Linkage mapping of wool keratin and keratin-associated protein genes in sheep

McLaren, R.J.; Rogers, Geraldine R.; Davies, Katherine; Maddox, Jill F.; Montgomery, Grant W.

doi:10.1007/s003359900616

Cited by 76 publications

(75 citation statements)

References 12 publications

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“…Sheep KAP7 is encoded by a single gene that does not have introns (McLaren et al, 1997). To date, only one KAP7 gene member has been identified in all samples investigated.…”

Section: Kap7 Gene Polymorphismsmentioning

confidence: 99%

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Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

et al. 2014

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ABSTRACT. High glycine-tyrosine proteins (HGTPs), also known as keratin-associated proteins (KAPs), play a key role in the major structures and mechanical properties of wool fiber. Sheep HGTPs consist of three multigene families: KAP6, KAP7, and KAP8 genes. Polymorphisms of these three genes have been proposed to have important effects on wool fiber traits. The aim of the present study was to identify polymorphisms of the KAP6, KAP7, and KAP8 genes in four sheep breeds, including Chinese Merino superfine wool sheep, Hu sheep, a Merino x Hu crossed breed, and Romney sheep. Polymerase chain reaction (PCR) product direct sequencing, PCR-single-strand conformation polymorphism, and cloned sequencing methods were used to find genetic variation and identify polymorphisms in these genes. The Mutation Surveyor v3.97 software was used to analyze the sequences. These methods revealed six different sequences of the KAP6 gene, two different sequences of the KAP7 gene, and five different sequences of the KAP8 gene. Accordingly, 3439©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 3438-3445 (2014) Polymorphisms of sheep wool keratin-associated protein genes three (with frequencies >1%) single nucleotide polymorphisms (SNPs) of the KAP6 gene, one SNP of the KAP7 gene, and five SNPs of the KAP8 gene were detected. Interestingly, some of these sequences were present in only certain sheep breeds, thereby suggesting that these special allele sequences could be used as candidate genes of wool characteristics in further studies.

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“…Sheep KAP7 is encoded by a single gene that does not have introns (McLaren et al, 1997). To date, only one KAP7 gene member has been identified in all samples investigated.…”

Section: Kap7 Gene Polymorphismsmentioning

confidence: 99%

“…Only one member of the KAP8 gene family has been identified to date (McLaren et al, 1997;Gong et al, 2012). Previous studies have demonstrated abundant variations in the KAP8 gene.…”

Section: Kap8 Gene Polymorphismsmentioning

confidence: 99%

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

et al. 2014

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“…construction of low-density autosomal and X chromosome linkage maps (Crawford et al 1995;Broom et al 1996;Galloway et al 1996;Lord et al 1996;McLaren et al 1997;de Gortari et al 1998). For livestock species, such as sheep and cattle, where the costs of rearing and trait evaluation are high relative to the costs of typing a marker (100-1000 : 1), it has been estimated for quantitative trait loci (QTL) studies and genome scans that the optimum spacing between markers lies between 10 and 30 cM (Darvasi and Soller 1994).…”

mentioning

confidence: 99%

An Enhanced Linkage Map of the Sheep Genome Comprising More Than 1000 Loci

Maddox¹

2001

Genome Research

210

136

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A medium-density linkage map of the ovine genome has been developed. Marker data for 550 new loci were generated and merged with the previous sheep linkage map. The new map comprises 1093 markers representing 1062 unique loci (941 anonymous loci, 121 genes) and spans 3500 cM (sex-averaged) for the autosomes and 132 cM (female) on the X chromosome. There is an average spacing of 3.4 cM between autosomal loci and 8.3 cM between highly polymorphic [polymorphic information content (PIC) Ն 0.7] autosomal loci. The largest gap between markers is 32.5 cM, and the number of gaps of >20 cM between loci, or regions where loci are missing from chromosome ends, has been reduced from 40 in the previous map to 6. Five hundred and seventy-three of the loci can be ordered on a framework map with odds of >1000 : 1. The sheep linkage map contains strong links to both the cattle and goat maps. Five hundred and seventy-two of the loci positioned on the sheep linkage map have also been mapped by linkage analysis in cattle, and 209 of the loci mapped on the sheep linkage map have also been placed on the goat linkage map. Inspection of ruminant linkage maps indicates that the genomic coverage by the current sheep linkage map is comparable to that of the available cattle maps. The sheep map provides a valuable resource to the international sheep, cattle, and goat gene mapping community.Sheep play an important role in modern agriculture and possess many heritable traits that are of economic importance. Additionally, the size, physiology, temperament, and lifespan of sheep make them an appropriate model for studying a variety of mammalian biological functions including lung physiology, immunology, endocrinology, reproduction, embryology, and fetal development. Sheep are also useful as disease models for inherited diseases, such as asthma (Wright et al.

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“…There are two families of the IF, type I and type II, and are coded for by separate gene loci that have been mapped to chromosomes 11 and 3 respectively [14]. Linkage mapping using the AgResearch IMF flock has confirmed these physical map positions [19].…”

Section: Keratin Genesmentioning

confidence: 97%

Major genes and QTL influencing wool production and quality: a review

Purvis

Franklin

2005

Genet. Sel. Evol.

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-The opportunity exists to utilise our knowledge of major genes that influence the economically important traits in wool sheep. Genes with Mendelian inheritance have been identified for many important traits in wool sheep. Of particular importance are genes influencing pigmentation, wool quality and the keratin proteins, the latter of which are important for the morphology of the wool fibre. Gene mapping studies have identified some chromosomal regions associated with variation in wool quality and production traits. The challenge now is to build on this knowledge base in a cost-effective way to deliver molecular tools that facilitate enhanced genetic improvement programs for wool sheep.gene / major gene / QTL / wool production / wool quality

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Linkage mapping of wool keratin and keratin-associated protein genes in sheep

Cited by 76 publications

References 12 publications

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

An Enhanced Linkage Map of the Sheep Genome Comprising More Than 1000 Loci

Major genes and QTL influencing wool production and quality: a review

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