Lipase from Penicillium camembertii KCCM 11268: Optimization of solid state fermentation and application to biodiesel production

Malilas, Waraporn; Kang, Seong Woo; Kim, Sung Bong; Yoo, Hah Young; Chulalaksananukul, Warawut; Kim, Seung Wook

doi:10.1007/s11814-012-0132-y

Cited by 25 publications

(10 citation statements)

References 6 publications

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“… 34 , although only Amin and Bhatti 35 found that it increased lipase production. In other cases, olive oil 20 , 29 – 31 , 33 , 35 and soluble starch 29 , 31 were found to stimulate lipase production in solid-state cultures. We also noted that lactose increased protease production, although protease biosynthesis increased in Bacillus cereus 36 and Aspergillus versicolor CJS-98 37 after supplementation with maltose.…”

Section: Resultsmentioning

confidence: 96%

“…While solid-state fermentation is widely used to produce enzymes by fungi, we are aware of only one report to date describing lipase production in solid-state (wheat bran) cultures of P . camemberti 20 . In addition, protease production in this species has not been investigated, although lipase and protease production by solid-state fermentation on various substrates has been reported for other Penicillium species.…”

Section: Resultsmentioning

confidence: 99%

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Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Boratyński

Szczepańska

Grudniewska

et al. 2018

Sci Rep

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The study show usefulness of rapeseed cake, rich in fats and proteins byproduct generated after oil production, which may be used as a microbial medium for lipase and protease biosynthesis. Of 26 different filamentous fungi screened by solid-state fermentation, Penicillium camemberti AM83 was found to abundantly produce lipase and protease. Various process parameters were then optimized to maximize lipase and protease secretion, including carbon and nitrogen source, C/N ratio, metal ions, temperature, moisture content, initial pH, and inoculum size. Lipase production increased approximately 11.2-fold in solid-state cultures on rapeseed cake supplemented with lactose and calcium chloride, alkalinized to pH 8, hydrated to 80%, and inoculated with 1.2 × 106 spores/mL. Similarly, protease production increased approximately 8.4-fold in optimized cultures inoculated with 3.2 × 108 spores/mL, and grown on rapeseed cake with lactose and ammonium sulfate at pH 9 and moisture content 60%. The results highlight the potential economic value of solid-state fermentation on rapeseed cake to produce industrial hydrolases.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Boratyński

Szczepańska

Grudniewska

et al. 2018

Sci Rep

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show abstract

“…Many papers on lipase production using solid-state cultivation of fungi on different low-cost media derived from agroindustry residues have been published thus far, although no data of its successful commercialization on a large scale are available. With the purpose of lipase production, Aspergillus niger was cultivated on a variety of agricultural wastes [6][7][8][9], Rhizopus oryzae on sugarcane bagasse [10], Aspergillus species on olive pomace [11,12], Trichoderma strains on oil-palm fruit-bunch medium [13], Penicillium camemberti KCCM 11268 on wheat bran [14], Schizophyllum commune ISTL04 on Leucaena leucocephala seeds [15], etc.…”

Section: Introductionmentioning

confidence: 99%

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

et al. 2019

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This study shows that by-products obtained after cold-pressing oil production (flex oil cake, hemp oil cake, hull-less pumpkin oil cake) could be used as substrates for the sustainable and cost-effective production of lipase when cultivating Thermomyces lanuginosus under solid-state conditions (T = 45 °C, t = 9 days). Lipase showed optimum activity at T = 40 °C. The produced lipase extract was purified 17.03-folds with a recovery of 1% after gel chromatography. Three different batch experiments were performed in order to test the possibility of using the lipase in biodiesel production. Experiments were performed with a commercial, unpurified enzyme, and partially purified lipase with sunflower oil and methanol as substrates in a batch reactor at 40 °C. During the experiments, the operational stability of the enzyme was studied. The obtained results clearly showed that produced crude and purified lipase can be used for biodiesel production, but the process needs some additional optimization. As for operation stability, it was noticed that the commercial enzyme was deactivated after 30 h, while produced crude enzyme remained 8.25% of its activity after 368 h.

