Degradation of a fullerene end-capped polycaprolactone by lipase AK

Abstract

Using titrimetric method, enzymatic behavior of lipase AK against fullerene end-capped polycaprolactone (C 60 -PCL) was studied for the first time, compared with polycaprolactone (PCL). The results showed that degradation of both C 60 -PCL and PCL by lipase followed Mechaelis-Menten equation, with the degradation parameters of PCL and C 60 -PCL were 0.75 mg mL -1 and 0.16 mg mL -1 for K m , and 0.90 mL h -1 and 0.43 mL h -1 for V max , respectively. C 60 -PCL had a slower degradation rate than PCL under the same conditions. These data indicated that the introduction of fullerene enabled PCL hard to be degraded by lipase. fullerene end-capped polycaprolactone, polycaprolactone, lipase AK, titrimetry Citation: Peng Q Y, Kang F, Li J, et al. Degradation of a fullerene end-capped polycaprolactone by lipase AK.

Fullerene–biomolecule conjugates and their biomedicinal applications

“…The data indicated that the introduction of fullerenes decreased the enzymatic reaction of lipase toward poly(caprolactone). 81 It is worth noting that the synthesis of fullerene-flavonoid conjugates, including C 60 -chalcone 48 ( Figure 10B), C 60 -flavone 49 ( Figure 10C), and C 60 -flavanone 50 ( Figure 10D) and their antioxidant abilities have also been reported by Enes et al, who concluded that these conjugates might be promising leads for future broad-spectrum radical scavengers. 82 Furthermore, these conjugates are advantageous over flavonoids alone due to the traits of fullerenes described above, such as the adaption to active sites of enzymes and transportation across cell membranes.…”

“…81 Using N-substituted 3,4-fulleropyrrolidine with an active hydroxyl group as an initiator, polymerization of epsilon-caprolactone was performed via ring-opening polymerization. The data indicated that the introduction of fullerenes decreased the enzymatic reaction of lipase toward poly(caprolactone).…”