Lipase based static emulsions as efficient biocatalysts for biodiesel production

Abstract

BACKGROUND Enzymatic production of biodiesel is an alternative solution to circumvent the problems associated with chemical catalysis. However, the drawbacks, including lower stability and reusability of the lipase, often make this route unfeasible for industrial application. RESULTS A stable biocatalyst system was prepared by trapping an aqueous solution of Burkholderiacepacia lipase (BCL) in bead‐shaped silicone microspheres (named lipase‐based static emulsions). Compared with free BCL, lipase‐based static emulsions showed outstanding stability under vigorous shaking conditions and the thermal stability was improved. The lipase‐based static emulsions were employed to catalyze the transesterification of soybean oil with ethanol for biodiesel production. Under the optimum conditions, about 95.42% of FAEE yield was achieved in 30 h. In addition, the lipase‐based static emulsions presented good reusability, and the FAEE yield remained at more than 70.63% after 15 successive reaction cycles. The lipase‐based static emulsions were also used to catalyze the transesterification of jatropha oil and cottonseed oil with ethanol for biodiesel production, and the maximum FAEE yield was 96.10% and 90.39%, respectively. CONCLUSIONS Lipase‐based static emulsions were developed as robust biocatalysts by trapping an aqueous solution of lipase in silicone microspheres. The lipase‐based static emulsions catalyzed process may be a suitable technique for biodiesel production. © 2016 Society of Chemical Industry

Biocatalytic Synthesis Using Self‐Assembled Polymeric Nano‐ and Microreactors

“…These biocatalysts in static emulsions displayed much higher catalytic activity and stability than those entrapped in a sol–gel system, especially after the preparation protocol was optimized [91] . Such static emulsions have been successfully deployed to entrap different lipases, including Candida Antarctica lipase A (CalA), [90] Pseudomonas stutzeri lipase (lipase TL), [92] lipase of T. lanuginosa , [93] lipase from Burkholderia cepacia , [94] and hydroxynitrile lyase, [95] indicating their versatility for fabricating biocatalytic systems for a broad range of bioconversions.…”

Construction of a Novel Lipase Catalytic System Based on Hybrid Membranes with Interwoven Electrospun Polyacrylic Acid and Polyvinyl Pyrrolidone Gel Fibers

“…Many studies have been performed on using emulsions (microemulsions, nanoemulsions, Pickering emulsions, etc.) to create sufficient oil–water catalytic interfaces for lipase catalysis [ 19 , 20 , 21 , 22 ]. However, there are shortcomings associated with using traditional emulsions for this purpose.…”

“In-situ” lipase-catalyzed cotton coating with polyesters from ethylene glycol and glycerol

“…Based on these specific properties, lipases are widely applied as bio-catalysts in the textile field [8], food manufacture [15], biomedical and pharmaceutical industry [16], and energy (biodiesel) production [17,18]. Lipases can also be described as bio-catalysts for various organic solvents [19] and emulsions [20]. However, a better polymerization process can be achieved by using a solvent-free enzymatic system without further complex purification process [21,22].…”