Lipid composition of the canine sperm plasma membrane as markers of sperm motility

Lúcio, Cristina de Fátima; Brito, Maíra Morales; Angrimani, Daniel de Souza Ramos; Belaz, K. R. A.; Morais, Damila Rodrigues de; Zampieri, Dávila; Losano, João Diego de Agostini; Assumpção, M. E. O. A.; Nichi, Marcílio; Eberlin, Marcos N.; Vannucchi, Camila Infantosi

doi:10.1111/rda.12860

Cited by 33 publications

(32 citation statements)

References 26 publications

(35 reference statements)

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“…Since the lipid composition of the sperm plasma membrane is a major determinant of sperm motility [ 91 ], we expected to observe changes in sperm motility with the incorporation of exogenous GPL. To test this hypothesis and get functional data on the incorporation of GPL from the sub-μm-sized micelles into sperm membranes, we measured the motility of human sperm with Computer Assisted Sperm Analysis (CASA) after incubation of various concentrations of sub-μm-sized micelles made with different concentrations of GPL.…”

Section: Resultsmentioning

confidence: 99%

Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress

et al. 2018

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Membrane integrity is essential in maintaining sperm viability, signaling, and motility, which are essential for fertilization. Sperm are highly susceptible to oxidative stress, as they are rich in sensitive polyunsaturated fatty acids (PUFA), and are unable to synthesize and repair many essential membrane constituents. Because of this, sperm cellular membranes are important targets of this process. Membrane Lipid Replacement (MLR) with glycerophospholipid mixtures (GPL) has been shown to ameliorate oxidative stress in cells, restore their cellular membranes, and prevent loss of function. Therefore, we tested the effects of MLR on sperm by tracking and monitoring GPL incorporation into their membrane systems and studying their effects on sperm motility and viability under different experimental conditions. Incubation of sperm with mixtures of exogenous, unoxidized GPL results in their incorporation into sperm membranes, as shown by the use of fluorescent dyes attached to GPL. The percent overall (total) sperm motility was increased from 52±2.5% to 68±1.34% after adding GPL to the incubation media, and overall sperm motility was recovered from 7±2% after H2O2 treatment to 58±2.5%)(n = 8, p<0.01) by the incorporation of GPL into sperm membranes. When sperm were exposed to H2O2, the mitochondrial inner membrane potential (MIMP), monitored using the MIMP tracker dye JC-1 in flow cytometry, diminished, whereas the addition of GPL prevented the decrease in MIMP. Confocal microscopy with Rhodamine-123 and JC-1 confirmed the mitochondrial localization of the dyes. We conclude that incubation of human sperm with glycerolphospholipids into the membranes of sperm improves sperm viability, motility, and resistance to oxidizing agents like H2O2. This suggests that human sperm might be useful to test innovative new treatments like MLR, since such treatments could improve fertility when it is adversely affected by increased oxidative stress.

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Section: Resultsmentioning

confidence: 99%

Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress

et al. 2018

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“…The lipid composition of the plasma membrane (cholesterol and unsaturated fatty acids) is directly related to the motility of the sperm and the fluidity of the membrane; the presence of unsaturated fatty acids gives the membrane greater fluidity. Lucio et al (2017) have reported the presence of both saturated (palmitic and stearic) and unsaturated fatty acids (arachidonic and oleic) in dog spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

The effect of non-traditional cooling on dog sperm cryosurvival and ability to perform the acrosome reaction

Ortega-Morales¹,

Alcantar-Rodriguez²,

Espejel³

et al. 2019

Austral j. vet. sci.

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The objective of this study was to assess cryosurvival, plasma membrane fluidity, and capability of cryopreserved dog (Canis lupus familiaris) spermatozoa, cooled to -5 °C before freezing, to perform the acrosome reaction under the effect of progesterone and calcium ionophore. In the first experiment, fresh spermatozoa diluted in Tyrode's medium plus albumin, lactate, and pyruvate (TALP) were incubated at 38 °C in 5% CO 2 in air, with progesterone or calcium ionophore added at 2, 4, and 6 h after incubation and sampled 30 min later to assess the acrosome reaction. In the second experiment, diluted sperm were packaged in plastic straws, cooled to either +5 °C or -5 °C and cryopreserved. Progressive motility, plasma membrane integrity and fluidity, capacitation status and acrosome integrity were assessed before and after freeze-thawing. After thawing, sperm were assessed, resuspended in TALP and incubated to assess the acrosome reaction. Parameters for sperm cryosurvival were similar in sperm cooled to either +5 °C or -5 °C, except in the percentage of hyper-fluid membranes which was lower (P<0.05) in sperm cooled to -5 °C. There were no differences in the percentages of frozen-thawed spermatozoa with acrosome reaction, induced by progesterone or calcium ionophore, between cooling treatments. In conclusion, cooling of dog spermatozoa to -5 °C did not improve sperm cryosurvival but had a positive effect on plasma membrane fluidity.

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“…Cholesterol stiffens the sperm plasma membrane and makes it more permeable for different molecules and compounds. Unfortunately, the presence of double bonds increases the chemical reactivity, which may lead to the loss of motility and membrane damage (Lucio et al, ). Lucio et al () have shown that lipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylcholine plasmanyl, phosphatidylcholine plasmenyl and phosphatidic acid might be treated as markers of sperm motility.…”

Section: Structure Of Spermatozoamentioning

confidence: 99%

“…Unfortunately, the presence of double bonds increases the chemical reactivity, which may lead to the loss of motility and membrane damage . Lucio et al (2017) have shown that lipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylcholine plasmanyl, phosphatidylcholine plasmenyl and phosphatidic acid might be treated as markers of sperm motility. Different membrane proteins are localized in the plasma membrane of spermatozoa.…”

Section: Matur Ati On Of S Permatozoa In the Epid Idymismentioning

confidence: 99%

Canine spermatozoa—What do we know about their morphology and physiology? An overview

Chłopik

Wysokińska

2019

Reprod Domestic Animals

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Spermatozoa are unique cells because of their morphological and physiological characteristics. They are produced during the process called spermatogenesis. Spermatogenesis consists of three phases: spermatocytogenesis, spermiogenesis and spermiation, during which spermatozoa undergo several changes. Spermatogenesis takes place within the seminiferous tubules containing two types of cells—the germ cells and the Sertoli cells—that alongside the Leydig cells, which play an important role when it comes to normal fertility. Everything is regulated by the hypothalamic–pituitary–gonadal axis and specific hormones due to multi‐hormonal feedback systems. Spermatozoa possess morphological and physiological features, which are sometimes completely different from what is observed in various somatic cells. What is more, canine spermatozoa have specific characteristics making them special compared to the spermatozoa of other mammalian species. The metabolic energy production, which is crucial for the appropriate functioning of spermatozoa, can be fuelled by different metabolic pathways utilizing different chemical substrates. Inseparable from the oxidative phosphorylation process is the production of reactive oxygen species, which are both essential and toxic to spermatozoa. Furthermore, epididymis is a very important structure, responsible for the transport and maturation of spermatozoa, which are then stored in the last segment of epididymis—the epididymal cauda. Moreover, the retrieval of spermatozoa from the epididymides is crucial for the development of assisted reproduction techniques and sperm cryopreservation methods. The information gained from the research on domestic dogs might be transferred to their wild relatives, especially those species categorized as endangered.

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Lipid composition of the canine sperm plasma membrane as markers of sperm motility

Cited by 33 publications

References 26 publications

Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress

Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress

The effect of non-traditional cooling on dog sperm cryosurvival and ability to perform the acrosome reaction

Canine spermatozoa—What do we know about their morphology and physiology? An overview

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