Lipid hydroperoxide levels in plant tissues

Griffiths, Gareth; Leverentz, Michael K.; Silkowski, Helena; Gill, Narinder; Sánchez‐Serrano, José J.

doi:10.1093/jxb/51.349.1363

Cited by 36 publications

(64 citation statements)

References 34 publications

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“…Perhaps because of a lower sensitivity of the spectrophotometric assay, little, if any, degradation of 13-HPOT could be detected in H57 extracts, confirming that this 13-HPL constitutes a major route of 13-HPOT removal in potato leaves. Indeed, the leaves of H57 plants accumulate significantly higher levels of fatty acid hydroperoxides than wild-type plants (26). An in vivo HPL activity assay was performed with leaf discs from antisense lines.…”

Section: Antisense-mediated Depletion Of Hpl In Transgenic Potato Plamentioning

confidence: 99%

Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance

Vancanneyt

Sanz

Farmaki

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

247

189

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Hydroperoxide lyases (HPLs) catalyze the cleavage of fatty acid hydroperoxides to aldehydes and oxoacids. These volatile aldehydes play a major role in forming the aroma of many plant fruits and flowers. In addition, they have antimicrobial activity in vitro and thus are thought to be involved in the plant defense response against pest and pathogen attack. An HPL activity present in potato leaves has been characterized and shown to cleave specifically 13-hydroperoxides of both linoleic and linolenic acids to yield hexanal and 3-hexenal, respectively, and 12-oxo-dodecenoic acid. A cDNA encoding this HPL has been isolated and used to monitor gene expression in healthy and mechanically damaged potato plants. HPL gene expression is subject to developmental control, being high in young leaves and attenuated in older ones, and it is induced weakly by wounding. HPL enzymatic activity, nevertheless, remains constant in leaves of different ages and also after wounding, suggesting that posttranscriptional mechanisms may regulate its activity levels. Antisense-mediated HPL depletion in transgenic potato plants has identified this enzyme as a major route of 13-fatty acid hydroperoxide degradation in the leaves. Although these transgenic plants have highly reduced levels of both hexanal and 3-hexenal, they show no phenotypic differences compared with wild-type ones, particularly in regard to the expression of wound-induced genes. However, aphids feeding on the HPL-depleted plants display approximately a two-fold increase in fecundity above those feeding on nontransformed plants, consistent with the hypothesis that HPL-derived products have a negative impact on aphid performance. Thus, HPL-catalyzed production of C6 aldehydes may be a key step of a built-in resistance mechanism of plants against some sucking insect pests.

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Section: Antisense-mediated Depletion Of Hpl In Transgenic Potato Plamentioning

confidence: 99%

Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance

Vancanneyt

Sanz

Farmaki

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

247

189

View full text Add to dashboard Cite

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“…A tendency for such changes in membrane lipids in spring can be explained by the decomposition of phospholipids into simpler compounds, followed by the use of these compounds for the synthesis of important metabolites during plant growth initiation [20]. Sometimes, the freezing stress caused by frost desiccation leads to phospholipid degradation (peroxidation) [39,40]. Fatty acid peroxidation is significantly limited by the cell's antioxidant system, initially by low-molecular-weight compounds, mainly glutathione and ascorbate [18,40,41].…”

Section: Discussionmentioning

confidence: 99%

“…Sometimes, the freezing stress caused by frost desiccation leads to phospholipid degradation (peroxidation) [39,40]. Fatty acid peroxidation is significantly limited by the cell's antioxidant system, initially by low-molecular-weight compounds, mainly glutathione and ascorbate [18,40,41]. Changes in α-tocopherol levels have not previously been measured during the reacclimation/deacclimation of Picea needles.…”

Section: Discussionmentioning

confidence: 99%

“…Our analyses did not detect peroxidation, and no effect on the levels of free fatty acids, malondialdehyde or α-tocopherol was observed during the reacclimation/deacclimation experiment. Thus, the increase in the MP to ions was caused by changes in phospholipid content and most likely by phase changes in the membrane structure [11,39,40]. The MP to ions and water is affected by the composition and content of phospholipids (mainly PC) and their fatty acids, chiefly linolenic acid (18:3) [42,43].…”

Section: Discussionmentioning

confidence: 99%

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Differential effects of spring reacclimation and deacclimation on cell membranes of Norway spruce seedlings

