Lipid Composition of the Filarial Parasite Setaria cervi

Ansari, Afzal A.; Yusufi, A. N. K.; Siddiqi, Majid

doi:10.2307/3278446

Cited by 15 publications

(8 citation statements)

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“…Microfilariae contain more lipids, nucleic acids, mucopolysaccharides and total phosphorus than adults. All the major classes of lipids have been demonstrated in S. cervi adults (Ansari et al, 1973) as well as mf (Rathaur et al, 1980). The phospholipid percentage is higher in mf than in adults.…”

Section: Biochemical Compositionmentioning

confidence: 93%

“…All the major classes of lipids have been demonstrated in S . cervi adults (Ansari et al , 1973) as well as mf (Rathaur et al , 1980). The phospholipid percentage is higher in mf than in adults.…”

Section: Biochemical Compositionmentioning

confidence: 99%

“…Analysis of lipid composition has been done in S . cervi and was found to be in the range from 1.3 to 8% (Ansari et al , 1973; Khatoon et al , 1984). Triacylglycerols and sterols have been demonstrated in adult S .…”

Section: Lipid Metabolismmentioning

confidence: 99%

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Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Ahmad

Srivastava

2007

J. Helminthol.

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The main problem regarding the chemotherapy of filariasis is that no safe and effective drug is available yet to combat the adult human filarial worms. Setaria cervi, the causal organism of setariasis and lumbar paralysis in cattle, is routinely employed as a model organism for conducting biochemical and enzymatic studies on filarial parasites. In view of the practical difficulties in procuring human strains of Wuchereria bancrofti and Brugia malayi for drug screening, the bovine filarial parasite S. cervi, resembling the human species in having microfilarial periodicity and chemotherapeutic response to known antifilarial agents, is widely used as a model in such studies. For a rational approach to antifilarial chemotherapy, knowledge of the biochemical composition and metabolic pathways of this helminth parasite may be of paramount importance, so that more potent antifilarial agents based on specific drug targets can be identified in drug discovery programmes. The present review provides an update on the biochemistry of the important metabolic pathways functioning within this potentially important bovine parasite, that have so far been studied, and on those that need to be investigated further so as to identify novel drug targets that can be exploited for designing new antifilarial drugs.

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Section: Biochemical Compositionmentioning

confidence: 93%

Section: Biochemical Compositionmentioning

confidence: 99%

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Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Ahmad

Srivastava

2007

J. Helminthol.

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“…It is known that the worms readily incorporate labelled lipid precursors into their neutral and phospholipid fractions in their lipid bio-synthesis [14,15,16]. The entire major classes of neutral lipids namely, triacylglycerols, diacylglycerols sterols, sterol esters, hydrocarbons and traces of free fatty acids have been verified in Dirofilaria immitis [13,16,17,18] while triacylglycerols and sterols have been demonstrated in adult Setaria cervi [19,20].The adult Brugia pahangi has been reported to have triacylglycerols, sterols, as well as free fatty acids and hydrocarbon fractions in their tissues [18]. All the major phospholipid classes have also been identified in adult filarial worms of D. immitis, B. pahangi, Litomosoides carinii and S. cervi [13,19,21,22].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Study on lipid Catabolism in Adult Onchocerca Volvulus

Abdulai¹

2014

AJBLS

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Onchocerca volvulus is a parasite responsible for the disease, Onchocerciasis otherwise known as river blindness. Current treatment and control strategies are not entirely successful. This means there is a need for further studies to better understand the biology of this worm. Studies on metabolic pathways in the worm can provide valuable information to deepen our understanding of the mechanism of survival of the worm. Lipid catabolism for the provision of energy in this worm still remains to be elucidated. Knowledge of lipids composition and synthesis in this parasite has been mostly drawn from studies on filarial species other than O. volvulus itself. This is owed to the limited availability of parasite material due largely to problems with parasite cultivation and ethical demands on obtaining materials since it has a strict preference for the human host. To explore lipid catabolism in the worm itself, we performed immunohistochemical localisation of three major enzymes (A/B hydrolase, SDH and ME1) involved in lipid metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 58.6% of worms in the paraffin processed nodules had detectable A/B/HD4, 51.6% had SDH and 63.3% had ME 1, most of which were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the B-oxidative pathway, The TCA cycle, and undergo anaplerotic transformation of malic acid to pyruvate to maximise energy production from lipid metabolism. This also suggests that O. volvulus has the capacity to catabolise lipids for energy even though it appears to be dependent on carbohydrate metabolism.

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“…Development of suitable chemotherapeutic agents for effective control of parasites depends on our understanding of the composition of biochemical entities and metabolism of the latter and also the differences with the host tissues, if any. However, such information for parasitic nematodes is limited, although certain aspects of lipid metabolism have been worked out for other nematodes, such as Dirofilaria immitis (HUTCHISON et al, 1976), Ascaris lumbricoides (BARRETT et al, 1970), Caenorhabditis briggsae and Panagrellus redivivus (ROTHSTEIN, 1970), Setaria cervi (ANSARI et al, 1973), Litomosoides carinii (SUBRAHAMANYAM, 1967) and Haemonchus contortus (KAPOOR & SOOD, 1987). In most of these studies, however, data on qualitative lipid analysis of the parasite is presented without providing sufficient insight into the dynamics of lipid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Lipid metabolism inTrichuris globulosa(Nematoda)

1989

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Adult males and females ofTrichuris globulosa, an intestinal nematode parasite of goats, were studied for their lipid composition, capability of incorporation of (Na)-1-14C-acetate into different lipid classes and the activity of certain key enzymes of lipid metabolism. The parasite possesses a large variety of lipids including certain complex lipids. These are phosphatidylcholine (PC), diphosphatidylglycerol (cardiolipin), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), phosphatidylserine (PS), phosphatidylinositol (PI), plasmalogens (choline+ethanolamine), mono-, di- and triacylglycerols, free and esterified cholesterol, non-esterified fatty acids (NEFA), gangliosides, cerebrosides (glycosyl ceramide) and sulphuric acid esters of cerebrosides (sulphatides). The females contain more lipids than males, particularly the acylglycerols and phospholipids, possibly to meet the energy requirement and structural entities for the daily production of large numbers of eggs. Incorporation studies of labelled substrate, sodium-1-14C acetate demonstrate that the adult female has extremely active mechanisms for biosynthesizing these lipids. Most of the labels are found in PC, PE, SM, acylglycerols, NEFA, gangliosides, cerebrosides and sulphatides. Cholesterol, although a minor component of the parasitic lipids, incorporates large amount of label and also undergoes fast turnover. Kinetic analysis of the incorporation by measuring the rate constant (k) and half life (t½) reveals that gangliosides are the fastest biosynthesizing and turning over lipids, although they constitute only 0·1% of the total lipids. The presence of important enzymes of lipid biosynthesis, glucose-6-phosphate dehydrogenase, malate dehydrogenase and hydroxymethyl glutaryl-CoA reductase and an enzyme of lipid ester hydrolysis, triacylglycerol lipase, is also established inT. globulosa. Michaelis-Menten kinetic characteristics of the parasitic enzymes (Km, Vmax, v and the first order rate constant, k) are comparable with those of rat liver homogenates.

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Lipid Composition of the Filarial Parasite Setaria cervi

Cited by 15 publications

References 0 publications

Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Biochemical composition and metabolic pathways of filarial worms Setaria cervi: search for new antifilarial agents

Immunohistochemical Study on lipid Catabolism in Adult Onchocerca Volvulus

Lipid metabolism inTrichuris globulosa(Nematoda)

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