Effect of lipid composition changes on carbocyanine dye fluorescent response

Lipid vesicle composition influences the incorporation and fluorescence properties of the lipophilic sulphonated carbocyanine dye SP-DiO

“…[6][7][8][9][10][11][12][13][14] The photophysical properties of lipophilic membrane dyes are often greatly affected by the chemical environment and the surrounding lipids. [15][16][17] Moreover, the exact lipid composition of the membrane, including its charge and fluidity, can strongly influence the insertion efficiency of the dye and hence its effective concentration in a particular lipid vesicle. [17][18][19] As a consequence, fluorescence intensities may vary greatly between samples with the same vesicle concentration and direct quantitative comparison of fluorescence data measured in different lipid systems becomes cumbersome.…”

The characterization of Lucilia cuprina acetylcholinesterase as a drug target, and the identification of novel inhibitors by high throughput screening

“…This type of compound somewhat resembles thioflavin T (61), the anthelmintic product, dithiazanine (Aguilar, 1959), and a series of other anthelmintically active compounds (Garmaise et al, 1967(Garmaise et al, , 1969. However, it also resembles carbocyanine dyes that are known to interact strongly with a variety of biological structures and macromolecules, such as lipid membranes, glycoconjugates, proteins and nucleic acids (Green, 1978;Deleers et al, 1984). Carbocyanine dyes are also known to be potent DNA-intercalating agents (Biver et al, 2005).…”