Lipase-producing fungi for potential wastewater treatment and bioenergy production

rvio, Tuacute lio Alves Cassini Seacute; Paulo, Wagner Pereira Antunes; Laura, Marina; Regina, D.; Ricardo, Franci Gonccedil alves

doi:10.5897/ajb2015.14666

Cited by 5 publications

(1 citation statement)

References 28 publications

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“…Up to date, some of the most recognized fungal genera reported as lipase and esterase producers are Trichoderma [25]; Aspergillus [26]; Penicillium [27]; Alternaria [28], and Fusarium [29]. They were reported in soil samples collected from different regions [25,30,31], plant species of Cerrado and Atlantic forest [32]; pig manure [33]; crude fragment of a chicken slaughterhouse [34], and oil-contaminated soil [35].…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

et al. 2019

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Recalcitrant characteristics and insolubility in water make the disposal of synthetic polymers a great environmental problem to be faced by modern society. Strategies towards the recycling of post-consumer polymers, like poly (ethylene terephthalate, PET) degradation/depolymerization have been studied but still need improvement. To contribute with this purpose, 100 fungal strains from hydrocarbon-associated environments were screened for lipase and esterase activities by plate assays and high-throughput screening (HTS), using short-and long-chain fluorogenic probes. Nine isolates were selected for their outstanding hydrolytic activity, comprising the genera Microsphaeropsis, Mucor, Trichoderma, Westerdykella, and Pycnidiophora. Two strains of Microsphaeropsis arundinis were able to convert 2-3% of PET nanoparticle into terephthalic acid, and when cultured with two kinds of commercial PET bottle fragments, they also promoted weight loss, surface and chemical changes, increased lipase and esterase activities, and led to PET depolymerization with release of terephthalic acid at concentrations above 20.0 ppm and other oligomers over 0.6 ppm. The results corroborate that hydrocarbon-associated areas are important source of microorganisms for application in environmental technologies, and the sources investigated revealed important strains with potential for PET depolymerization.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

et al. 2019

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Screening and production of lipase from fungal organisms

Geoffry

Achur

2018

Biocatalysis and Agricultural Biotechnology

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Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Helal

Abdelhady

Abou-Taleb

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rhizopus species is among the most well-known lipase producers, and its enzyme is suitable for use in many industrial applications. Our research focuses on the production of lipase utilizing waste besides evaluating its applications. Results An extracellular lipase was partially purified from the culture broth of Rhizopus oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis. The partially purified enzyme was non-specific lipase and the utmost activity was recorded at pH 6, 40 °C with high stability for 30 min. The constants Km and Vmax, calculated from the Lineweaver-Burk plot, are 0.3 mg/mL and 208.3 U/mL, respectively. Monovalent metal ions such as Na+ (1 and 5 mM) and K+ (5 mM) were promoters of the lipase to enhance its activity with 110, 105.5, and 106.5%, respectively. Chitosan was used as a perfect support for immobilization via both adsorption and cross-linking in which the latter method attained immobilization efficiency of 99.1% and reusability of 12 cycles. The partially purified enzyme proved its ability in forming methyl oleate (biodiesel) through the esterification of oleic acid and transesterification of olive oil. Conclusion The partially purified and immobilized lipase from Rhizopus oryzae R1 approved excellent efficiency, reusability, and a remarkable role in detergents and biodiesel production.

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Lipase-producing fungi for potential wastewater treatment and bioenergy production

Cited by 5 publications

References 28 publications

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

Screening and production of lipase from fungal organisms

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

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