oogenesis. This fact could be related to the role of this phospholipid in the signal transductional pathways. In PC, palmitic (16:0), linoleic (18:2) and oleic (18:1) are the major fatty acids for both types of oocytes, while in PE the main acyl groups are 18:1, 16:0, arachidonic acid (20:4n6) and 18:2. PE is more unsaturated than PC and both phospholipids are more unsaturated in stage III oocytes than in stage IV oocytes. The amount of triacylglycerols is 0.3-fold higher in stage IV oocytes than in stage III oocytes. In both stages, the main fatty acids are 18:2, 18:1 and 16:0. During oogenesis, a significant increase in 18:1 and 18:3n3, and a decrease in 18:2 of TAG were found. The unsaturation index of TAGs from stage IV oocytes is higher than that from stage III oocytes. The TAG increase during oogenesis is consistent with the putative use of these lipids as a source of energy in embryo development.

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 90%

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Lipids during Bufo arenarum oogenesis

Bruzzone

Buschiazzo

Alonso

2003

Zygote

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“…Among all the individual fatty acids detected by GLC, only oleic acid, one of the major acyl components of B. arenarum oocyte PC and PE [24], increased in concentration after Dieldrin exposure (data not shown). Two different phospholipase enzymes appear to be primarily responsible for this fatty acid release [25,26].…”

Section: Discussionmentioning

confidence: 94%

The turnover of phospholipid fatty acyl chains is activated by the insecticide Dieldrin inBufo arenarum oocytes

2000

J. Biochem. Mol. Toxicol.

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“…The total phospholipid amount in mitochondria is 208.87 ± 9.68 nmol/mg protein. In both cases, PC and PE are about 80% of the total content as in full-grown oocytes of Xenopus laevis (Alonso et al, 1987). Phosphatidylserine (PS) and phosphatidic acid (PA) are minor components.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial lipids in Bufo arenarum full-grown oocytes

Gili

Alonso

2004

Zygote

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Both the content and composition of polar and neutral lipids from the mitochondrial fraction of ovarian full-grown Bufo arenarum oocytes were analysed in the present study. Triacylglycerols (TAG) represent 33% of the total lipids, followed by phosphatidylcholine (PC), free fatty acids (FFA) and phosphatidylethanolamine (PE). Diphosphatidylglycerol (DPG) or cardiolipin, a specific component of the inner mitochondrial membrane, represents about 4% of the total lipid content. Palmitic (16:0) and arachidonic (20:4n6) acids are the most abundant fatty acids in PC and PE, respectively. DPG is enriched in fatty acids with carbon chain lengths of 18, the principal component being linoleic acid. In phosphatidylinositol (PI), 20:4n6 and stearic acid (18:0) represent about 72 mol% of the total acyl group level. The main fatty acids in TAG are linoleic (18:2), oleic (18:1), and palmitic acids. The fatty acid composition of FFA and diacylglycerols (DAG) is similar, 16:0 being the most abundant acyl group. PE is the most unsaturated lipid and sphingomyelin (SM) has the lowest unsaturation index.

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Lipid metabolic pathways operating in amphibian full-grown oocytes

Cited by 5 publications

References 13 publications

Lipids during Bufo arenarum oogenesis

Lipids during Bufo arenarum oogenesis

The turnover of phospholipid fatty acyl chains is activated by the insecticide Dieldrin inBufo arenarum oocytes

Mitochondrial lipids in Bufo arenarum full-grown oocytes

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