Lipid and salt effects on carbocyanine dye-induced photo-voltages in bilayer membranes

Baker, Johnny A.; Duchek, John R.; Hooper, Rebecca L.; Koftan, Ryan J.; Huebner, Jay S.

doi:10.1016/0005-2736(79)90026-9

Cited by 10 publications

(9 citation statements)

References 28 publications

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“…Photo-voltages are induced across bilayer membranes by a number of electrically charged cyanine dyes (4,5), probably by physical movements of dye in the membrane's surface layer after photo-isomerization (5,6). Bis-Q has two stable isomeric forms (1-3), illustrated in Fig.…”

Section: Introductionmentioning

confidence: 99%

Voltage transients from photo-isomerizing azo dye in bilayer membranes

Duchek¹,

Huebner²

1979

Biophysical Journal

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Voltage transients are induced by brief light flashed on bilayer membranes with absorbed 3,3'-bis(alpha-(trimethylammonium)methyl)azobenzene (Bis-Q). The voltages are positive for trans-to-cis photo-isomerization, and negative for cis-to-trans photo-isomerization. The risetimes in phosphatidylethanolamine-decane bilayer membranes indicate that absorbed trans-Bis-Q is photo-isomerized to cis within 2 microseconds, and that cis is photo-isomerized to trans within 15 microseconds.

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Section: Introductionmentioning

confidence: 99%

Voltage transients from photo-isomerizing azo dye in bilayer membranes

Duchek¹,

Huebner²

1979

Biophysical Journal

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“…2). Reduced erythrosin sorption to the membrane may also occur with high salt concentrations, as has been observed for a carbocyanine dye (2).…”

Section: Discussionmentioning

confidence: 93%

“…The displacement of divalent azo and monovalent carbocyanine dye following photo-isomerization result in voltage transients with rise times in the 2 to 20 gsec [7] and 0.3 to 3 msec [2] ranges, respectively. Erythrosin is of comparable size.…”

Section: Discussionmentioning

confidence: 99%

“…The membrane thickness, as defined by capacitance [9,19] and photo-effect measurements [2,7], is approximately that of the hydrocarbon membrane core plus a Debye length on each side. Charge movements occurring more than a Debye length from the core are effectively in the bulk aqueous solution and thus do not alter the membrane voltage [2,7]. The fact that the fast part was observed with 5 M NaC1 solutions (Debye length ~ 1 A), yet sorbed dye was exposed to the pH of the adjacent aqueous solutions suggests dye was in the glycerol regions of the membranes.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in acidity may also be expected for triplet states. Photo-voltages resulting from transverse displacement of electrons and ions in membranes, including displacements which cross only part of the membrane, have been considered by a number of investigators [2,5,7,13,14,34,40]. Making assumption that are not restrictive here [2], the voltage variations expected from such charge displacements in a membrane are V (t)=n e x (t)/Cm (1) where n is the number of charges e that are displaced transversely in the membrane by x(t), where x(t) is measured as the distance the charge moves divided by the membrane thickness.…”

Section: Introductionmentioning

confidence: 99%

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Erythrosin and pH gradient induced photo-voltages in bilayer membranes

et al. 1982

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Erythrosin and light flashes induce voltage transients across bilayer membranes in the presence of transmembrane pH gradients. Fast voltage transients, which rise in less than 50 nsec and fall in approximately 500 nsec, are attributed to photo-deprotonation of dye sorbed in the glycerol region of phospholipid membranes. Six other halogenated xanthene dyes induce similar effects, which apparently resulted from triplet states of monoanionic dye. No photo-effects were observed with fluorescein.

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