Linkage between marker genes on barley chromosome 2 and a gene for resistance to Heterodera avenae

Andersen, Sigurd; Andersen, Kirsten

doi:10.1111/j.1601-5223.1973.tb01089.x

Cited by 18 publications

(6 citation statements)

References 6 publications

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“…The seeds of eight barley (Hordeum vulgare) cultivars, Skiff, Schooner, Sloop, Sloop SA (Rha2), Sloop VIC (Rha2), Galleon (Rha4), Chebec (Rha2) and Dash (resistant but not known to carry either Rha2 or Rha4), were provided by Stewart Coventry (University of Adelaide, Australia). In previous work, Kretschmer et al (1997) concluded that the resistance locus in Chebec was Rha2 (Cotten & Hayes, 1969;Andersen & Andersen, 1973). A similar locus was mapped in Sahara 3771.…”

Section: Plant Materialsmentioning

confidence: 81%

“…In previous work, Kretschmer et al . () concluded that the resistance locus in Chebec was Rha2 (Cotten & Hayes, ; Andersen & Andersen, ). A similar locus was mapped in Sahara 3771.…”

Section: Methodsmentioning

confidence: 99%

“…Natural resistance mechanisms to cyst nematodes exist and, in some cases, such as soybean cyst nematode, have been attributed to specific genetic polymorphisms (Liu et al, 2012). In barley, four loci affecting resistance have been genetically mapped: three loci, including Rha1, Rha2 and Rha3, are located on chromosome 2H (Cotten & Hayes, 1969;Andersen & Andersen, 1973;Kretschmer et al, 1997), and one, Rha4, is located on chromosome 5H (Barr et al, 1998). Of these, Rha2 and Rha4 are effective against the H. avenae pathotype in Australia and have been extensively used in barley breeding.…”

Section: Introductionmentioning

confidence: 99%

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The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)‐β‐glucan levels and HvCslF gene transcript abundance

et al. 2015

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SummaryHeterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites. In resistant host plants, relatively few females develop to maturity. Little is known about the physiological and biochemical changes induced during CCN infection.Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation. Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-b-glucan. Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition. During H. avenae infection, (1,3;1,4)-b-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-b-glucan during dicot cyst nematode infections. Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-b-glucan synthesis, suggest a role for these genes in the CCN infection process.

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Section: Plant Materialsmentioning

confidence: 81%

“…In previous work, Kretschmer et al . () concluded that the resistance locus in Chebec was Rha2 (Cotten & Hayes, ; Andersen & Andersen, ). A similar locus was mapped in Sahara 3771.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)‐β‐glucan levels and HvCslF gene transcript abundance

et al. 2015

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“…In barley, the inheritance of CCN resistance was first investigated by Nilsson-Ehle ( 1920 ), who reported results indicating segregation of a single unit of inheritance, for which resistance was dominant. Subsequent research established that there are up to three CCN resistance loci on chromosome 2H (Andersen and Andersen 1968 , 1973 ; Cotten and Hayes 1969 ; Kretschmer et al 1997 ). Of these, two (originally called Ha2 and Ha3 but now called Rha2 and Rha3 ) are very closely linked with each other (or may even be the same locus).…”

Section: Introductionmentioning

confidence: 99%

Fine mapping of Rha2 in barley reveals candidate genes for resistance against cereal cyst nematode

et al. 2019

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Key message The cereal cyst nematode resistance locus Rha2 was mapped to a 978 kbp region on the long arm of barley chromosome 2H. Three candidate genes are discussed. Abstract The cereal cyst nematode (CCN) Heterodera avenae is a soil-borne obligate parasite that can cause severe damage to cereals. This research involved fine mapping of Rha2 , a CCN resistance locus on chromosome 2H of barley. Rha2 was previously mapped relative to restriction fragment length polymorphisms (RFLPs) in two mapping populations. Anchoring of flanking RFLP clone sequences to the barley genome assembly defined an interval of 5077 kbp. Genotyping-by-sequencing of resistant and susceptible materials led to the discovery of potentially useful single nucleotide polymorphisms (SNPs). Assays were designed for these SNPs and applied to mapping populations. This narrowed the region of interest to 3966 kbp. Further fine mapping was pursued by crossing and backcrossing the resistant cultivar Sloop SA to its susceptible ancestor Sloop. Evaluation of F 2 progeny confirmed that the resistance segregates as a single dominant gene. Genotyping of 9003 BC 2 F 2 progeny identified recombinants. Evaluation of recombinant BC 2 F 3 progeny narrowed the region of interest to 978 kbp. Two of the SNPs within this region proved to be diagnostic of CCN resistance across a wide range of barley germplasm. Fluorescence-based and gel-based assays were developed for these SNPs for use in marker-assisted selection. Within the candidate region of the reference genome, there are nine high-confidence predicted genes. Three of these, one that encodes RAR1 (a cysteine- and histidine-rich domain-containing protein), one that is predicted to encode an acetylglutamate kinase and one that is predicted to encode a tonoplast intrinsic protein, are discussed as candidate genes for CCN resistance. Electronic supplementary material The online version of this article (10.1007/s00122-019-03279-3) contains supplementary material, which is available to authorized users.

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“…Especially interesting is the Ha2 ‐gene which confers resistance to H. avenae race 1 and 2 (Andersen and Andersen 1973). The selection of resistant genotypes relies on the count of cysts infesting the roots of plants, a simple but laborious and thus expensive bioassay (Andersen and Andersen 1973). Ha2 ‐linked restriction fragment length polymorphism (RFLP) markers have been developed (Kretschmer et al.…”

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confidence: 99%

Development of an ISSR‐derived PCR marker linked to nematode resistance (Ha2) in spring barley

Dayteg¹,

Rasmussen²,

Tuvesson³

et al. 2007

Plant Breeding

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Cereal cyst nematodes (Heterodera avenae Woll.) are economically damaging barley parasites in most cereal growing areas of the world and the development of resistant cultivars is the best measure against the pathogen. An ISSR (inter-simple sequence repeat) marker identified as closely linked with the H. avenae race 1 and 2 resistance gene (Ha2) has been converted into a codominant sequence characterized amplified region marker (Ha2S18) and mapped in barley ÔSW BuddyÕ · ÔSW CeciliaÕ DH population at 4.3 cM from the Ha2 locus on the long arm of chromosome 2H. The potential usefulness of Ha2S18 in large scale marker assisted selection schemes has been evaluated in a broad genetic background and is an important complement to the bioassay and to other linked DNA-markers for this trait.

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Linkage between marker genes on barley chromosome 2 and a gene for resistance to Heterodera avenae

Cited by 18 publications

References 6 publications

The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)‐β‐glucan levels and HvCslF gene transcript abundance

The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)‐β‐glucan levels and HvCslF gene transcript abundance

Fine mapping of Rha2 in barley reveals candidate genes for resistance against cereal cyst nematode

Development of an ISSR‐derived PCR marker linked to nematode resistance (Ha2) in spring barley

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