Lipid-DNA conjugates for cell membrane modification, analysis, and regulation

Bagheri, Yousef; Shafiei, Fatemeh; Chedid, Sara; Zhao, Bin; Meng, Yuchuan

doi:10.1080/10610278.2019.1632454

Cited by 14 publications

(16 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11,26,27 However, it is still not known how these variations happen and what parameters determine the membrane insertion kinetics, efficiencies, and durations of lipid-DNA probes. 28 In this study, we aim to provide an in-depth understanding of how lipid-DNA probes interact with cell membranes. With quantitative assessment using a uorescence microscope, we have determined the cell membrane insertion kinetics, magnitudes, and durations of different lipid-DNA probes.…”

Section: Introductionmentioning

confidence: 99%

A quantitative assessment of the dynamic modification of lipid–DNA probes on live cell membranes

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

A quantitative assessment of the dynamic modification of lipid–DNA probes on live cell membranes

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…So, The DNA-lipid conjugation will be immobilized on the surface of the cell membrane. [6] You competed the anchors among diacyl lipid, cholesterol and tocopherol on the Ramos cell membrane, and also calculated the membrane encounter rates among these molecules. [7] The results can provide data for further application to estimate the effective cell membrane diffusion area of each lipid.…”

Section: Non-specific Modification Methodsmentioning

confidence: 99%

Recent Progress and Perspectives on Cell Surface Modification

Zhang

Zhou

Qian

2021

Chemistry An Asian Journal

View full text Add to dashboard Cite

The cell membrane is a biological interface consisting of phospholipid bilayer, saccharides and proteins that maintains a stable metabolic intracellular environment as well as regulating and controlling the exchange of substances inside and outside the cell. Cell membranes provide a highly complex biological surface carrying a variety of essential surfaces ligands and receptors for cells to receive various stimuli of external signals, thereby inducing corresponding cell responses regulating the life activities of the cell. These surface receptors can be manipulated via cell surface modification to regulate cellular functions and behaviors Thus, cell surface modification has attracted considerable attention due to its significance in cell fate control, cell engineering and cell therapy. In this minireview, we describe the recent developments and advances of cell surface modification, and summarize the main modification methods with corresponding functions and applications. Finally, the prospect for the future development of the modification of the living cell membrane is discussed.

show abstract

“…DNA probes can be covalently modified onto target membrane lipids without affecting their lateral diffusion and domain partition (You et al, 2017 ; Bagheri et al, 2019a ). The highly precise and controllable DNA hybridizations have further allowed programmable manipulation and function of these membrane-anchored DNA probes (Bagheri et al, 2019b ; Zhao et al, 2020 ).…”

Section: Methods To Study Membrane Transient Interactionsmentioning

confidence: 99%

Current Methods for Detecting Cell Membrane Transient Interactions

2020

Self Cite

View full text Add to dashboard Cite

Short-lived cell membrane complexes play a key role in regulating cell signaling and communication. Many of these complexes are formed based on low-affinity and transient interactions among various lipids and proteins. New techniques have emerged to study these previously overlooked membrane transient interactions. Exciting functions of these transient interactions have been discovered in cellular events such as immune signaling, host–pathogen interactions, and diseases such as cancer. In this review, we have summarized current experimental methods that allow us to detect and analyze short-lived cell membrane protein–protein, lipid–protein, and lipid–lipid interactions. These methods can provide useful information about the strengths, kinetics, and/or spatial patterns of membrane transient interactions. However, each method also has its own limitations. We hope this review can be used as a guideline to help the audience to choose proper approaches for studying membrane transient interactions in different membrane trafficking and cell signaling events.

show abstract

Lipid-DNA conjugates for cell membrane modification, analysis, and regulation

Cited by 14 publications

References 92 publications

A quantitative assessment of the dynamic modification of lipid–DNA probes on live cell membranes

A quantitative assessment of the dynamic modification of lipid–DNA probes on live cell membranes

Recent Progress and Perspectives on Cell Surface Modification

Current Methods for Detecting Cell Membrane Transient Interactions

Contact Info

Product

Resources

About