Lining of viable and nonviable allogeneic and xenogeneic cardiovascular tissue with cultured adult human venous endothelium

Bengtsson, Lars; Ragnarson, Birger; Hægerstrand, Anders

doi:10.1016/s0022-5223(19)34076-0

Cited by 20 publications

(10 citation statements)

References 32 publications

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“…From commercially available collagen-or gelatin-coated vascular grafts, formaldehyde or glutaraldehyde residues are released. Therefore, these commercially available grafts will not support the proliferation of seeded endothelial cells (14,16,30).…”

Section: Discussionmentioning

confidence: 99%

“…Of these crosslinking agents, which are incorporated in the protein coating during crosslinking (12), notably glutaraldehyde is known to evoke cytotoxic reactions in vitro as well as in vivo (13)(14)(15)(16). Upon implantation, cytotoxicity hampers endothelialization of currently available collagencoated graft materials (14,17). As a consequence there is a need to develop a non-cytotoxic crosslinked coating of collagen, which supports adherence and proliferation of seeded endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

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Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Wissink

Luyn

Beernink³

et al. 2000

Thromb Haemost

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SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Wissink

Luyn

Beernink³

et al. 2000

Thromb Haemost

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“…The subendothelium of the sheep carotid artery that is exposed following a minimum of 4 weeks of cold storage may retain some adhesive properties. If binding sites for ECs are present, it may be possible to reendothelialize the surface through the technique of seeding using ECs or stem cells isolated from blood vessels and fat (3,38). Following seeding, the ECs might be capable of producing a new, more complete basement membrane on which to spread and replicate (3,17,19,21).…”

Section: Discussionmentioning

confidence: 99%

“…If binding sites for ECs are present, it may be possible to reendo-Cellular changes: Following 1 and 2 weeks of cold storthelialize the surface through the technique of seeding age, many ECs were necrotic and in the process of using ECs or stem cells isolated from blood vessels and detaching. Some of the SMCs and fibroblasts were fat (3,38). Following seeding, the ECs might be capable also necrotic.…”

Section: Histology Materials and Methodsmentioning

confidence: 99%

Morphological Changes in the Ovine Carotid Artery Wall Induced by Cold Storage

Smardencas

Birchall

2011

Cell Transplant

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Blood vessels obtained from cadavers and amputated limbs stored at 4°C (i.e., cold stored) potentially represent an economical and readily sourced alternative to autologous vessels and synthetic prostheses for vascular reconstructive surgery. However, cold-stored vessels would need to have a reduced antigenicity and an antithrombogenic autologous endothelial cell (EC) lining before they could function as patent vascular allografts. The aim of this study was to determine the effect of cold storage for 1-16 weeks on the morphology of the ovine carotid artery wall. Ovine carotid arteries (n = 6) were rinsed and flushed with 0.9% saline, cut into segments, wrapped in 0.9% saline-soaked gauze, and stored at 4°C for 1, 2, 4, 8, or 16 weeks. Following storage, the segments were sampled and the samples fixed and sectioned for light microscopic, immunohistochemical, or transmission electron microscopic examination. After 1 and 2 weeks the ECs were karyolitic or contained pyknotic nuclei. After 4 weeks the EC layer was depleted, the subendothelial matrix exposed, and the number of smooth muscle cells (SMCs) and fibroblasts reduced. The 8-and 16-week samples demonstrated complete loss of the EC lining and only occasional remnants of SMCs or fibroblasts. The subendothelial basement membrane appeared to undergo degradative changes as early as 1 week following cold storage. At each time point examined, the subendothelial connective tissue stroma, the internal elastic lamina (IEL), and the collagenous and elastic extracellular framework were retained. These results demonstrate that the ovine carotid artery wall progressively loses its cells but retains its extracellular components during cold storage for up to 16 weeks. They suggest that cold-stored vessels may function as allografts with a reduced antigenicity for vascular reconstructive surgery. It is conceivable that seeded autologous ECs may be used to restore the antithrombogenic EC lining prior to graft implantation.

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“…Coating these materials with proteins that promote endothelial cell adhesion and migration may improve seeding onto these materials. Indeed, there are commercially available synthetic grafts with albumin, gelatin, and collagen coatings, but these proteins are cross‐linked with glutaraldehyde or formaldehyde and thus have a low level of cytotoxicity which inhibits endothelialization (10,11). Furthermore, the seeding of vascular grafts with autologous endothelial cells which have been expanded in vitro to obtain sufficient numbers of cells for complete luminal coverage of a graft is not practical at this time, and therefore preoperative seeding at lower densities may be used.…”

mentioning

confidence: 99%

Basic Fibroblast Growth Factor Coating and Endothelial Cell Seeding of a Decellularized Heparin‐coated Vascular Graft

Conklin

Lin

et al. 2004

Artificial Organs

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The objective of this study was to determine the effect of basic fibroblast growth factor (bFGF) coating on endothelial cell seeding and proliferation on a decellularized heparin coated vascular graft and to determine the retention of seeded cells on the graft under flow conditions. Disks of heparin coated decellularized grafts were incubated for 24 h as controls or with bFGF. Human microvascular endothelial cells (HMECs) or canine peripheral blood endothelial progenitor cells (CEPC) were seeded onto the disks and incubated for 96 h or 48 h, respectively. HMECs were also seeded onto the luminal surfaces of two heparin-coated decellularized grafts for 3 h. One graft was placed in a perfusion culture system and cultured for an additional 6 h with flow and pressure. After culturing, there were 4.7 +/- 1.4 cells/mm(2) HMECs on control grafts and 11.4 +/- 1.4 cells/mm(2) in bFGF treated grafts (P < 0.05). Likewise, with CEPCs, there were 14.8 +/- 4.8 cells/mm(2) in control grafts and 33.3 +/- 7.3 cells/mm(2) in bFGF treated grafts. After only 3 h of cell attachment, 60% of HMECs were retained in the intact graft exposed flow relative to the static control graft, which is an acceptable level. These data demonstrate that bFGF coating on the heparin bound decellularized grafts significantly increases both HMEC and dog EPC proliferation and that seeded cells are stable under perfusion conditions.

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Lining of viable and nonviable allogeneic and xenogeneic cardiovascular tissue with cultured adult human venous endothelium

Cited by 20 publications

References 32 publications

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

Morphological Changes in the Ovine Carotid Artery Wall Induced by Cold Storage

Basic Fibroblast Growth Factor Coating and Endothelial Cell Seeding of a Decellularized Heparin‐coated Vascular Graft

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