Lipases for biotechnology

Jaeger, Karl‐Erich; Eggert, Thorsten

doi:10.1016/s0958-1669(02)00341-5

Cited by 1,219 publications

(744 citation statements)

References 56 publications

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“…For the present study, the extracellular lipase producing fungus was isolated from spent bleaching earths in sterile bottles and plastic bags by dilution plate method [33].…”

Section: Sample Collectionmentioning

confidence: 99%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

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Abstract. The present investigation was carried out to evaluate the effect of different growth conditions on lipase production by Aspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimizations of physical and chemical parameters were done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 -9), incubation temperature (20 -30 °C), incubation time (8 -80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity by Aspergillus niger was 200 rpm.

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“…For the present study, the extracellular lipase producing fungus was isolated from spent bleaching earths in sterile bottles and plastic bags by dilution plate method [33].…”

Section: Sample Collectionmentioning

confidence: 99%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

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“…They are also involved in acidolysis, alcoholysis and aminolysis [4]. These reactions are of industrial applications in food processing, organic chemical processing, pharmaceuticals, cosmetics [5], paper manufacture, detergent formulations and in environmental management [2,6]. Microbial lipases are of wide interest because they are highly selective, stable and substrate specific [7].…”

Section: Introductionmentioning

confidence: 99%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated from the contaminated soil of a palm oil processing shed. This was with a view to obtaining a strain that can secrete lipase with biochemical properties exploitable for biotechnological applications such as bioremediation of oil contaminated sites. Soil samples were collected from palm oil contaminated sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´ and Longitude E 4°20.7587´). The isolated fungal strains were screened on tributyrin agar for exogenous lipolytic activity. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Rhizopus oryzae ZAC3 (NCBI accession No: KX035094) was identified as the highest lipaseproducing strain. Maximum lipase production was observed on the fourth day, pH 5.0 and a temperature of 45 o C. Olive oil, xylose and yeast extract were the best inducer, carbon and nitrogen sources respectively for lipase production. There was a 2.02 fold increase in lipase production under these optimized conditions. In conclusion, Rhizopus oryzae ZAC3 lipase has properties exploitable for industrial and biotechnological applications.

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“…Novel biotechnological applications have been successfully established using lipase for the synthesis of biopolymers and biodiesel, the production of enantiopure pharmaceuticals, agro-chemicals and fl avor compounds [1][2][3][4][5][6]. The advantages of enzymatic hydrolysis over a chemical process are less energy requirement and higher quality of obtained product.…”

Section: Introductionmentioning

confidence: 99%

Extracellular lipase of Aspergillus niger NRRL3; production, partial purification and properties

Adham

Ahmed

2009

Indian J Microbiol

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Four strains of Aspergillus niger were screened for lipase production. Each was cultivated on four different media differing in their contents of mineral components and sources of carbon and nitrogen. Aspergillus niger NRRL3 produced maximal activity (325U/ml) when grown in 3% peptone , 0.05% MgSO 4 .7H 2 O, 0.05% KCl, 0.2% K 2 HPO 4 and 1% olive oil:glucose (0.5:0.5). A. niger NRRL3 lipase was partially purifi ed by ammonium sulphate precipitation. The majority of lipase activity (48%) was located in fraction IV precipitated at 50-60% of saturation with a 18-fold enzyme purifi cation. The optimal pH of the partial purifi ed lipase preparation for the hydrolysis of emulsifi ed olive oil was 7.2 and the optimum temperature was 60°C. At 70°C, the enzyme retained more than 90% of its activity. Enzyme activity was inhibited by Hg 2+ and K + , whereas Ca 2+ and Mn 2+ greatly stimulated its activity. Additionally, the formed lipase was stored for one month without any loss in the activity.

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Lipases for biotechnology

Cited by 1,219 publications

References 56 publications

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Extracellular lipase of Aspergillus niger NRRL3; production, partial purification and properties

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