Lipid and Wall Amino Acid Composition in the Classification of Rothia dentocariosa

Embley, T. Martin; Goodfellow, M.; Minnikin, David E.; O'Donnell, Anthony G.

doi:10.1016/s0174-3031(84)80054-2

Cited by 6 publications

(7 citation statements)

References 42 publications

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“…We surveyed the literature for information about the fatty acid content of microbial strains from the genera Acinetobacter, Mycobacterium, Veillonella, Ralstonia, and Rothia. Strains of the genus Rothia can contain high abundances of anteiso fatty acids (30). Rothia was not present (in amounts detectable by NanoString) in the patients participating in this study.…”

Section: Resultsmentioning

confidence: 76%

Refining the Application of Microbial Lipids as Tracers of Staphylococcus aureus Growth Rates in Cystic Fibrosis Sputum

et al. 2018

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Chronic lung infections in cystic fibrosis (CF) could be treated more effectively if the effects of antimicrobials on pathogens in situ were known. Here, we compared changes in the microbial community composition and pathogen growth rates in longitudinal studies of seven pediatric CF patients undergoing intravenous antibiotic administration during pulmonary exacerbations. The microbial community composition was determined by counting rRNA with NanoString DNA analysis, and growth rates were obtained by incubating CF sputum with heavy water and tracing incorporation of deuterium into two branched-chain (“anteiso”) fatty acids (a-C15:0 and a-C17:0) using gas chromatography-mass spectrometry (GC/MS). Prior to this study, both lipids were thought to be specific for Staphylococcaceae; hence, their isotopic enrichment was interpreted as a growth proxy for Staphylococcus aureus. Our experiments revealed, however, that Prevotella is also a relevant microbial producer of a-C17:0 fatty acid in some CF patients; thus, deuterium incorporation into these lipids is better interpreted as a more general pathogen growth rate proxy. Even accounting for a small nonmicrobial background source detected in some patient samples, a-C15:0 fatty acid still appears to be a relatively robust proxy for CF pathogens, revealing a median generation time of ∼1.5 days, similar to prior observations. Contrary to our expectation, pathogen growth rates remained relatively stable throughout exacerbation treatment. We suggest two straightforward “best practices” for application of stable-isotope probing to CF sputum metabolites: (i) parallel determination of microbial community composition in CF sputum using culture-independent tools and (ii) assessing background levels of the diagnostic metabolite. IMPORTANCE In chronic lung infections, populations of microbial pathogens change and mature in ways that are often unknown, which makes it challenging to identify appropriate treatment options. A promising tool to better understand the physiology of microorganisms in a patient is stable-isotope probing, which we previously developed to estimate the growth rates of S. aureus in cystic fibrosis (CF) sputum. Here, we tracked microbial communities in a cohort of CF patients and found that anteiso fatty acids can also originate from other sources in CF sputum. This awareness led us to develop a new workflow for the application of stable-isotope probing in this context, improving our ability to estimate pathogen generation times in clinical samples.

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Section: Resultsmentioning

confidence: 76%

Refining the Application of Microbial Lipids as Tracers of Staphylococcus aureus Growth Rates in Cystic Fibrosis Sputum

et al. 2018

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“…Amino acids l -Orn, d -Orn, l -Asp, d -Asp, l -Glu, d -Glu, l -Ala, d -Ala and Gly were purchased from Sigma as standards. Polar lipids were extracted and identified by two-dimensional TLC by using the method of Embley et al [30]. Menaquinones were extracted, purified by the method of Collins et al [31] and analysed by reversed-phase HPLC [32].…”

Section: Full-textmentioning

confidence: 99%

Ornithinimicrobium cerasi sp. nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium

Fang

Bai

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.) G. Don, was taxonomically characterized based on a polyphasic investigation. It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species. The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium , but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species. The menaquinone was MK-8(H4). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs). The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0. The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp. The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp. nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain. The DNA G+C composition is 72.3 mol%. The availability of new data allows for an emended description of the genus Ornithinimicrobium .

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“…The polar lipids are di‐ and mono‐phosphatidylglycerol, and the menaquinones are unsaturated with seven isoprene units. The cell‐wall sugars are galactose, fructose, glucose and ribose [17–19]. The bacterium is fermentative, with the main end‐products of carbohydrate metabolism being lactic and acetic acids.…”

Section: Characteristics Of R Dentocariosamentioning

confidence: 99%

Rothia dentocariosa: taxonomy and differential diagnosis

Graevenitz¹

2004

Clinical Microbiology and Infection

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Lipid and Wall Amino Acid Composition in the Classification of Rothia dentocariosa

Cited by 6 publications

References 42 publications

Refining the Application of Microbial Lipids as Tracers of Staphylococcus aureus Growth Rates in Cystic Fibrosis Sputum

Refining the Application of Microbial Lipids as Tracers of Staphylococcus aureus Growth Rates in Cystic Fibrosis Sputum

Ornithinimicrobium cerasi sp. nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium

Rothia dentocariosa: taxonomy and differential diagnosis

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