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“…Several studies have been performed on the use of agroindustrial residues as substrates in solid-state fermentation systems (SSF) for the production of value-added products such as microbial lipases (Graminha et al, 2008;Malilas et al, 2013; Acta Scientiarum. Technology Maringá, v. 39, n. 1, p. 1-8, Jan.-Mar., 2017 Mussatto, 2009;Pandey, Soccol, & Mitchell, 2000;Salihu et al, 2012;Santos et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

<b>Lipase from <i>Aspergillus niger</i> obtained from mangaba residue fermentation: biochemical characterization of free and immobilized enzymes on a sol-gel matrix

et al. 2017

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ABSTRACT. In this study, mangaba residue (seeds) was used as a substrate for Aspergillus niger lipase production by solid-state fermentation. The partially purified enzyme was efficiently immobilized in a sol-gel matrix by covalent bonding with an immobilization yield of 91.2%. The immobilized biocatalyst and free lipase had an optimum pH of 2.0 and 5.0, respectively. However, greater stability was obtained at pH 4.0 and 7.0, respectively. The biocatalysts showed stability at the optimum temperature of 55°C, where the residual activity was above 87% after 240 min., of incubation. The lower deactivation constant (k d ) and higher half-life of the immobilized biocatalyst indicated greater thermal stability than those obtained with the free enzyme. The Michaelis Constant (K m ) (77 and 115 mM for free and immobilized lipase, respectively) and maximum reaction rate (V max ) (1250 and 714 U mg -1 for free and immobilized lipase, respectively) indicated that the immobilization process reduced enzyme-substrate affinity. Regarding the operational stability, the biocatalyst showed relative activity above 50% until seven cycles of reuse in olive oil hydrolysis. This novel biocatalyst obtained from a tropical fruit residue showed biochemical characteristics that support its application in future biocatalysis studies.Keywords: fruit residue, sol-gel, biotechnology, enzyme.Lipase de Aspergillus niger obtida a partir da fermentação do resíduo de mangaba: caracterização bioquímica das enzimas livre e imobilizada em matriz de sol-gel RESUMO. Neste estudo, o resíduo de mangaba (sementes) foi utilizado como substrato para a produção de lipase de Aspergillus niger por fermentação em estado sólido. A enzima parcialmente purificada foi eficientemente imobilizada em matriz de sol-gel por ligação covalente visto que o rendimento de imobilização foi de 91,2%. O biocatalisador imobilizado e a lipase livre mostraram pH ótimo de 2,0 e 5,0, respectivamente. Entretanto, a maior estabilidade foi obtida nos pHs 4,0 e 7,0, respectivamente. Os biocatalisadores mostraram estabilidade na temperatura ótima de 55°C em que a atividade residual foi superior a 87% após 240 min., de incubação. A menor constante de desativação (k d ) e o maior tempo de meia-vida do biocatalisador imobilizados demonstraram maior estabilidade térmica do que a obtida pela enzima livre. Os parâmetros constantes de Michaelis (K m ) (77 e 115 mm, para as enzimas livre e imobilizada, respectivamente) e velocidade máxima de reação (V máx ) (1250 e 714 U mg -1 , para as enzimas livre e imobilizada, respectivamente) indicaram que o processo de imobilização reduziu a afinidade enzima-substrato. Em relação à estabilidade operacional, o biocatalisador demonstrou atividade relativa acima de 50% até sete ciclos de reutilização na hidrólise do azeite. Este novo biocatalisador, obtido a partir de um resíduo de fruta tropical, apresentou características bioquímicas que justificam sua aplicação em estudos futuros de biocatálise.Palavras-chave: resíduo de fruta, sol-gel, biotecnologia, enz...

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Lipase from Penicillium camembertii KCCM 11268: Optimization of solid state fermentation and application to biodiesel production

Cited by 25 publications

References 6 publications

Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake

Lipase Production by Solid-State Cultivation of Thermomyces Lanuginosus on By-Products from Cold-Pressing Oil Production

<b>Lipase from <i>Aspergillus niger</i> obtained from mangaba residue fermentation: biochemical characterization of free and immobilized enzymes on a sol-gel matrix

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