Lipase production by <i>Aspergillus niger</i> using sheanut cake: An optimization study

Salihu, Aliyu; Bala, Manju; Alam, Md. Zahangir

doi:10.1016/j.jtusci.2015.02.011

Cited by 37 publications

(26 citation statements)

References 38 publications

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“…Over the recent years, lipases have emerged as one of the prominent biocatalysts and accounts nearly 10% of the enzyme market (Salihu et al 2016). Fossil fuels are running out of reserves due to enormous usage in vehicular applications.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, the selection of an appropriate substrate is an essential step during the production of enzymes in SSF. Various agro-industrial residues such as wheat bran, soybean cake, rice husk, gingelly oil cake, olive oil cake, sugar cane bagasse, babassu oil cake, and sheanut cake were studied (Salihu et al 2013(Salihu et al , 2016Fleuri et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

et al. 2019

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Background: Lipases are serine hydrolases that degrade triglycerides, an attribute that treasures wide applications in biodiesel production, detergent, chemical industries, etc. The most sought after the application is in the high quality and economical production of biodiesel under mild reaction conditions and simplified product separation. For the said application, fungal lipases are ideal catalysts that could effectively catalyze esterification and transesterification reactions with their specific ability to release fatty acids from 1, 3 positions of acylglycerols. Results: In the present work, to facilitate bulk synthesis, lipase production using Aspergillus niger MTCC 872 was studied by solid-state fermentation (SSF). The chosen fungal strain was evaluated for lipase production using a mixture of agroindustrial substrates viz. rice husk, cottonseed cake, and red gram husk in various combinations at flask level. Tri-substrate mixture (rice husk, cottonseed cake, and red gram husk) combined in the ratio of 2:1:1 has shown the maximum lipase activity 28.19 U/gds at optimum cultivation conditions of temperature 40°C, moisture content 75% (v/w), pH 6.0 and initial spore concentration of 5.4 million spores per mL. Further studies were performed for scale-up of lipase from flask level to lab scale using tray fermenter. Lipase activity was found to be 24.38 U/gds and 21.62 U/gds for 100 g and 1000 g substrate respectively. Conclusion: This is the first report on the production of lipase from Aspergillus niger MTCC 872 using tri-substrate mixture of rice husk (RH), cottonseed cake (CSC), and red gram husk (RGH). Moreover, comparison between individual, binary, and tri-substrate mixture was carried out for which the highest lipase activity was observed for tri-substrate mixture. In addition, comparable results were found when scale-up was performed using tray fermenter. Thus, the current work signifies usage of agro-industrial residues as substrates for enzyme production by solid-state fermentation process as an effective alternative to submerged fermentation for industrial applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

et al. 2019

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“…Pabline et al (2014) optimized the lipase production in submerged fermentation under different nutrient substrates, such as corn liquor, yeast extract, peptone and soybean oil, as well as different sources of sodium, magnesium, potassium and nitrate; the authors identified four strains with high lipase activity, including Penicillium lanosum and three bacterial strains. The use of walnut cake for the synthesis of lipases from A. niger AS-02 (Salihu et al, 2016) and soy molasses, from C. rugosa and Geotrichum candidum has also been proposed (Morais et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Avocado oil as an inducer of the extracellular lipase activity of Kluyveromyces marxianus L-2029

et al. 2020

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Avocado represents one of the most nutritious and consumed foods in Mexico, and its export generates significant profits; because of its fatty acids content, the avocado oil was used as an inducer for the synthesis of extracellular lipases from the non-conventional yeast Kluyveromyces marxianus L-2029. Lipases are able to catalyze acyl glycerides, hydrolyzing them into fatty acids and glycerol; they have several applications in various industries. On order to determine the ideal initial avocado oil concentration in the culture medium for the yeast, the induction was carried out at different concentrations in incubation for 24 h. The yeast presented the greater extracellular lipase productivity at 3.5% v/v avocado oil concentration, with a 3.47 μmol PNF/mg prot * min (U/mL) maximum activity. The enzymatic extract obtained at this condition had an optimum lipase activity temperature of 36 °C and pH 6. The pYJR107W lipase was detected in the enzymatic extract from avocado oil induction, and is therefore responsible for the extracellular lipase activity of K. marxianus.

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“…The microorganism lipases are more useful because its can be produced in large amounts in shorter periods of time (Gopinath et al 2013). Aspergillus niger (Salihu et al 2016), A. flavus, Alternaria sp., Penicillium sp. (Wadia and Jain 2017), Bacillus subtilis (Chaturvedi et al 2010), GeoBacillus stearothermophilus (Ekinci et al 2015), and Lactobacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia

Anggriani¹,

Budiarti²,

Mubarik³

2020

Biodiversitas

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Abstract. Anggriani L, Budiarti S, Mubarik NR. 2020. Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia. Biodiversitas 21: 622-628. One of fish preservation techniques through fermentation is Inasua which occurs spontaneously and involves various types of microorganisms. Isolates from Inasua are believed to have the ability to produce lipolytic enzymes. The purpose of this study was to identify potential bacterial isolates isolated from Inasua and to determine their lipase producing ability. The confirmation test of lipase-producing ability was conducted by inoculating the microbes into media containing Nutrient agar (NA), olive oil, and rhodamin-B. Lipolytic bacteria were detected using a screening method with UV light. From the results, there were 5 isolates of bacteria that showed lipolytic activity as IG 3.1, IG 6, IG 10, IG 12, IG 66. Lipase assay was determined using spectrophotometric method with p-nitrophenyl palmitate (pNPP) as a substrate and titration method with olive oil as a substrate. The enzyme showed maximum activity at pNPP as a substrate. IG 6 and IG 12 gave the highest activity from lipase assay. Growth and production curves were created before partial purification was carried out. The results of the curves showed that the optimum activity of IG 6 and IG 12 were in the stationary phase with value 38.16 U.mg-1 protein and 7.01 U.mg-1 protein, respectively. After ammonium sulfate precipitation, the activity of the lipase IG 6 and IG 12 were found to be 39.65 U.mL-1 and 37.05 U.mL-1, respectively. During optimization, the enzyme showed maximum activity at pH 8/70°C temperature. Molecular identification by using the 16S rRNA gene and phylogenetic analysis showed that isolates IG 6 and IG12 are closely related to Bacillus paramycoides and Bacillus kochii, respectively.

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Lipase production by Aspergillus niger using sheanut cake: An optimization study

Cited by 37 publications

References 38 publications

Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation

Avocado oil as an inducer of the extracellular lipase activity of Kluyveromyces marxianus L-2029

Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia

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