Lipase immobilized in microemulsion based organogels (MBGs) as an efficient catalyst for continuous-flow esterification of protected fructose

Sutili, Felipe K.; Nogueira, Daniel de O.; Leite, Selma Gomes Ferreira; Miranda, Leandro S. M.; Souza, Rodrigo O. M. A. de

doi:10.1039/c5ra04686f

Cited by 14 publications

(5 citation statements)

References 20 publications

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“…The interfacial adsorption of lipases on hydrophobic supports [ 24 ], covalent bonds, and ionic interactions are among the immobilization techniques reported in this period [ 25 ]. These methods included the use of different supports, such as chitosan, celite, silica magnetic microparticles, octyl silica [ 26 , 27 , 28 , 29 ], and microemulsion-based organogels (MBGs) [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Lipozyme 435-Mediated Synthesis of Xylose Oleate in Methyl Ethyl Ketone

et al. 2021

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In this paper, we have performed the Lipozyme 435-catalyzed synthesis of xylose oleate in methyl ethyl ketone (MEK) from xylose and oleic acid. The effects of substrates’ molar ratios, reaction temperature, reaction time on esterification rates, and Lipozyme 435 reuse were studied. Results showed that an excess of oleic acid (xylose: oleic acid molar ratio of 1:5) significantly favored the reaction, yielding 98% of xylose conversion and 31% oleic acid conversion after 24 h-reaction (mainly to xylose mono- and dioleate, as confirmed by mass spectrometry). The highest Lipozyme 435 activities occurred between 55 and 70 °C. The predicted Ping Pong Bi Bi kinetic model fitted very well to the experimental data and there was no evidence of inhibitions in the range assessed. The reaction product was purified and presented an emulsion capacity close to that of a commercial sugar ester detergent. Finally, the repeated use of Lipozyme 435 showed a reduction in the reaction yields (by 48 and 19% in the xylose and oleic acid conversions, respectively), after ten 12 h-cycles.

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Section: Introductionmentioning

confidence: 99%

Lipozyme 435-Mediated Synthesis of Xylose Oleate in Methyl Ethyl Ketone

et al. 2021

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“…Organogel medium has an application for carrying out enzyme-catalyzed transformation. In a previous study, 2,3:4,5- O -diisopropylidene-β- d -fructopyranose has been esterified with palmitic acid in heptanes by passing the mixture continuously through a bed containing CalB lipase enzyme immobilized in microemulsion-based organogels [143], prepared following the reported methods [144,145].…”

Section: Applicationsmentioning

confidence: 99%

Carbohydrate Derived Organogelators and the Corresponding Functional Gels Developed in Recent Time

Basu¹,

Chakraborty

Ghosh

2018

Gels

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Owing to their multifarious applicability, studies of molecular and supramolecular gelators and their corresponding gels have gained momentum, particularly in the last two decades. Hydrophobic-hydrophilic balance, different solvent parameters, gelator-gelator and gelator-solvent interactions, including different noncovalent intermolecular interactive forces like H-bonding, ionic interactions, π-π interactions, van der Waals interactions, etc., cause the supramolecular gel assembly of micro and nano scales with different types of morphologies, depending on the gelator, solvent, and condition of gelation. These gel structures can be utilized for making template inorganic superstructures for potential application in separation, generation of nanocomposite materials, and other applications like self-healing, controlled drug encapsulation, release and delivery, as structuring agents, oil-spill recovery, for preparation of semi-conducting fabrics, and in many other fields. Sugars, being easily available, inexpensive, and nontoxic natural resources with multi functionality and well-defined chirality are attractive starting materials for the preparation of sugar-based gelators. This review will focus on compilation of sugar derived organogelators and the corresponding gels, along with the potential applications that have been

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“…Therefore, it is necessary to provide suitable hydrophilic–hydrophobic interfaces to enhance enzyme activity. Microemulsion (or reverse micelles) is a widely explored system for improving the extent of the hydrophilic–hydrophobic interfaces for reactants transfer and/or enzyme activation . However, the recyclability of enzyme and product separation from the microemulsion systems are difficult and the presence of surfactants frequently causes enzyme deactivation .…”

Section: Introductionmentioning

confidence: 99%

“…Microemulsion (or reverse micelles) is a widely explored system for improving the extent of the hydrophilic-hydrophobic interfaces for reactants transfer and/or enzyme activation. 13 -15 However, the recyclability of enzyme and product separation from the microemulsion systems are difficult and the presence of surfactants frequently causes enzyme deactivation. 16 Solidification with natural polymers into microemulsion-based organogels can improve the recyclability and facilitate product separation to some extent.…”

Section: Introductionmentioning

confidence: 99%

Lipase based static emulsions as efficient biocatalysts for biodiesel production

Zhou

Jiang

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUND Enzymatic production of biodiesel is an alternative solution to circumvent the problems associated with chemical catalysis. However, the drawbacks, including lower stability and reusability of the lipase, often make this route unfeasible for industrial application. RESULTS A stable biocatalyst system was prepared by trapping an aqueous solution of Burkholderiacepacia lipase (BCL) in bead‐shaped silicone microspheres (named lipase‐based static emulsions). Compared with free BCL, lipase‐based static emulsions showed outstanding stability under vigorous shaking conditions and the thermal stability was improved. The lipase‐based static emulsions were employed to catalyze the transesterification of soybean oil with ethanol for biodiesel production. Under the optimum conditions, about 95.42% of FAEE yield was achieved in 30 h. In addition, the lipase‐based static emulsions presented good reusability, and the FAEE yield remained at more than 70.63% after 15 successive reaction cycles. The lipase‐based static emulsions were also used to catalyze the transesterification of jatropha oil and cottonseed oil with ethanol for biodiesel production, and the maximum FAEE yield was 96.10% and 90.39%, respectively. CONCLUSIONS Lipase‐based static emulsions were developed as robust biocatalysts by trapping an aqueous solution of lipase in silicone microspheres. The lipase‐based static emulsions catalyzed process may be a suitable technique for biodiesel production. © 2016 Society of Chemical Industry

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Lipase immobilized in microemulsion based organogels (MBGs) as an efficient catalyst for continuous-flow esterification of protected fructose

Abstract: Sugar-based surfactants are important esters for the cosmetic, food and pharmaceutical industries due to their intrinsic properties.

Cited by 14 publications

References 20 publications

Lipozyme 435-Mediated Synthesis of Xylose Oleate in Methyl Ethyl Ketone

Lipozyme 435-Mediated Synthesis of Xylose Oleate in Methyl Ethyl Ketone

Carbohydrate Derived Organogelators and the Corresponding Functional Gels Developed in Recent Time

Lipase based static emulsions as efficient biocatalysts for biodiesel production

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