Lipase: A potential biocatalyst for the synthesis of valuable flavour and fragrance ester compounds

Dhake, Kishor P.; Thakare, Dinesh D.; Bhanage, Bhalchandra M.

doi:10.1002/ffj.3140

Cited by 142 publications

(90 citation statements)

References 132 publications

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“…It has been shown that lipase-mediated esterification reaction is influenced by several parameters such as molar ratio of acid to alcohol, type of applied solvent and temperature of reaction mixture (Dhake et al, 2013). In order to evaluate the effect of molar ratio of acid to alcohol on the esterification assisted by the partially purified lipase of B. atrophaeus FSHM2, various molar ratios were applied in the reaction mixtures.…”

Section: Partial Purification Of Lipasementioning

confidence: 99%

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Ameri¹,

Shakibaie²,

Amirpour-R³

et al. 2015

Biotechnology

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A B S T R A C T A thermoalkalophilic lipase producing bacterial strain, identified asBacillus atrophaeus FSHM2 using 16S rDNA sequencing analysis was isolated from salty soil and its lipase was partially purified and characterized. The obtained results revealed that glucose, hazelnut oil, urea and calcium ion positively affected the lipase production by increasing the lipolytic activity to 13582.5, 6270, 4442 and 5505 U LG 1 , respectively compared to that of basal medium (4150 U LG 1 ). The partially purified lipase acted optimally at pH 9 and retained 88.2% of its initial activity after 1 h of incubation at 100°C. A two fold increase in the relative activity of the partially purified lipase was obtained in the presence of 4 M of NaCl. Application of the partially purified lipase for the synthesis of ethyl and methyl valerate in the organic solvent medium (xylene) resulted in 81.6 and 62.4% esterification, respectively, after 24 h of incubation.

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Section: Partial Purification Of Lipasementioning

confidence: 99%

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Ameri¹,

Shakibaie²,

Amirpour-R³

et al. 2015

Biotechnology

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“…In this sense, it should be noted that nonpolar solvents have been reported as less harmful to the enzyme than highly polar ones (Rodrigues and Fernandez-Lafuente, 2010a;Dhake et al, 2013).…”

Section: Introductionmentioning

confidence: 94%

“…In particular, several flavors (short and long chain aliphatic esters) of industrial interest have been obtained by esterification, transesterification or interesterification reactions using lipases (Gandhi, 1997;Alcantara et al, 1998;Abbas and Comeau, 2003;Silva and Jesus, 2003;Hasan et al, 2006), with applications in juices, cheeses, baked goods, candies and beverages, for instance (Mahapatra et al, 2009). The demand for these chemicals has continuously increased at the rate of 4.3% per year (Dhake et al, 2013). Rodrigues and Fernandez-Lafuente (2010b) and Dhake et al (2013) presented relevant reviews concerning the wide application of lipases for the synthesis of flavors.…”

Section: Introductionmentioning

confidence: 99%

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Stability of immobilized Rhizomucor miehei lipase for the synthesis of pentyl octanoate in a continuous packed bed bioreactor

Skoronski

Padoin

Soares

et al. 2014

Braz. J. Chem. Eng.

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-The enzymatic synthesis of organic compounds in continuous bioreactors is an efficient way to obtain industrially important chemicals. However, few works have focused on the study of the operational conditions and the bioprocess performance. In this work, the aliphatic ester pentyl octanoate was obtained by direct esterification using a continuous packed bed bioreactor containing the immobilized enzyme Lipozyme ® RM IM as catalyst. Enzymatic deactivation was evaluated under different conditions for the operational parameters substrate/enzyme ratio (5.00, 1.67, 0.83 and 0.55 mmol substrate ·min -1 ·g -1 enzyme ) and temperature (30, 40, 50 and 60 °C). The optimal condition was observed at 30 ºC, which gave the minimum enzymatic deactivation rate and the maximum conversion to the desired product, yielding approximately 60 mmols of ester for an enzyme loading of 0.5 g into the bioreactor. A first-order deactivation model showed good agreement with the experimental data.

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“…Lipases are widely used because of their ready availability, low cost of production, utility in food, biotechnology, and pharmacology [61]. Novel biotechnological applications have been successfully established using lipases for the synthesis phenolic lipids, the production of pharmaceuticals, agrochemicals, and lavor compounds [52,62,63]. Moreover, the use of lipases in the food industry is increasing due to the need for the production of esters, biodegradable polyesters, and speciic FAs [64].…”

Section: Deinition Sources and Applicationsmentioning

confidence: 99%

Synthesis and Characterization of Phenolic Lipids

Roby¹

2017

Phenolic Compounds - Natural Sources, Importance and Applications

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Omega-3 polyunsaturated faty acids (ω3 PUFAs) from ish oils promote well-established health and antiaging beneits that justify their use as functional ingredients in dietary supplements, healthy foods, and nutraceutical products. Dietary supply is needed because human metabolism exhibits limited to synthesize ω3 PUFAs. However, the practical use of such lipids as food ingredients is often limited due to their high susceptibility to oxidation, which is responsible for the undesirable of-lavor and odor of rancid oils, associated with the loss of nutritional value. Produced phenolic lipids were a good solution for all these problems. These phenolic lipids are preferentially produced by enzymatic bioprocesses that exhibit high selectivity toward polyfunctional substrates and mild reaction conditions compared with chemical synthesis pathways. This chapter presents the acylation of phenolic compounds and lipids using enzyme under various operating conditions. In conclusion, the acylation of lipids with natural phenolic compounds resulted in the formation of a lipophilic ester that should be able to stabilize the oil, fats, and emulsions against oxidation. Acylation of lipids to phenolic compounds that have antioxidant properties thus protects the lipids from oxidation, and the phenolic lipid derivatives carry the combined health beneicial properties of lipids and the phenolic molecules.

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Lipase: A potential biocatalyst for the synthesis of valuable flavour and fragrance ester compounds

Cited by 142 publications

References 132 publications

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Stability of immobilized Rhizomucor miehei lipase for the synthesis of pentyl octanoate in a continuous packed bed bioreactor

Synthesis and Characterization of Phenolic Lipids

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