Lipase-catalyzed degradation of poly(ε-caprolactone)

Pastorino, Laura; Pioli, Franco; Zilli, Mario; Converti, Attílio; Nicolini, Claudio

doi:10.1016/j.enzmictec.2004.05.005

Cited by 61 publications

(43 citation statements)

References 23 publications

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“…This nanofiber skin grafts achieved with a combination of tissue engineering strategy (e.g., making use of scaffolds) and current viable clinical approach (e.g., ‘gold-standard’ autologous skin micrograft) simultaneously presented the following unique features: i) nanotopographic cues (direct and facilitate cell migration which is not available in the current bioengineered skin products); ii) square arrayed microwells (confine skin islands with a uniform distribution, resulting in better cosmetic appearance after wound healing); iii) large expansion ratio (smaller donor sites needed to cover a large wound area); iv) permanent (not a temporary coverage), v) immediate availability and ease of operation (no delay for the treatment and adhere very well to the wound and thus prevent microskin grafts loss during transplantation which usually occurs in traditional skin grafts on extensive burns); and vi) biosafety (biocompatible materials and autologous tissue without immune rejection) [22]. We chose poly(ε-caprolactone) (PCL) for this study because it can provide the desired biomechanical properties and retain a controllable biodegradability in vivo from several weeks to months by incorporating some enzymes [23]. The degradation products of PCL are nontoxic and can be eliminated from the body in the form of carbon dioxide and water [24].…”

Section: Introductionmentioning

confidence: 99%

Sandwich-type fiber scaffolds with square arrayed microwells and nanostructured cues as microskin grafts for skin regeneration

Xie

Jiang

et al. 2014

Biomaterials

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The paper reports the fabrication of sandwich-type scaffolds consisting of radially-aligned nanofibers at the bottom, nanofiber membranes with square arrayed microwells and nanostructured cues at the top, and microskin tissues in between as microskin grafts for use in skin regeneration. This class of nanofiber scaffolds was able to confine the microskin tissues in the square arrayed wells and simultaneously present nanotopographic cues to the cultured NIH 3T3 fibroblasts and primary rat skin cells, guiding and facilitating their migration in vitro. More importantly, we demonstrated that the sandwich-type transplants exhibited an even distribution of microskin grafts, greatly improved the ‘take’ rate of microskin tissues, and promoted re-epithelialization on wound in vivo. In addition, the void area in the scaffolds was well suitable for exudate drainage in wound. The sandwich-type scaffolds show great potential as microskin grafts for repairing extensive burn injuries and may provide a good solution for the treatment of acute skin defects and chronic wounds including diabetic ulcer, pressure ulcer, and venous stasis ulcer.

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Section: Introductionmentioning

confidence: 99%

Sandwich-type fiber scaffolds with square arrayed microwells and nanostructured cues as microskin grafts for skin regeneration

Xie

Jiang

et al. 2014

Biomaterials

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“…Both C 60 -PCL and PCL were characterized by 1 H NMR, 13 C NMR, MALDI-TOF MS, FT-IR, thermogravimetric analysis and differential scanning calorimetry [15]. In particular, in the 13 C NMR spectrum of C 60 -PCL (Figure 1 [18].…”

Section: Resultsmentioning

confidence: 99%

“…Darwis et al evaluated the degrada-*Corresponding author (email: xlyang@bit.edu.cn) tion of chemically crosslinked PCL by lipase AK, revealing its hydrolytic rate lower than non-crosslinked PCL due to formation of a network structure [12]. Pastorino et al investtigated enzymatic degradation of PCL by three different fungal lipases of C. rugosa, M. miehei and R. delemar, and indicated that M. miehei was the most effective [13]. Sivalingam et al examined the enzymatic behavior of lipase against polylactic acid (PLA), PCL and their mixture [14].…”

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confidence: 99%

Degradation of a fullerene end-capped polycaprolactone by lipase AK

Qi-Yun

Kang

et al. 2010

Chin. Sci. Bull.

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Using titrimetric method, enzymatic behavior of lipase AK against fullerene end-capped polycaprolactone (C 60 -PCL) was studied for the first time, compared with polycaprolactone (PCL). The results showed that degradation of both C 60 -PCL and PCL by lipase followed Mechaelis-Menten equation, with the degradation parameters of PCL and C 60 -PCL were 0.75 mg mL -1 and 0.16 mg mL -1 for K m , and 0.90 mL h -1 and 0.43 mL h -1 for V max , respectively. C 60 -PCL had a slower degradation rate than PCL under the same conditions. These data indicated that the introduction of fullerene enabled PCL hard to be degraded by lipase. fullerene end-capped polycaprolactone, polycaprolactone, lipase AK, titrimetry Citation: Peng Q Y, Kang F, Li J, et al. Degradation of a fullerene end-capped polycaprolactone by lipase AK.

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“…The latter substrate was chosen, instead of the usual olive oil (Pastorino et al, 2004), since hydrolysis of tripropionin yields propionic acid, which diffuses into the aqueous phase surrounding the capsules and may be simply measured by titration, whereas intermediates, such as di-or mono-propionin, are extracted by the organic solvent. The activity of immobilized lipase was measured for different initial substrate concentrations and for different quantities of capsules.…”

Section: Immobilization By Adsorptionmentioning

confidence: 99%

A novel reactive perstraction system based on liquid‐core microcapsules applied to lipase‐catalyzed biotransformations

Wyss¹,

Stockar²,

Marison³

2005

Biotech & Bioengineering

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A novel reactive perstraction system has been developed based on liquid-core capsules, involving an enzyme-catalyzed reaction coupled with simultaneous in situ product recovery. Lipase-catalyzed reactions, hydrolysis of triprionin and nitrophenyl laurate, were selected to test the system and demonstrate the feasibility of immobilization of enzymes to the membranes of liquid-core capsules and the ability to extract hydrophobic products of the reaction within the capsule core. The lipase from Candida rugosa was immobilized to the microcapsules by adsorption and by covalent binding through activation with glutaraldehyde. In both cases improved temperature and operational stability were achieved. Both types of immobilization resulted in a basic shift of the pH optimum for activity, from 7.5 to 9.0. The presence of an organic phase within the capsule core allowed direct product separation and lead to a decrease in product inhibition of the lipase-catalyzed reaction.

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Lipase-catalyzed degradation of poly(ε-caprolactone)

Cited by 61 publications

References 23 publications

Sandwich-type fiber scaffolds with square arrayed microwells and nanostructured cues as microskin grafts for skin regeneration

Sandwich-type fiber scaffolds with square arrayed microwells and nanostructured cues as microskin grafts for skin regeneration

Degradation of a fullerene end-capped polycaprolactone by lipase AK

A novel reactive perstraction system based on liquid‐core microcapsules applied to lipase‐catalyzed biotransformations

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