Linoleic acid increases adhesion, chemotaxis, granule release, intracellular calcium mobilisation, MAPK phosphorylation and gene expression in bovine neutrophils

Huertas, Jaqueline Mena; Manosalva, Carolina; Ramirez, Ruben D.; Chandia, Lhia; Carroza, Daniel; Loaiza, Anitsi; Burgos, Rafael A.; Hidalgo, María A.

doi:10.1016/j.vetimm.2012.11.017

Cited by 21 publications

(32 citation statements)

References 39 publications

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“…This effect was acompained by an elevation in the expression of neutrophil adhesion molecules (L-selectin) and increase in endothelium-neutrophil interactions (Mena et al, 2013;Rodrigues et al, 2010).…”

Section: Omega 6 (N-6) Fatty Acidsmentioning

confidence: 98%

Fatty acids as modulators of neutrophil recruitment, function and survival

Rodrigues

Sato

Curi

et al. 2016

European Journal of Pharmacology

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Section: Omega 6 (N-6) Fatty Acidsmentioning

confidence: 98%

Fatty acids as modulators of neutrophil recruitment, function and survival

Rodrigues

Sato

Curi

et al. 2016

European Journal of Pharmacology

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“…To analyse the COX-2 levels, neutrophils were incubated with vehicle (0.1% DMSO), 10 mM GW1100 or 50 mM andrographolide (NF-kB inhibitor) for 15 min at 37 C, and then FFAR1/GPR40 agonists (10 mM GW9508, 100 mM LA or 300 mM OLA) were added and incubated for 3 h at 37 C. Total proteins were obtained, as previously described. 9 Total proteins (80 mg) were analysed by 12% SDS-PAGE and transferred onto a nitrocellulose membrane. Immunoblotting was performed according to a protocol as previously described.…”

Section: Immunoblotmentioning

confidence: 99%

“…6,7 In bovine neutrophils, the unsaturated fatty acids oleic (OLA) and linoleic (LA) acid increase ROS production, matrix metalloproteinase (MMP)-9 release, and cyclooxygenase (COX)-2 and IL-8 mRNA levels. [8][9][10] OLA and LA-induced ROS and MMP-9 release are mediated through free fatty acid receptor-1 (FFAR1/ GPR40). FFAR1/GPR40 is a G protein-coupled receptor that has been identified in pancreatic cells, [11][12][13] monkey neurons, 14 breast cancer cells, 15,16 and bovine mammary epithelial cells and neutrophils.…”

Section: Introductionmentioning

confidence: 99%

Differential free fatty acid receptor-1 (FFAR1/GPR40) signalling is associated with gene expression or gelatinase granule release in bovine neutrophils

et al. 2016

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Fatty acids have been recognized as regulators of immune function in addition to their known metabolic role. Long-chain fatty acids bind free fatty acid receptor (FFAR)-1/GPR40, which is expressed on bovine neutrophils, and increase responses such as granule release and gene expression. In this study, we investigated the molecular mechanisms governing the up-regulation of cyclooxygenase-2 (COX-2) and IL-8, as well as matrix metalloproteinase (MMP)-9 granule release in FFAR1/GPR40 agonist-stimulated neutrophils. Our results showed that natural (oleic and linoleic acid) and synthetic (GW9508) FFAR1/GPR40 agonists increased ERK1/2, p38 MAPK and Akt phosphorylation, and that the FFAR1/GPR40 antagonist GW1100 reduced these responses. We evaluated the levels of IκBα, a component of the classical activation pathway of the transcription factor NF-κB, and we observed IκBα reduction after stimulation with FFAR1/GPR40 agonists, an effect that was inhibited by GW1100 or the inhibitors UO126, SB203580 or LY294002. FFAR1/GPR40 agonists increased COX-2 and IL-8 expression, which was inhibited by GW1100 and an NF-κB inhibitor. Finally, the FFAR1/GPR40 agonist-induced MMP-9 granule release was reduced by GW1100 and UO126. In conclusion, FFAR1/GPR40 agonists differentially stimulate neutrophil functions; COX-2 and IL-8 are expressed after FFAR1/GPR40 activation via NF-κB, IκBα reduction is FFAR1/GPR40- and PI3K/MAPK-dependent, and MMP-9 granule release is FFAR1/GPR40- and ERK1/2-dependent.

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“…Estas espécies reativas por sua vez causam a liberação do citocromo c (Cyt c) que desencadeia a apoptose (REPALLI, 2014), um mecanismo celular muito relacionado na literatura com a modulação do 57 TSPO (MAASER et al, 2001;EVERETT et al, 2002 CANTOR et al, 1984;PHYTON et al, 1993). O cálcio representa um segundo mensageiro chave para várias respostas neutrofílicas (BARRITT, 1999;MENA et al, 2013), entre elas a quimiotaxia, um passo fundamental no processo inflamatório (WONG, 2010). Quanto a esta função, os resultados apresentados mostram que o LTB4 induz a quimiotaxia de neutrófilos, como já descrito na literatura (HENDERSON, 1994;HEBERT, 1996), e que tratamento com Diazepam e PK-11195 reduzem a quimiotaxia de neutrófilos estimulados pelo LTB4.…”

Section: Discussionunclassified

Avaliação dos efeitos dos ligantes de TSPO (translocator protein 18 KDa) na ativação dos neutrófilos

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Linoleic acid increases adhesion, chemotaxis, granule release, intracellular calcium mobilisation, MAPK phosphorylation and gene expression in bovine neutrophils

Cited by 21 publications

References 39 publications

Fatty acids as modulators of neutrophil recruitment, function and survival

Fatty acids as modulators of neutrophil recruitment, function and survival

Differential free fatty acid receptor-1 (FFAR1/GPR40) signalling is associated with gene expression or gelatinase granule release in bovine neutrophils

Avaliação dos efeitos dos ligantes de TSPO (translocator protein 18 KDa) na ativação dos neutrófilos

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