Lipid accumulation in hepatocytes following treatment in vitro with a venom sac extract of the Oriental hornet (Vespa orientalis)

Barr-Nea, L.; Papo, Naomi; Ishay, Jacob S.

doi:10.1016/0041-0101(79)90299-x

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…General symptoms of dyspnea, bronchospasm, lower nephron damage with hemoglobinuria were described by Somerville (1975) and by Chugh et al (1976). Various morphologic liver injuries due to stings were described by Wegelin (1948), Gordon (1950), Schenken et al (1953, Glaser (1956), Wey (1956), Jonas & Sugar (1963), O'Connor et al (1963), Borochowitz & Hardoff (1982), Leclercq et al (1985), Weitzman et al (1985) and experimentally by Barr-Nea et al (1979). The lesions consisted of congestion, hemorrhage, steatosis, centrolobular necrosis, cholestasis, and Reye's syndrome-like picture.…”

Section: Vespa Orientalismentioning

confidence: 96%

“…The venom apparatus of K orientalis was studied and described by Kanwar & Sethi (1971), Kanwar & Kanwar (1975) and Barr-Nea et al (1976). It is comprised of 2 acid glands, 1 alkaline gland, a venom sac and the stinger.…”

Section: Vespa Orientalismentioning

confidence: 99%

“…Methods for collecting the venom from living or dead Vespinae have been described in the literature and include the use of high voltage shock (O'Connor et al 1963; Eskridge et al 1981), nest collection with "milking" device (Simon & Benton 1969), anesthetizing and freezing the entire colony (Ishay 1964;Edery et al 1972;Ishay 1975) or extraction of the acid and alkaline glands from dead insects (Jaques & Schachter 1954; Said 1960; Barr-Nea et al 1976). Ishay (1964), Edery et al (1972) and Ishay (1975) described the method used by us (see Materials and Methods).…”

Section: Vespa Orientalismentioning

confidence: 99%

“…These reports suggest that the venom has an adverse effect on the liver but secondary effects of shock cannot be ruled out. It has been noted that VSE has a specific effect on liver tissue culture (Barr-Nea et al 1979). The authors showed increased lipid accumulation in cells of embryonic chick liver culture, after addition of VSE to the medium, by an unidentified mechanism, noting no change in liver cell membrane permeability or intracellular metabolism.…”

Section: Hepato Toxicitymentioning

confidence: 99%

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Hepatotoxicity Induced by the Oriental Hornet (Vespa orientalis) Venom Sac Extract

Neuman

Ishay

Waron

et al. 1991

Pharmacology & Toxicology

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Experiments were performed to investigate the nature of the in vitro and human liver damage exposed to hornets' acute or repeated stings. The hornet investigated is the one ubiquitous in Israel - Vespa orientalis. Experiments were performed in living cats and rats, after single or multiple exposures to venom-sac extracts (VSE) and in various doses. The injury was demonstrated by the increased levels of enzymes, bile acids and cholesterol in serum. Also measured was Beta-N-acetyl hexosaminidase (BNAH) which probably is the only biochemical indicator available of Kupffer-cell function. This, too, was found increased. Other experiments consisted of perfusion of the isolated, intact, rat liver in situ with measurements of enzyme leakage into perfusate and of bile flow. Another set of experiments involved the effects of VSE on in vitro monolayer tissue culture of rat embryos' livers. We examined damage to organelles and compared the damage produced by intact VSE with that produced by the venom sac extract after treatment by heat or dialysis. Light morphology, special stains, electron microscopy and morphometry were all performed. In the first set of experiments no shock was observed in cats and rats exposed to VSE. The increases in enzymes' activity in serum and liver perfusion fluid were significant. Histochemistry indicated decrease of hepatic glycogen and of cellular succinic dehydrogenase as well as hepatic fat infiltration and an increase of alkaline phosphatase activity in liver cells close to the bile capillaries.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Vespa Orientalismentioning

confidence: 96%

Section: Vespa Orientalismentioning

confidence: 99%

Section: Vespa Orientalismentioning

confidence: 99%

Section: Hepato Toxicitymentioning

confidence: 99%

See 2 more Smart Citations

Hepatotoxicity Induced by the Oriental Hornet (Vespa orientalis) Venom Sac Extract

Neuman

Ishay

Waron

et al. 1991

Pharmacology & Toxicology

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“…The discrepancy might be due to the very large amount of venom that the baby received, or to a different sensitivity of adult rat liver and human baby liver. When chick hepatocytes are cultivated in direct contact with hornet venom a marked lipid infiltration is observed in the cells (14).…”

Section: Discussionmentioning

confidence: 99%

Histopathological changes in rat liver following repeated vespine envenomation

Barr-Nea¹,

Neuman

Eschar

et al. 1985

Liver

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ABSTRACT— The histopathological picture of rat liver was studied after repeated envenomation with hornet (Vespa orientalis) venom sac extract (VSE). Light microscopical and histochemical investigation showed foci of necrosis of the hepatocytes, a decrease in glycogen content and in succinic dehydrogenase activity, some fat infiltration and an increase in alkaline phosphatase activity. Electron microscopy showed a decrease in the number of mitochondria per cell as well as a decrease in their cristae, enlargement of bile canaliculi, and destruction of the endothelium of the sinusoids bringing the cytoplasm of the necrotic hepatocytes in contact with the lumen of the sinusoid. The rat liver is proposed as a model for the study of pathological effects of animal toxins.

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