Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosaunder three nutrition stressors

Fan, Jianhua; Cui, Yanbin; Wan, Minxi; Wang, Weiliang; Li, Yuanguang

doi:10.1186/1754-6834-7-17

Cited by 270 publications

(134 citation statements)

References 54 publications

(65 reference statements)

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“…Collectively, these findings reveal the complex interactions of the central metabolic pathways that jointly support the switch. Msanne et al, 2012;Fan et al, 2014aFan et al, , 2014bSchmollinger et al, 2014). Most of the photosystem proteins, light-harvesting complexes, and the cytochrome b 6 f complex were not down-regulated until 72 h in darkness (Supplemental Data Set S3).…”

Section: Dynamics Of the Central Metabolism That Supports The Switchmentioning

confidence: 99%

Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.

et al. 2015

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The ability to rapidly switch the intracellular energy storage form from starch to lipids is an advantageous trait for microalgae feedstock. To probe this mechanism, we sequenced the 56.8-Mbp genome of Chlorella pyrenoidosa FACHB-9, an industrial production strain for protein, starch, and lipids. The genome exhibits positive selection and gene family expansion in lipid and carbohydrate metabolism and genes related to cell cycle and stress response. Moreover, 10 lipid metabolism genes might be originated from bacteria via horizontal gene transfer. Transcriptomic dynamics tracked via messenger RNA sequencing over six time points during metabolic switch from starch-rich heterotrophy to lipid-rich photoautotrophy revealed that under heterotrophy, genes most strongly expressed were from the tricarboxylic acid cycle, respiratory chain, oxidative phosphorylation, gluconeogenesis, glyoxylate cycle, and amino acid metabolisms, whereas those most down-regulated were from fatty acid and oxidative pentose phosphate metabolism. The shift from heterotrophy into photoautotrophy highlights up-regulation of genes from carbon fixation, photosynthesis, fatty acid biosynthesis, the oxidative pentose phosphate pathway, and starch catabolism, which resulted in a marked redirection of metabolism, where the primary carbon source of glycine is no longer supplied to cell building blocks by the tricarboxylic acid cycle and gluconeogenesis, whereas carbon skeletons from photosynthesis and starch degradation may be directly channeled into fatty acid and protein biosynthesis. By establishing the first genetic transformation in industrial oleaginous C. pyrenoidosa, we further showed that overexpression of an NAD(H) kinase from Arabidopsis (Arabidopsis thaliana) increased cellular lipid content by 110.4%, yet without reducing growth rate. These findings provide a foundation for exploiting the metabolic switch in microalgae for improved photosynthetic production of food and fuels.

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Section: Dynamics Of the Central Metabolism That Supports The Switchmentioning

confidence: 99%

Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.

et al. 2015

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“…Researchers have been trying to manipulate the algae biomass by various physiochemical parameters, genetic engineering, light sensitive photo-bioreactors etc. Various stress conditions have been studied with different experimental setups to obtain the desired lipid in high amount for biodiesel production (Sharma et al, 2012;Fan et al, 2014). Moreover an alga has the potential to survive and manipulate the lipid metabolic pathways under varying physiochemical conditions (Mohammed et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of Salinity, pH, Light Intensity on Growth and Lipid Production of Microalgae for Bioenergy Application

Prakash¹,

Gautom²,

Sharma³

2015

OnLine Journal of Biological Sciences

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The crisis of energy producing molecules (fuels) is expected to increase in future, which is currently produced from crude mineral oil. Biodiesel is most reliable, non-toxic, biocompatible liquid fuel that can replace the existing unsustainable sources of energy. Among all the known sources, microalgae display high potential for the production of biodiesel owing to their numerous benefits like higher biomass productivities than plants, no agricultural land requirement, cultivation in waste water and accumulation of 20-50% triacylglycerols. Microalgae biomass and lipid content plays an important role in commercial production of biodiesel. The present work was carried out to develop an axenic culture of a potential microalga Chlorella sp. for high biomass and enhanced lipid accumulation. The important growth parameters like pH, light colour, light intensity and photoperiod were studied for better production of Chlorella biomass. The effect of salinity on cell growth was also studied and compared with normal Fogg's medium grown cells. The main biomolecules like carbohydrate, protein, lipid and chlorophyll content were also estimated with the help of standard biochemical methods in salt supplemented and without salt Fogg's medium. The cellular lipid content was increased by growing the cells under different salt concentrations. The micro algal strain showed highest growth of 0.822 g L −1 and 1.021g L −1 in Fogg's medium and under 0.2 M NaCl supplemented medium respectively. However, the maximum lipid production of 0.1842 g L −1 was estimated by growing the cells in Fogg's medium including 0.5 M NaCl with slight compromise in cell growth (0.858 g L −1 ). The lipid content of Chlorella sp. was found to be 26.84% as compared to 14% obtained under normal culture condition. Thus, growing Chlorella sp. under salt supplemented medium and optimizing light requirement will produce high biomass and oil for biodiesel production.

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“…The KH 2 PO 4 in Chlorella nutrient medium and K 2 HPO 4 in Prat nutrient medium is the source of phosphate for algal growth. Fan, J., et al [34] conducted a study upon Chlorella pyrenoidosa and reported that the low phosphorus concentration for seven days decreased biomass.…”

Section: Resultsmentioning

confidence: 99%

Increasing the Protein Amount of Chlorella vulgaris [Chlorphyta] Strains Isolated from Different Fresh Water Ponds

Duygu¹

2017

JESE-B

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Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chosen among 11 C. vulgaris strains isolated from different fresh water ponds and their cell numbers and the amount of chlorophyll a, protein, lipid, cellulose and carbohydrate were examined. The main goal of the study is to investigate increasing the biochemical contents especially the protein content of C. vulgaris strains in different mediums. In the present study, cell densities were determined through cell count for five days. In parallel with cell count, their chlorophyll a content was determined. The highest cell density was observed with C. vulgaris TOH (Tourism and Hotel Management Pond) strain as 5.5 × 10 4 h/mL, and the chlorophyll a content as 4.3 × 10 2 mg/m 3 . The highest intracellular protein amount was determined with C. vulgaris GUH (Gazi University Rectorship Pond) (0.061 g/100 mL) and the highest lipid amount was attained with C. vulgaris UIK (Ulus Construction Well) strain as 0.019 g/100 mL. The process of increasing the intracellular protein amount in C. vulgaris GUH strain was carried out in Prat, Yagojinski and Chlorella medium. The results indicated that Chlorella medium increased the intracellular protein amount.

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Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosaunder three nutrition stressors

Cited by 270 publications

References 54 publications

Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.

Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.

Effect of Salinity, pH, Light Intensity on Growth and Lipid Production of Microalgae for Bioenergy Application

Increasing the Protein Amount of Chlorella vulgaris [Chlorphyta] Strains Isolated from Different Fresh Water Ponds

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