Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers

Holčapková, Pavlína; Rašková, Zuzana Kolářová; Hrabalíková, Martina; Šalaková, Alexandra; Drbohlav, Jan; Sedlařík, Vladimír

doi:10.1155/2017/3072582

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…Conversely, the peptide antibiotic nisin was more sensitive to temperature, proteolytic enzymes, and a basic pH. This result is consistent with previous stability studies, demonstrating that nisin was stable at −18 • C but substantially degraded at temperatures above 25 • C [33]. The antibacterial activity of nisin was also shown to be diminished when treated with proteases or a pH above 10 because of the presence of enzymatic and chemical cleavage sites in the peptide antibiotic [34,35].…”

Section: Discussionsupporting

confidence: 90%

Promising Anti-MRSA Activity of Brevibacillus sp. Isolated from Soil and Strain Improvement by UV Mutagenesis

2020

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Antibiotic-resistant infection is a major health problem, and a limited number of drugs are currently approved as antibiotics. Soil bacteria are promising sources in the search for novel antibiotics. The aim of the present study is to isolate and assess soil bacteria with anti-MRSA activity and improve their capabilities by UV mutagenesis. Soil samples from the upper south of Thailand were screened for antibacterial activity using the cross-streak method. Agar well diffusion was used to examine the activity of isolates against a spectrum of human pathogens. The most active isolate was identified by 16S rRNA sequencing, and the production kinetics and stability were investigated. The most promising isolate was mutated by UV radiation, and the resulting activity and strain stability were studied. The results show that isolates from the cross-streak method could inhibit Staphylococcus aureus TISTR 517 (94 isolates) and Escherichia coli TISTR 887 (67 isolates). Nine isolates remained active against S. aureus TISTR 517 and MRSA, and eight isolates inhibited the growth of E. coli TISTR 887 as assessed using agar well diffusion. The most active strain was Brevibacillus sp. SPR-20, which had the highest activity at 24 h of incubation. The active substances in culture supernatants exhibited more than 90% activity when subjected to treatments involving various heat, enzymes, surfactants, and pH conditions. The mutant M201 showed significantly higher activity (109.88–120.22%) and strain stability compared to the wild-type strain. In conclusion, we demonstrate that soil Brevibacillus sp. is a potential resource that can be subjected to UV mutagenesis as a useful approach for improving the production of anti-MRSA in the era of antibiotic resistance.

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Section: Discussionsupporting

confidence: 90%

Promising Anti-MRSA Activity of Brevibacillus sp. Isolated from Soil and Strain Improvement by UV Mutagenesis

2020

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“…Nisin has been reported as an effective antimicrobial additive to polyolefins, polyesters, and vinyl‐based polymers via surface and bulk modifications . However, the biological activity of nisin is often constrained by time limitations; when it decomposes, the antibacterial properties of the polymer system are diminished . Several works have described a possible specific interaction of proteins, including nisin, with polyethylene glycol (PEG), as a means of protecting the biofunctionality of the same against external factors such as fluctuations in pH, increased temperature, and ionic strength .…”

Section: Introductionmentioning

confidence: 99%

Effect of polyethylene glycol plasticizer on long‐term antibacterial activity and the release profile of bacteriocin nisin from polylactide blends

Holčapková

Hurajová

Kucharczyk

et al. 2018

Polymers for Advanced Techs

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This work describes the synergetic effect of polyethylene glycol (PEG) in polylactide (PLA) blends, wherein the polyether acts as both the plasticizer and functional additive, ensuring the long-term antimicrobial activity of bacteriocin nisin. Two types of PEG with the molecular weights of 1000 and 6000 g.mol −1 (20 wt.%) were used to plasticize the PLA blends. The aforementioned bacteriocin nisin, at concentrations ranging between 0.02 and 0.15 wt.% (8000-60 000 IU.g −1 ), was incorporated into the samples by the solvent cast technique. The effect of various PEG on the structural, mechanical, and thermal properties of the PLA-based blends were investigated by scanning electron microscopy, Fourier transform infrared spectroscopy, stressstrain analysis, differential scanning calorimetry, and dynamic mechanical analysis, respectively. The antibacterial activity of the samples was detected by the agar diffusion technique against Micrococcus luteus. Furthermore, the antibacterial properties of the samples were tested according the ISO 22196 standard against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus, Listeria monocytogenes) bacterial strains. The nisin was detected by high performance liquid chromatography, the device having been equipped with a UV/vis detector. The results show that the PEG, besides its plasticizing effect, significantly enhances the release profile and sustains long-term antibacterial activity of nisin in a PLA matrix.

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“…They thus need to be modified or functionalized to ensure stability in physiological solutions. The stability of nisin, a frequently used variant for biomedical applications as an antibacterial agent or food preservative, is dependent on the environmental pH [ 20 ]. For instance, solubilized nisin is active and stable in acidic pH, whereas its solubility decreases in alkaline pH, which hinders its biological activity.…”

Section: Stability Of Bacteriocinsmentioning

confidence: 99%

Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins

Naskar

Kim

2021

Pharmaceutics

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Bacteriocins are antimicrobial peptides or proteinaceous materials produced by bacteria against pathogens. These molecules have high efficiency and specificity and are equipped with many properties useful in food-related applications, such as food preservatives and additives, as well as biomedical applications, such as serving as alternatives to current antibacterial, antiviral, anticancer, and antibiofilm agents. Despite their advantages as alternative therapeutics over existing strategies, several limitations of bacteriocins, such as the high cost of isolation and purification, narrow spectrum of activity, low stability and solubility, and easy enzymatic degradation, need to be improved. Nanomaterials are promising agents in many biological applications. They are widely used in the conjugation or decoration of bacteriocins to augment the activity of bacteriocins or reduce problems related to their use in biomedical applications. Therefore, bacteriocins combined with nanomaterials have emerged as promising molecules that can be used in various biomedical applications. This review highlights the features of bacteriocins and their limitations in biomedical applications and provides a detailed overview of the uses of different nanomaterials in improving the limitations. Our review focuses on the potential applications of nanomaterials combined with bacteriocins as new designer molecules for use in future therapeutic strategies.

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Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers

Cited by 11 publications

References 31 publications

Promising Anti-MRSA Activity of Brevibacillus sp. Isolated from Soil and Strain Improvement by UV Mutagenesis

Promising Anti-MRSA Activity of Brevibacillus sp. Isolated from Soil and Strain Improvement by UV Mutagenesis

Effect of polyethylene glycol plasticizer on long‐term antibacterial activity and the release profile of bacteriocin nisin from polylactide blends

Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins

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