Lipid Laden Macrophages in Respiratory Disease

Sadofsky, Laura R.; Hayman, Yvette; Hart, Simon P.; Morice, Alyn H.

doi:10.5056/jnm17036

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…However, no significant difference was seen between the two cell types. Both cell types were also equally capable of lipid accumulation, indicating cell maturity; macrophages are known to accumulate lipid in the airways [14] and diseases such as atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy)

Sadofsky

Hayman

Vance

et al. 2019

Lung

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PurposeThere is currently no true macrophage cell line and in vitro experiments requiring these cells currently require mitogenic stimulation of a macrophage precursor cell line (THP-1) or ex vivo maturation of circulating primary monocytes. In this study, we characterise a human macrophage cell line, derived from THP-1 cells, and compare its phenotype to the THP-1 cells.MethodsTHP-1 cells with and without mitogenic stimulation were compared to the newly derived macrophage-like cell line (Daisy) using microscopy, flow cytometry, phagocytosis assays, antigen binding assays and gene microarrays.ResultsWe show that the cell line grows predominantly in an adherent monolayer. A panel of antibodies were chosen to investigate the cell surface phenotype of these cells using flow cytometry. Daisy cells expressed more CD11c, CD80, CD163, CD169 and CD206, but less CD14 and CD11b compared with mitogen-stimulated THP-1 cells. Unlike stimulated THP-1 cells which were barely able to bind immune complexes, Daisy cells showed large amounts of immune complex binding. Finally, although not statistically significant, the phagocytic ability of Daisy cells was greater than mitogen-stimulated THP-1 cells, suggesting that the cell line is more similar to mature macrophages.ConclusionsThe observed phenotype suggests that Daisy cells are a good model of human macrophages with a phenotype similar to human alveolar macrophages.

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Section: Discussionmentioning

confidence: 99%

Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy)

Sadofsky

Hayman

Vance

et al. 2019

Lung

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Cytologie du lavage broncho-alvéolaire

Frankel,

Kaspi,

Roll

2024

Revue Francophone des Laboratoires

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Bronchoalveolar Lavage in a Pediatric Population

Gami

Rinaldi

Degefe

et al. 2021

American Journal of Clinical Pathology

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Objectives The cytomorphologic findings of bronchoalveolar lavage (BAL) in pediatrics were correlated with clinical symptoms. Methods Patient demographics, clinical symptoms/history, cytomorphologic findings, and oil red O (ORO) staining of 100 pediatric patients who underwent BAL between 2014 and 2016 were reviewed at a large academic institution. Results Of the 100 patients (males/females, 62:38), the most common conditions for BAL included cough (46/100), reflux (39/100), pneumonia (30/100), dysphagia (22/100), asthma (19/100), and cystic fibrosis (15/100). Sixteen of 100 patients were admitted with pulmonary symptoms from the emergency department. Cytomorphologic findings showed acute inflammation in 37 BALs and chronic inflammation in 9. Abundant thick mucin was present in 3 cytology cases from patients with cystic fibrosis. Fungal elements were detected in 3 cases (Candida, Aspergillus, and Pneumocystis jirovecii) and viral effects (rhinovirus) in one. Thirty-seven of 100 had rare ORO-positive lipid-laden macrophages (LLMs), 7 of 100 had moderate LLMs, 11 of 100 had numerous LLMs, 18 of 100 had positive staining without the degree of staining, 25 of 100 had negative ORO staining, and 2 of 100 had noncontributory ORO staining. An iron stain was done on 15 BALs (2 positive, 9 negative, and 4 noncontributory). Conclusions BAL cytology is a useful tool in pediatrics to discriminate underlying causes of aerodigestive system conditions, while ORO staining may occasionally help.

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Lipid Laden Macrophages in Respiratory Disease

Cited by 4 publications

References 6 publications

Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy)

Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy)

Cytologie du lavage broncho-alvéolaire

Bronchoalveolar Lavage in a Pediatric Population

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