Lipid extractions from docosahexaenoic acid (DHA)-rich and oleaginous Chlorella sp. biomasses by organic-nanoclays

Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review

“…They concluded that acid hydrolysis prior to extraction was the simplest yet effective method to extract lipids from oleaginous microorganisms compared to bead beating, microwaving, autoclaving, or sonication. Heterogeneous solid acid catalysts have also been reported for acidic hydrolysis of biomass to improve lipid extraction [174,175]. Laurens et al (2015) reported that dilute acid pretreatment can assist lipid extraction to recover up to 97% of total fatty acids using hexane.…”

“…The Hexane extraction, 4000 rpm for 10 min, 25.6% lipid yield compared to 0.77% yield in control (total lipid content in biomass is not given) [174] Chlorella sp. 1% Organic-nanoclay, 20% based on dry biomass weight, 1 L None Hexane extraction, 6 h 750 rpm, 40-80% lipid recovery [175] Other methods Chlorella sp 2% TiO 2 for harvest and then rupture cell by UV irradiation, 365 nm, 3 h, 0.2 L 40.5 b 95% damage/disrupted by cell counting using Nile red [182] H. pluvialis Not given Induce germination of cell to weaken cell wall 1-Ethyl-3-methylimidazolium ethylsulfate, 1 min extraction, 82% astaxanthin recovery [172] H. pluvialis Single cell Crystalline gold nanoscalpel (Au-NS) to incise cells to release astaxanthin…”

Recent nanoparticle engineering advances in microalgal cultivation and harvesting processes of biodiesel production: A review

“…KR-1 could be enhanced due to the destabilization effect of algal cells as well as the increased microalgae harvesting efficiency (Lee et al, 2013d). Cationic charged aminoclay nanoparticles have played a role in weakening microalgal cell walls and decreasing the thin water layer between cells and contacting hydrophobic solvent for easy release of internal oil to outside media.…”

“…This might have been due to the unique properties of the aminoclay nanoparticles, though their effect would vary with the specific microalgal species. Aminoclay nanoparticles are composed of metal cations such as Ca 2+ , Mg 2+ and Fe 3+ at the center, sandwiched by or paired with amino functional groups lined via covalent bonding (Lee et al, 2013d). Aminoclays have abundant primary amine groups, which induce solubilization of protonated amine in aqueous solution and subsequent adsorption onto microalgal cells.…”

Reductive degradation of perfluorinated compounds in water using Mg-aminoclay coated nanoscale zero valent iron

“…Commercial air-stable nZVI powder (NANOFER STAR) was purchased from NANO IRON, s.r.o (Czech republic). MgAC was synthesized using a previously utilized method [27]. Sodium fluoride, sodium carbonate and sodium bicarbonate were obtained from Sigma Aldrich (Seelze, Germany), all with purity > 99 %.…”