Pukacki

Kamińska-Rożek

2013

Acta Soc Bot Pol

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Two-year-old seedlings of Norway spruce (Picea abies) during spring deacclimation were subjected to controlled reacclimation by exposure to low temperatures of 4/−3°C (day/night) in a cold room. The highest increase in freezing tolerance (by 7°C) was observed after 12 d of low temperature exposure, when shoot water potential (Ψ_{w shoot}) decreased to 0.64 MPa. The process of reacclimation was accompanied by an increase in the phospholipid content of needle cell membranes. This increase applied to total (PL) and individual phospholipids: phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidic acid (PA). After being exposed to the low temperature for 18 d, the seedlings were moved into the open air. This caused deacclimation, with an increase in Ψ_{w shoot} to −0.36 MPa and a decrease in the total phospholipid content and freezing tolerance of the needles. Significant correlations were observed between freezing tolerance, the membrane permeability (MP) of the needles and the phospholipid content, Ψ_{w shoot} and water content of the needles. The results show that during spring deacclimation, Norway spruce seedlings can be subjected to reacclimation, which is reflected in the phospholipid content, the biophysical changes of the membranes, and the freezing tolerance of the seedlings. During both spring deacclimation and reacclimation, water content in the needles plays a critical role in the cold tolerance of spruce seedlings

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“…Ltd, Germany). LPO level in tissues homogenate was measured by the method of Ohkawa et al 17 The LOOH activity was measured by the method of Griffiths et al 18 Activity of SOD was determined by the pyrogallol auto-oxidation inhibition assay method of Marklund et al 19 Catalase activity was estimated following the method of Aebi. 20 The GPx activity was calculated according to the method of Mohandas et al 21 Tissue glutathione content (reduced sulfhydryl groups) was estimated following the protocol of Ellman.…”

Section: Biochemical Estimationsmentioning

confidence: 99%

Effects of Pyrroloquinoline Quinone and Vitamin C on Diabetes Associated Cardiac Oxidative Damages and Hyperlipidemia in Mice: Biochemical and Histopathological Study

Kumar¹,

Kar²

2017

MOJBB

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Enhanced hyperglycemia is associated with oxidative stress and hyperlipidemia that is also responsible for an increased risk factor for diabetes associated cardiovascular problems. As pyrroloquinoline quinone (PQQ) is a known antioxidative agent, the primary aim of this study was to explore the hitherto unknown potential of PQQ in regulating STZ-induced oxidative damages in heart and associated cardiovascular problems. Healthy male mice were divided into seven groups such as normoglycemic; PQQ treated control; STZ-treated (150mg/kg); STZ+PQQ treated (5, 10 and 20mg/ kg, respectively); and STZ+Vit. C treated (50mg/kg). At the end of the experiment( after 15 days of treatment), animals were sacrificed and markers of oxidative stress, various antioxidants, lipid profiles, alterations in insulin, lactate dehydrogenase (LDH), relative risk value (RR-value), atherogenic index (AI) and cardiac tissue histology were evaluated. STZ -treated animals not only developed oxidative stress as indicated by an increase in lipid peroxidation (LPO), lipid hydroperoxide (LOOH), LDH, RR-value and AI but also by a decrease in the levels of insulin, high density lipoprotein (HDL-C), superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione. Following the administration of PQQ, particularly at 20mg/kg in diabetic animals, all these adverse effects were ameliorated with a decrease in LPO, LOOH, LDH and different lipids; with a parallel increase in cellular antioxidants, HDL-C and insulin level, suggesting cardioprotective effects of the test drug. Better effects were observed, as compared to that of Vit. C. Our study reveals that PQQ may have better ameliorating potential than Vit. C in regulation of the cardiovascular problems in diabetic patients.Keywords: diabetes mellitus, pyrroloquinoline quinine, vitamin-C, heart, lactate dehydrogenase, insulin, antioxidative agents MOJ Bioequivalence & Bioavailability Research ArticleOpen Access Effects of pyrroloquinoline quinone and vitamin C on diabetes associated cardiac oxidative damages and hyperlipidemia in mice: biochemical and histopathological study 202Copyright: ©2017 Kumar et al. studies have shown that PQQ can protect against several types of oxidative damages and irradiation injury. Recently, workers found not only the involvement of ROS in the pathogenesis of CVDs, but also suggested the use of antioxidants which play a major role in reducing the oxidative stress by scavenging the excess free radicals. 8,9 In recent years several compounds with antioxidant properties have been found to be beneficial and may protect or restore physiological function in diabetes associated CVDs, mainly plant based compounds have been investigated in details. 12 However, the recently known PQQ has not been tried with respect to its cardio-protective potential in diabetes, if any. An attempt has now been made to evaluate its role in the diabetes-induced CVDs and associated oxidative damage in heart. Citation Materials and methods AnimalsSwiss albino male mice, 7-8 weeks old...

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Lipid hydroperoxide levels in plant tissues

Cited by 36 publications

References 34 publications

Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance

Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance

Differential effects of spring reacclimation and deacclimation on cell membranes of Norway spruce seedlings

Effects of Pyrroloquinoline Quinone and Vitamin C on Diabetes Associated Cardiac Oxidative Damages and Hyperlipidemia in Mice: Biochemical and Histopathological Study

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