Lipid Content and Composition of Oocytes from Five Coral Species: Potential Implications for Future Cryopreservation Efforts

Lin, Chiahsin; Wang, Li-Hsueh; Meng, Pei‐Jie; Chen, Chii-Shiarng; Tsai, Shu-Yao

doi:10.1371/journal.pone.0057823

Cited by 23 publications

(11 citation statements)

References 17 publications

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“…The cryopreservation of invertebrate oocytes is challenging because they are highly sensitive to the chilling temperature and have low membrane permeability (Tsai et al 2009;Lin et al , 2013Tsai et al 2016…”

Section: Oocytesmentioning

confidence: 99%

Sugars as supplemental cryoprotectants for marine organisms

Tsai

Chong

Meng

et al. 2017

Reviews in Aquaculture

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Cryopreservation has been extensively used in various mega‐industries and has recently been applied in genetic banking for conservation purposes. Compared with conventional cell preservation methods, cryopreservation can maintain the viability of cryopreserved cells for an indefinite time at a relatively lower cost and lesser manpower, with lower probabilities of contamination and genetic changes. This study presents the crucial role of sugar, a cryoprotectant supplement in cryopreservation. Sugar molecules typically interact with the lipid bilayer during the freezing phase to maintain plasma membrane integrity when cells undergo dehydration. When combined with other permeable cryoprotectants such as glucose with methanol and trehalose with dimethyl sulphoxide, sugar prolongs and enhances cellular post‐thaw viability. Moreover, terrestrial and marine organisms have benefited from the inclusion of sugar in the cryopreservation protocol. For wide range of cells such as the sperm, oocytes, embryos and larvae of marine vertebrates and invertebrates, as well as marine algae, cryopreservation with sugar produced positive results compared with cryopreservation without sugar. Not all sugar is beneficial, and the type and concentration of sugar should be applied according to the species. Moreover, the freezing method may also affect the function of sugar. Nevertheless, understanding the role of sugar in cryobiology and conducting a preliminary trial of sugar for cryopreserving cells would benefit future research on cryopreservation.

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Section: Oocytesmentioning

confidence: 99%

Sugars as supplemental cryoprotectants for marine organisms

Tsai

Chong

Meng

et al. 2017

Reviews in Aquaculture

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“…, Isa and Okazaki , Lin et al. , Wang et al. ), and can also be found as phosphate ions, polyphosphates, phosphate esters and phosphonates in cnidarians and other marine invertebrates (Kittredge and Roberts , Kittredge et al.…”

Section: Phosphorus‐containing Compounds In the Symbiotic Associationmentioning

confidence: 99%

“…The incorporation of phosphorus from various environmental sources into the different cellular compounds of the symbiotic association is still poorly understood. Overall, phosphorus is a key component of ATP (Fang et al 1991) and of phospholipids (Patton et al 1977, Isa and Okazaki 1987, Lin et al 2013, and can also be found as phosphate ions, polyphosphates, phosphate esters and phosphonates in cnidarians and other marine invertebrates (Kittredge and Roberts 1969, Quin and Quin 2001, Godinot et al 2016.…”

Section: Phosphorus-containing Compounds In the Symbiotic Associationmentioning

confidence: 99%

Phosphorus metabolism of reef organisms with algal symbionts

Ferrier‐Pagés

Godinot

D’Angelo

et al. 2016

Ecological Monographs

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Phosphorus (P), an essential structural and functional component of all living organisms, is considered to be the ultimate limiting nutrient in marine ecosystems. To optimize its acquisition, marine species such as protozoa, sponges, foraminifera, clams, and reef corals, among others, have entered symbiotic relationships with algae, which recycle waste products of the animal host and transform dissolved inorganic nutrients into organic molecules, making them bioavailable to their host. Such associations provide a competitive edge in an environment where ambient nutrient availability is low. The aim of this review is to summarize the current knowledge on the P sources available to reef organisms with algal symbionts, to discuss the means by which P is taken up and stored within the symbiosis, and to assess the effects of eutrophication‐induced phosphate enrichment on the algal and host physiology. Finally, we give an overview of knowledge gaps and open questions that should be addressed to better understand the role of phosphorus in reef symbioses functioning.

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“…Under normal circumstances there is a balance between the energy that is produced and that which is consumed (Grottoli et al, 2004), however, under stressful conditions this balance can be broken, because the energy reserves, in addition to supporting growth and reproduction, are also the source of energy to combat the consequences of the stress (Seemann et al, 2013). Lipids represent up to 40% of coral tissue dry weight and, as such, are the primary energy reserve, therefore decreases or increases in the concentrations of total lipids are useful indicators of the use or accumulation, respectively, of these reserves (Stimson, 1987;Ward, 1995;Oku et al, 2002Oku et al, , 2003Grottoli et al, 2004;Imbs, 2013;Lin et al, 2013;Conlan et al, 2017Conlan et al, , 2018. For this reason, lipid concentrations are considered to be a good indicator of coral stress.…”

Section: Introductionmentioning

confidence: 99%

Effects of Partial Mortality on Growth, Reproduction and Total Lipid Content in the Elkhorn Coral Acropora palmata

Piñón-González

Banaszak

2018

Front. Mar. Sci.

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Partial mortality (PM) is increasingly common in the Elkhorn coral Acropora palmata and, depending on the causative agent, is potentially lethal. The effects of PM on growth, reproduction and total lipid content in A. palmata were studied by sampling apparently healthy (AH) colonies in comparison with colonies showing signs of PM. Branch growth rates and lesion regeneration rates were estimated using monthly photographs over a four-month period prior to the summer spawning season. No differences were found in the growth rates of colonies with PM compared to AH colonies. The areas affected by PM did not regenerate during the period of the study. Colonization of the lesions by competing species and sediment cover were documented and did not show major changes. During the spawning season, percent fertilization, egg volume and embryonic development were evaluated for comparison between AH colonies and those with PM. Total lipids were also quantified in tissues from three branches per colony. Percentage fertilization was similar in both AH colonies and those with PM. Embryonic development was normal, regardless of proximity to the lesion borders. However, egg volume was significantly lower in PM colonies than in AH colonies. Lower lipid concentrations were found at the edges of the lesions and similar to those found at the growing edges of the branches. The lack of regeneration may be explained by the low lipid concentration, because the polyps adjacent to the lesion do not have an adequate energy budget as a result of the damage. This would also affect their ability to compete against organisms that colonize the site of the lesion, a distinct situation to the rapid regeneration rates characteristic of lesions due to physical injury of the colony. Therefore, we conclude that partial mortality in A. palmata affects the colony, inducing energetic stress due to both competition and decreased egg quality.

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Lipid Content and Composition of Oocytes from Five Coral Species: Potential Implications for Future Cryopreservation Efforts

Cited by 23 publications

References 17 publications

Sugars as supplemental cryoprotectants for marine organisms

Sugars as supplemental cryoprotectants for marine organisms

Phosphorus metabolism of reef organisms with algal symbionts

Effects of Partial Mortality on Growth, Reproduction and Total Lipid Content in the Elkhorn Coral Acropora palmata

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