Linker-free covalent immobilization of nisin using atmospheric pressure plasma induced grafting

Aveyard, Jenny; Bradley, James W.; McKay, Kirsty; McBride, Fiona; Donaghy, D.; Raval, Rasmita; D’Sa, Raechelle A.

doi:10.1039/c7tb00113d

Cited by 33 publications

(35 citation statements)

References 55 publications

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“…Nisin is an antimicrobial peptide generally recognized as safe (GRAS) by the US Food and Drug Administration and commercially used as natural preservative [24]. Nisin has been used for the control of gram-positive pathogens, especially L. monocytogenes, in variety of products such as cheese, meat, and canned foods, either as additives in food directly [25] or incorporation with packaging materials [26,27] by means of extrusion [28], layer-by-layer adsorption [29,30], covalently immobilization [31,32], and surface coating [33]. As micro-organisms grow primarily at the surfaces of the foods in most packaged solid or semi-solid foods, antimicrobial activity should be preferably exerted on the surfaces.…”

Section: Introductionmentioning

confidence: 99%

Application of Nanofibrillated Cellulose on BOPP/LDPE Film as Oxygen Barrier and Antimicrobial Coating Based on Cold Plasma Treatment

Lü

Guo

et al. 2018

Coatings

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The application of nanofibrillated cellulose (NC) films in packaging industry has been hindered by its lack of heat-sealing ability. Incorporation of NC films with the biaxially oriented polypropylene/low density polyethylene (BOPP/LDPE) laminates can take advantage of each material and endow the films with novel functions for food packaging applications. In this study, a coating that consists of NC and nisin was applied onto a cold plasma treated BOPP/LDPE film to fabricate a novel active packaging with an improved oxygen barrier performance and an added antimicrobial effect. The results showed that cold plasma treatment improved the surface hydrophilicity of BOPP/LDPE films for better attachment of the coatings. NC coatings significantly enhanced oxygen barrier property of the BOPP/LDPE film, with an oxygen transmission rate as low as 24.02 cc/m 2 •day as compared to that of the non-coated one (67.03 cc/m 2 •day). The addition of nisin in the coating at a concentration of 5 mg/g caused no significant change in barrier properties but imparted the film excellent antimicrobial properties, with a growth inhibition of L. monocytogenes by 94%. All films exhibit satisfying mechanical properties and transparency, and this new film has the potential to be used as antimicrobial and oxygen barrier packaging.

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Section: Introductionmentioning

confidence: 99%

Application of Nanofibrillated Cellulose on BOPP/LDPE Film as Oxygen Barrier and Antimicrobial Coating Based on Cold Plasma Treatment

Lü

Guo

et al. 2018

Coatings

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“…However, most 2 International Journal of Polymer Science of the works describe nisin application on plasma activated polymer surface modifier [13] or in matrix of water-soluble polymers [14,15]. The thermoplastic incorporation into thermoplastic starch matrix has been also reported [16].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers

Holčapková

Rašková

Hrabalíková

et al. 2017

International Journal of Polymer Science

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This work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride and nisin extracted and stabilized by using ammonium sulfate and polysorbate 80.The stability of samples was tested by means of long-term storage at −18, 4, 25, and 55 ∘ C up to 165 days. The nisin content in the samples was determined by high-performance liquid chromatography and electrophoresis. In addition, effect of whey fortification with lactose on nisin production and antibacterial activity studied against Staphylococcus aureus was tested. Results show that stabilization by polyethylene glycol provides enhanced nisin activity at 55 ∘ C after 14 days and long-term stability at 25 ∘ C with keeping antibacterial activity.

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“…These results indicated that nisin was coated on the surface of PN45 and PN60 films. In the study by Aveyard, nisin was immobilized on the polystyrene film by induced grafting after atmospheric pressure plasma treatment, which could not be observed from the FTIR spectra. This shows that the amount of nisin immobilized on PLA with CPT was insignificant and a great mass of nisin was deposited on the film surface.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial activity of nisin‐coated polylactic acid film facilitated by cold plasma treatment

Wei

et al. 2018

J of Applied Polymer Sci

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In this investigation, the surface functionalization of polylactic acid (PLA) film has been carried out by cold plasma treatment (CPT) to facilitate the coating of nisin on its surface. The physical and chemical properties of PLA films, such as surface morphology, water contact angle (WCA), chemical structure, and dynamic mechanical thermal properties, after CPT were systematically investigated. The obtained results demonstrated that the oxygen/carbon (O/C) ratio of PLA films decreased from 0.46 to 0.40 with an increase in the time of CPT. As the time of CPT increased, the roughness at first increased to 6.24 nm for PLA30, but then decreased to 2.26 nm for PLA60, whereas WCA at first decreased to 51.06 but then increased to 75 . Attenuated total reflectance Fourier transform infrared spectroscopy confirmed the successful coating of nisin onto PLA films with CPT. As compared with the PLA film, PN60 decreased the total viable count of Listeria monocytogenes to 2.25 log 10 [CFU/mL]. The different antimicrobial abilities against L. monocytogenes indicated that CPT could affect the adsorption capacity of PLA surface.

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Linker-free covalent immobilization of nisin using atmospheric pressure plasma induced grafting

Abstract: The linker-free covalent immobilization of polymers on surfaces has the potential to impart new properties and functions to surfaces for a wide range of applications.

Cited by 33 publications

References 55 publications

Application of Nanofibrillated Cellulose on BOPP/LDPE Film as Oxygen Barrier and Antimicrobial Coating Based on Cold Plasma Treatment

Application of Nanofibrillated Cellulose on BOPP/LDPE Film as Oxygen Barrier and Antimicrobial Coating Based on Cold Plasma Treatment

Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers

Antimicrobial activity of nisin‐coated polylactic acid film facilitated by cold plasma treatment

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