Abstract:Abstract. Anggriani L, Budiarti S, Mubarik NR. 2020. Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia. Biodiversitas 21: 622-628. One of fish preservation techniques through fermentation is Inasua which occurs spontaneously and involves various types of microorganisms. Isolates from Inasua are believed to have the ability to produce lipolytic enzymes. The purpose of this study was to identify potential bacterial isolates isolated from Inasua and to dete… Show more
“…Y2 dari black liquor mampu menghasilkan enzim CMCase, xilanase, protease, amilase dan pullulanase (Yang et al 2010). Penelitian sebelumnya telah dilaporkan bahwa isolat bakteri asal fermentasi ikan-inasua berpotensi menghasilkan enzim protease, yaitu IG31 dan IG12 (Amaliah 2017; Yandi 2017) dan enzim lipase yaitu isolat bakteri IG6 dan IG66 (Anggriani et al 2019), namun hingga saat ini belum diketahui potensinya sebagai penghasil enzim amilase.…”
Section: Pendahuluanunclassified
“…Hal ini mengindikasikan bahwa keempat isolat tersebut diduga merupakan galur bakteri yang berbeda. Sesuai dengan hasil penelitian sebelumnya yang dilakukan oleh Anggriani et al (2019), berdasarkan analisis gen 16S rRNA isolat IG6 dan IG12 masing-masing memiliki kesamaan dengan Bacillus paramycoides dan Bacillus kochii. Identifikasi secara molekuler perlu dilakukan pada penelitian selanjutnya untuk mengetahui identitas dari isolat bakteri IG66 dan IG12.…”
Fish-inasua fermented food is a potential source to obtain halotolerant bacteria-producing amylase. Previously, four bacterial isolates such as IG12, IG6, IG66, and IG31, isolated from this food, have been reported to be able to produce protease and lipase enzyme, but their potential as amylase enzyme producers have not been studied. This study aims to characterize and test the potential of halotolerant bacteria isolated from this food in producing amylase enzymes. Based on Gram staining, four bacterial isolates were Gram-positive. The qualitative test of amylase on NA medium with the addition of 1% starch showed four bacteria were able to produce amylase in NaCl (0 to 5%)-containing medium. IG66 isolate was the most potential isolate because it had the highest enzyme activity in a medium with 5% NaCl concentration, which was 0.095 U/ml. The amylase activity was produced maximum at the 8th hour, which is the early stationary phase. Its maximum activity in the 5% NaCl-containing medium was at the 10th hour. In addition, amylase produced by IG66 isolate reached optimum activity at a temperature of 50°C and pH 7. This study concluded that IG66 isolate was the most potential halotolerant isolate that could be developed as an amylase-producing agent.
“…Y2 dari black liquor mampu menghasilkan enzim CMCase, xilanase, protease, amilase dan pullulanase (Yang et al 2010). Penelitian sebelumnya telah dilaporkan bahwa isolat bakteri asal fermentasi ikan-inasua berpotensi menghasilkan enzim protease, yaitu IG31 dan IG12 (Amaliah 2017; Yandi 2017) dan enzim lipase yaitu isolat bakteri IG6 dan IG66 (Anggriani et al 2019), namun hingga saat ini belum diketahui potensinya sebagai penghasil enzim amilase.…”
Section: Pendahuluanunclassified
“…Hal ini mengindikasikan bahwa keempat isolat tersebut diduga merupakan galur bakteri yang berbeda. Sesuai dengan hasil penelitian sebelumnya yang dilakukan oleh Anggriani et al (2019), berdasarkan analisis gen 16S rRNA isolat IG6 dan IG12 masing-masing memiliki kesamaan dengan Bacillus paramycoides dan Bacillus kochii. Identifikasi secara molekuler perlu dilakukan pada penelitian selanjutnya untuk mengetahui identitas dari isolat bakteri IG66 dan IG12.…”
Fish-inasua fermented food is a potential source to obtain halotolerant bacteria-producing amylase. Previously, four bacterial isolates such as IG12, IG6, IG66, and IG31, isolated from this food, have been reported to be able to produce protease and lipase enzyme, but their potential as amylase enzyme producers have not been studied. This study aims to characterize and test the potential of halotolerant bacteria isolated from this food in producing amylase enzymes. Based on Gram staining, four bacterial isolates were Gram-positive. The qualitative test of amylase on NA medium with the addition of 1% starch showed four bacteria were able to produce amylase in NaCl (0 to 5%)-containing medium. IG66 isolate was the most potential isolate because it had the highest enzyme activity in a medium with 5% NaCl concentration, which was 0.095 U/ml. The amylase activity was produced maximum at the 8th hour, which is the early stationary phase. Its maximum activity in the 5% NaCl-containing medium was at the 10th hour. In addition, amylase produced by IG66 isolate reached optimum activity at a temperature of 50°C and pH 7. This study concluded that IG66 isolate was the most potential halotolerant isolate that could be developed as an amylase-producing agent.
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