Link between microbial composition and carbon substrate-uptake preferences in a PHA-storing community

Albuquerque, Maria G.E.; Carvalho, Gilda; Kragelund, Caroline; Silva, Ana F.; Crespo, Maria Teresa Barreto; Reis, Maria A.M.; Nielsen, Per Halkjær

doi:10.1038/ismej.2012.74

Cited by 128 publications

(86 citation statements)

References 39 publications

(43 reference statements)

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“…The impact of pH, OLR, and substrate consumption preferences was investigated by Oehmen et al (2013); Carvalho et al (2014), and Albuquerque et al (2013), respectively, using a MMC acclimatized to fermented molasses dominated by Azoarcus (63.2 %) and Thauera (25.1 %), as described by Albuquerque et al (2010). In these three works, the authors imposed cycles of 12 h, a HRT of 1 day, and a SRT of 10 days.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

“…This technique also permits the identification of the microorganisms involved (Lee et al 1999), but it means an increase in the technique complexity. During the SBR cycles, Albuquerque et al (2013), using FISH, observed that 84 % of the selected MMC was constituted by Paracoccus (50 %), Azoarcus, (26 %) and Thauera (8 %). Despite different uptake rates being obtained when each carbon source was fed individually and mixed with other VFAs, MAR-FISH showed the preference for each VFA by these genera.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

“…Extensive studies have been done on the process engineering to understand the impact of the different SBR-operating conditions on PHA production rates, yields, and final composition (Dionisi et al 2007;Serafim et al 2008b;Villano et al 2010a;Moralejo-Gárate et al 2013;Laycock et al 2014;MontanoHerrera et al 2014;Valentino et al 2014). However, ecological understanding has been consigned to a second plan hindering the development of strategies that cover high selective pressure and high biomass production rate (Reis et al 2011;Albuquerque et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Unveiling PHA-storing populations using molecular methods

Queirós

Lemos

Rossetti

et al. 2015

Appl Microbiol Biotechnol

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Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process.

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Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Unveiling PHA-storing populations using molecular methods

Queirós

Lemos

Rossetti

et al. 2015

Appl Microbiol Biotechnol

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“…Este resultado faz com que a torta de tabaco possa ser utilizada como fonte de carbono para obtenção de biopolímeros como o P(3HB) produzido por microrganismos (ALBUQUERQUE et al, 2011;ALBUQUERQUE et al, 2013;REDDY et al, 2003;HAYWOOD et al, 1990;RAMACHANDRAN et al, 2007).…”

Section: Composição Centesimal Da Torta De Tabacounclassified

Aproveitamento de torta do tabaco gerado na produção de biodiesel para a obtenção de bioplástico

Riça

Moraes

Souza

et al. 2016

RJP

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O poli 3-hidroxibutirato – P(3HB) – é o principal polímero da classe dos poli hidroxialcanoatos (PHAs), polímeros biodegradáveis sintetizados por microrganismos a partir de diversos substratos. O objetivo desse trabalho foi a produção de P(3HB) a partir do resíduo da extração do óleo da semente de tabaco (Nicotiana tabacum L.) como substrato alternativo e de baixo custo para triagem de microrganismos produtores de P(3HB). O resíduo foi avaliado quanto a sua composição e beneficiado através de hidrólise. O hidrolisado foi utilizado em ensaio de fermentação com cepas bacterianas isoladas da Estação de Tratamento de Efluentes (ETE) da Universidade de Santa Cruz do Sul (UNISC). Para visualização de inclusões lipofílicas nas células, indicadoras de acúmulo de P(3HB), utilizou-se coloração de Sudan Black. A extração do polímero do interior das células foi realizada utilizando-se clorofórmio e hipoclorito de sódio e o produto extraído foi analisado em espectrofotômetro. De 25 isolados, a cepa E10 foi a que apresentou melhor resultado frente à coloração de Sudan Black. A análise do produto obtido sugere que a E10 produziu PHAs utilizando o resíduo de tabaco, demonstrando seu potencial como substrato de baixo custo para crescimento microbiano e obtenção de biopolímeros.

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“…A study carried out by Albuquerque et al (2013) has shown that even when using the same profile of VFA feeding, in experiments operated under the same conditions, differences in 3HV content in the final PHBV copolymer have been observed. This phenomenon could be associated with differences in the community structure.…”

Section: Pha Synthesismentioning

confidence: 99%

Composition of mixed culture PHA biopolymers and implications for downstream processing

Montano-Herrera¹

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Polyhydroxyalkanoates (PHAs) are biopolymers synthesised and accumulated by most bacteria for carbon and energy storage. They have properties and applications comparable to petrochemical thermoplastics. Although PHAs are produced at high yields using pure biological cultures, the use of mixed cultures can significantly reduce the production costs and make use of waste streams to produce environmentally sustainable materials. In order to produce mixed culture PHAs of relevance for broader industry applications it is necessary to develop the ability to tailor polymers with diversified mechanical properties through understanding and controlling the monomer composition and compositional distribution.Diverse and complex PHA polymer structures have been achieved in mixed microbial cultures using time-based feeding strategies. However, PHA monomer composition and compositional distribution in PHA random and blocky copolymers are sensitive to substrate feeding history, making more complex the prediction of final PHA content and composition.In this sense, a better understanding of the changing cell physiologies that develop in response to different feeding strategies and substrates is necessary to design optimised feeding strategies and process control algorithms for PHA production.This thesis presents for the first time a comprehensive flux characterisation of monomer development during mixed culture PHA accumulation concurrent with biomass growth using Metabolic Flux Analysis (MFA). A dynamic trend in active biomass growth and in polymer composition was observed and was consistent over replicate accumulations. Monomer (3-hydroxybutyrate and 3-hydroxyvalerate) incorporation into poly(3-hydroxybutyate-co-3-hydroxyvalerate) copolymers in a pilot scale production system was evaluated based on published models that describe polymer production during PHA accumulation. However, it was found that these existing models could not describe the fluctuations in the proportion of Additionally, thermal degradation of mixed culture PHAs during melt processing was assessed by Near-Infrared (NIR) spectroscopy coupled to Multivariate Data Analysis. It was shown that, with correct pretreatment, a copolymeric product that was much more stable to extrusion processing than commercially available PHA was produced.Overall, this thesis explores both the fundamental and applied aspects of PHA production by mixed microbial cultures with concurrent active biomass growth. The use of computational iii tools to explore and help understand the underlying metabolic processes was explored.Polymer composition seems to follow a very complex regulation processes which can be described through the incorporation of more detailed reactions in current metabolic models.Furthermore, this thesis gives further insight into the fundamental properties of the materials produced and an assessment of their potential for degradation during processing.iv

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Link between microbial composition and carbon substrate-uptake preferences in a PHA-storing community

Cited by 128 publications

References 39 publications

Unveiling PHA-storing populations using molecular methods

Unveiling PHA-storing populations using molecular methods

Aproveitamento de torta do tabaco gerado na produção de biodiesel para a obtenção de bioplástico

Composition of mixed culture PHA biopolymers and implications for downstream processing

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