Lipid droplet-associated proteins in high-fat fed mice with the effects of voluntary running and diet change

Rinnankoski‐Tuikka, Rita; Hulmi, Juha J.; Torvinen, Sira; Silvennoinen, Mika; Lehti, Maarit; Kivelä, Riikka; Reunanen, Hilkka; Kainulainen, Heikki

doi:10.1016/j.metabol.2014.05.010

Cited by 24 publications

(16 citation statements)

References 220 publications

(283 reference statements)

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“…In gastrocnemius muscle, the HFD did not increase the triglyceride content and Pparg2 expression. Different from our results, electron microscopic analysis demonstrated increased intramyocellular triglyceride contents in sedentary mice fed an HFD during 19 weeks [51]. A short-time exposure (8 weeks) to an HFD did not lead to marked lipid accumulation in skeletal muscle as also demonstrated in Swiss mice [52].…”

Section: Discussioncontrasting

confidence: 84%

DNA Methylation Changes Induced by a High-Fat Diet and Fish Oil Supplementation in the Skeletal Muscle of Mice

et al. 2014

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Background/Aims: To investigate the global changes in DNA methylation and methylation of the promoter region of the peroxisome proliferator-activated receptor gamma transcript variant 2 (Pparg2) gene resulting from a high-fat diet (HFD) and/or fish oil supplementation. Methods: Fish oil, rich in omega-3 polyunsaturated fatty acids, or water was orally administered to male mice for 12 weeks. After the first 4 weeks, the animals were fed a control diet or an HFD until the end of the experimental protocol, when the epididymal fat, gastrocnemius muscle and liver were excised. Results:Pparg2 mRNA expression was upregulated by obesity and downregulated by fish oil supplementation in the liver. In the gastrocnemius muscle, diet-induced obesity increased global DNA methylation. Fish oil prevented the decrease in Pparg2 promoter methylation induced by obesity in the gastrocnemius muscle. Regardless of the diet given, fish oil supplementation increased Pparg2 promoter methylation at CpG-263 in muscle and adipose tissue. Conclusion: HFD and fish oil modified global and Pparg2 promoter DNA methylation in a tissue-specific manner. Fish oil supplementation attenuated body weight gain, abolished the increase in Pparg2 expression in the liver and prevented the decrease in Pparg2 promoter methylation in the muscle induced by the HFD.

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Section: Discussioncontrasting

confidence: 84%

DNA Methylation Changes Induced by a High-Fat Diet and Fish Oil Supplementation in the Skeletal Muscle of Mice

et al. 2014

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“…Obesity is characterized by enhanced accumulation of FAs in adipose tissue, liver, and skeletal muscle, and HFD feeding is an accepted model for obesity and IR. Long-term HFD feeding produces an increase in LD content and PLIN5 expression in skeletal muscle [95]. As discussed above, one of the mechanisms involved in HFD-dependent IR is the presence of toxic lipid intermediates as a result of lipid management derangements.…”

Section: Findings On Insulin Resistance In Skeletal Musclementioning

confidence: 99%

Muscle Lipid Metabolism: Role of Lipid Droplets and Perilipins

Morales

Bucarey

Espinosa

2017

Journal of Diabetes Research

129

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Skeletal muscle is one of the main regulators of carbohydrate and lipid metabolism in our organism, and therefore, it is highly susceptible to changes in glucose and fatty acid (FA) availability. Skeletal muscle is an extremely complex tissue: its metabolic capacity depends on the type of fibers it is made up of and the level of stimulation it undergoes, such as acute or chronic contraction. Obesity is often associated with increased FA levels, which leads to the accumulation of toxic lipid intermediates, oxidative stress, and autophagy in skeletal fibers. This lipotoxicity is one of the most common causes of insulin resistance (IR). In this scenario, the “isolation” of certain lipids in specific cell compartments, through the action of the specific lipid droplet, perilipin (PLIN) family of proteins, is conceived as a lifeguard compensatory strategy. In this review, we summarize the cellular mechanism underlying lipid mobilization and metabolism inside skeletal muscle, focusing on the function of lipid droplets, the PLIN family of proteins, and how these entities are modified in exercise, obesity, and IR conditions.

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“…The other factor that might have contributed to these inconsistencies is the shorter dietary intervention period. Most of the previous works exposed the animals to different types of diets for longer intervention periods (15-30 weeks) [1, 2,19].…”

Section: Discussionmentioning

confidence: 99%

Comparing the metabolic fates of BALB/c mice maintained on cafeteria-style diets with differential nutritive values

Zaid

Ameer

Ali

et al. 2018

Preprint

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Cafeteria (CAF) diet-fed rodents are shown to provide a robust model of metabolic syndrome and human obesity. The carbohydrate/fat-rich food-items provided to the CAF-diet-model more closely approximate the ultra-processed human diet. However, most of the previous studies applied the commercially available rodent chow-diet for the comparative analyses and labeled it as a healthy-diet. The presented work aims to extend the knowledge on CAF-diet model by exposing the mice to human foods with different nutritional values. Our major goal was to study the metabolic fates of mice maintained on human food-items, which depending upon on their macronutrient compositions are categorized as healthy or unhealthy. BALB/c mice were randomly allocated to one of the three dietary intervention groups, standard chow diet; high-sugar/high-fat-cafeteria (HSHF-CAF) diet; or low-sugar/low-fat-cafeteria (LSLF-CAF) diet, for 5 weeks. The differences in multiple metabolic parameters (including food-/energy /macronutrient-intake, body-weight gain rate, organ-to-body weight ratios, plasma lipid profiles, adipocyte physiology, lipid deposition in metabolic tissues and ectopic fat storage in heart and kidney) were compared among the three intervention groups. We did not observe hyperphagia in mice maintained on CAF-diets. Nonetheless, the CAF-diet-fed mice displayed increased weight-gain-rate, adiposity, and adipocyte hypertrophy when compared to the chow-fed mice. However, the mice maintained on the two cafeteria-style diets displayed similar metabolic profiles, with HSHF-CAF-group displaying slightly higher weight-gain-rate. The HSHF-CAF-and LSLF-CAF-diet induced comparable adiposity in BALB/c mice. Further studies, with longer dietary intervention periods, are required to elucidate the effects of differential CAF-diets on the metabolic health of mice.

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Lipid droplet-associated proteins in high-fat fed mice with the effects of voluntary running and diet change

Cited by 24 publications

References 220 publications

DNA Methylation Changes Induced by a High-Fat Diet and Fish Oil Supplementation in the Skeletal Muscle of Mice

DNA Methylation Changes Induced by a High-Fat Diet and Fish Oil Supplementation in the Skeletal Muscle of Mice

Muscle Lipid Metabolism: Role of Lipid Droplets and Perilipins

Comparing the metabolic fates of BALB/c mice maintained on cafeteria-style diets with differential nutritive values

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