Lipase Production from Bacillus subtilis using various Agricultural waste

Abbas, Naaz; Javed, J.; Abbas, Zainab A.; Choudry, Shahnaz; Ali, Sakhawat

doi:10.24001/ijaems.3.5.1

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…The lipase production of isolate I 13 at pH 7 is due to its cold-adapted characteristics. Abbas [44] also reported maximum lipase production at pH 7.0 by Bacillus subtillis. Many cold-adapted lipase-producing bacterial strains like Pseudomonas antartica sp., P. meridiana sp., Acinetobacter sp., Aeromonas sp., etc show lipase activity in neutral to alkaline pH [19].…”

Section: Discussionmentioning

confidence: 91%

Screening, optimization, and molecular characterization of cold-active lipase producing Bacillus cereus I13 from Arctic sediments

Kattatheyil,

Sajeela,

Kabeer

et al. 2024

Biologia

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Cold active enzymes are valuable biocatalysts, due to their capacity to withstand extreme conditions.Microbial lipases are widely used in biotechnological applications as well as in industries due to their broad substrate speci city, and higher stability with less production costs compared to other sources. In the present study, bacterial strains were isolated from the surface and sub-surface sediment samples of the Arctic fjord and were screened for lipase enzyme production. From the 73 isolates that were screened, 8 isolates are considered to be good lipase producers. Among the 8 isolates, Bacillus cereus I13 produced lipase with the activity of 11.42 U/ml on the 4th day of incubation at 20 °C along with the highest zone of clearance of 27 mm in the agar well diffusion plate method. The optimum condition for higher lipase production was at pH 7.0, a temperature of 20 °C, an incubation period of 96 hours with glucose as a carbon source, yeast as the nitrogen source, and olive oil as substrate. Bacillus cereus I13 from Arctic fjord sediments can be used as a potential candidate for the industrial production of cold-active lipase.The optimization studies described in the study proved that lipase production can be effectively increased using these potent isolates.

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Section: Discussionmentioning

confidence: 91%

Screening, optimization, and molecular characterization of cold-active lipase producing Bacillus cereus I13 from Arctic sediments

Kattatheyil,

Sajeela,

Kabeer

et al. 2024

Biologia

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“…The profiles obtained in this study, may vary if the medium and culture conditions are varied. In fact, various factors influence enzyme production and bacterial growth, including the type and concentration of nutrients, the pH of the medium, the incubation temperature, inoculums' size, the presence or absence of metallic ions and inductors (Abbas et al, 2017;Shyamala Devi et al, 2012;Sharma et al, 2014). For this reason, it is necessary to optimize the culture parameters for each strain, in order to increase the cell growth and the level of lipase production.…”

Section: Bacterial Lipase Production In Liquid Mediummentioning

confidence: 99%

Microorganisms Isolated from Moroccan Olive-Mill Wastes: Screening of Their Enzymatic Activities for Biotechnological Use

Misbah

Aouine

Raouan

et al. 2019

ESJ

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In this study, three samples of olive oil wastes: Olive Rinse Water (OR), Olive Mill Wastewater (OM) and Olive Pomace (OP), which were collected from an olive oil mill located at Fez-Morocco, were analyzed for their microbiota. A total of 81 isolates were obtained and constituted a microbial bank formed of 35 bacteria, 41 yeasts and 5 fungi. The microbial communities have been compared in term of metabolic potential by testing hydrolytic enzymes activities of lipase, protease, amylase, cellulase, pectinase and tannase on agar plate media. The results reveal that among the examined microorganisms, 68 isolates were able to produce at least one of the screened enzymes. The pectinase activity was the predominant one (39.51%), followed by cellulase activity (34.57%) and by lipase activity (27.16%). However, the amylase activity was observed only for 11.11% of the studied isolates, followed by protease activity (9.88%) and by tannase activity 6.17%. Thereby, considering the isolates ability to produce enzymes, they can be considered as potential candidates for industrial and biotechnological applications. Lipases

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Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Ikhwani,

Idris,

Elfirta

et al. 2024

Journal of the Turkish Chemical Society Section A: Chemistry

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Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983 ± 0.101 U/mL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes (2.810 U/mL), pH of 8.0 (3.173 U/mL), and a temperature of 35 oC (3.173 U/mL). These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

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Lipase Production from Bacillus subtilis using various Agricultural waste

Abstract: Abstract-Lipases was produced by

Cited by 4 publications

References 6 publications

Screening, optimization, and molecular characterization of cold-active lipase producing Bacillus cereus I13 from Arctic sediments

Screening, optimization, and molecular characterization of cold-active lipase producing Bacillus cereus I13 from Arctic sediments

Microorganisms Isolated from Moroccan Olive-Mill Wastes: Screening of Their Enzymatic Activities for Biotechnological Use

Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

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