Lipase Immobilized on Magnetic Nanoparticles: A New Tool for Synthesis of Disulphide Compounds

Abstract

The study describes chemo-enzymatic synthesis of organic disulphide compounds. The reaction was initiated by hydrolysis of thiol acetates using hydrolytic enzyme lipase (PPL) immobilized on to magnetic nanoparticles and subsequent formation of organic disulphide compounds. Lipase was immobilized on to the magnetic nanoparticles by co-precipitation method via epichlorohydrin chitosan cross-linking, under mild and eco-friendly conditions. The immobilized lipase enzyme exhibited broad range of substrate specificity in synthesizing disulphide compounds, which involves both intra and inter-molecular disulphide bond formation under anaerobic conditions. The disulphide compounds synthesized also show a promising antimicrobial activity.

A review on the important aspects of lipase immobilization on nanomaterials

“…The immobilized lipase hydrolyzed the aromatic and aliphatic thiol acetates into the respective disulfide compounds. The enzymatic hydrolysis process did not generate intermediates products, and disulfide compounds were spontaneously formed by thiol radicals under the maintained anaerobic conditions . In another recent study, lipases from Pseudomonas fluorescens were immobilized on MWCNTs that catalyzed O‐acylation reactions .…”