Linezolid-resistant clinical isolates of enterococci and Staphylococcus cohnii from a multicentre study in China: molecular epidemiology and resistance mechanisms

Chen, Hongbin; Wu, Weiyuan; Ni, Ming; Liu, Yingmei; Zhang, Jixia; Xia, Fei; He, Wenqiang; Wang, Qi; Wang, Zhanwei; Cao, Bin; Wang, Hui

doi:10.1016/j.ijantimicag.2013.06.008

Cited by 59 publications

(46 citation statements)

References 29 publications

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“…In addition, the plasmids pRM01 (accession no. KC820815) and pHS, which were detected in clinical S cohnii and S. aureus isolates from tertiary hospitals in China, were also similar to plasmid pSS-01 with respect to the genetic Community-onset cfr-positive S. aureus infection in China environment of the cfr gene (Tian et al, 2014;Chen et al, 2013). In 2012, a clinical Staphylococcus epidermidis from the USA was reported to have a similar plasmid, p7LC (accession no.…”

Section: Discussionmentioning

confidence: 93%

“…So far, linezolid-resistant staphylococci and enterococci isolates from tertiary hospitals in China have been described (Cai et al, 2012;Tian et al, 2014;Chen et al, 2013;Cui et al, 2013). In China, the cfr gene is widely distributed in isolates of human and animal origin, particularly coagulase-negative staphylococcus isolates (Cai et al, 2012;Tian et al, 2014;Chen et al, 2013;Cui et al, 2013;Wang et al, 2013). This study identified 14 S. aureus isolates that harbour the cfr gene; this is, we believe, the first time that the cfr gene has been reported in community-onset S. aureus in China.…”

Section: Discussionmentioning

confidence: 99%

“…This gene was first identified in a bovine Staphylococcus sciuri isolate (Schwarz et al, 2000), and it was subsequently detected in clinical staphylococci isolates from different countries (Mendes et al, 2008(Mendes et al, , 2010aBongiorno et al, 2010;Sánchez García et al 2010). More recently, some investigators in China reported the presence of the cfr gene in clinical staphylococci and enterococci (Cai et al, 2012;Tian et al, 2014;Chen et al, 2013;Cui et al, 2013). The current study investigated the resistance phenotype and resistance mechanism of 14 cfr-positive S. aureus isolates from community-acquired infections, and the genetic environment and potential transmission mechanism of cfr were further analysed.…”

Section: Introductionmentioning

confidence: 89%

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Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China

Zhao

Zheng

et al. 2015

Journal of Medical Microbiology

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The cfr gene was detected in 14 meticillin-susceptible Staphylococcus aureus isolates recovered from outpatients with community-onset infections in a county hospital in China. The MIC of linezolid was 4 mg ml 21 in eight isolates and 2 mg ml 21 in six isolates. All isolates were susceptible to vancomycin and teicoplanin, but had elevated MICs for penicillin (0.5-128 mg ml 21), chloramphenicol (2-32 mg ml 21), clindamycin (0.5-128 mg ml 21) and erythromycin (4-128 mg ml 21 ). Nine isolates had mutations on domain V of 23S rRNA and/or the ribosomal L proteins that were not located close to the linezolid-binding pocket. Southern blotting experiments demonstrated that the cfr genes in all 14 isolates resided on plasmids. Sequence analysis of the 5.6 kb cfr-carrying plasmid segment revealed 99 % identity to the corresponding sequences in plasmid pSS-01 from animal staphylococci and plasmid pRM-01 from human staphylococci. Five isolates belonged to sequence type (ST)188 and three to ST965; the two ST types were previously reported in isolates of animal origin in some areas of China. These results indicate that the cfr-carrying plasmids in this study are likely of animal origin. The present study shows that cfr-harbouring S. aureus isolates have emerged in some areas of China and that cfr-carrying isolates may be transmitted between animals and humans.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

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Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China

Zhao

Zheng

et al. 2015

Journal of Medical Microbiology

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“…In another study, linezolid nonsusceptibility among staphylococci was associated with the G2576T mutation in 23S rDNA, whereas 5 isolates carried the mobile cfr element [15]. Additionally, the presence of the G2576T mutation in 23S rDNA (mutations in L3 or L4 genes) resulted in reduced susceptibility to linezolid among enterococci [16,17]. …”

Section: Discussionmentioning

confidence: 99%

Factors Influencing Linezolid-Nonsusceptible Coagulase-Negative Staphylococci Dissemination Among Patients in the Intensive Care Unit: A Retrospective Cohort Study

Papadimitriou-Olivgeris

Giormezis

Fligou³

et al. 2013

Chemotherapy

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Background: The aim of the present study was to identify risk factors for linezolid-nonsusceptible coagulase-negative staphylococci (CNS) dissemination in the intensive care unit. Methods: Among the 246 patients included, 33 revealed a linezolid-nonsusceptible CNS-positive culture specimen, 68 were positive for linezolid-susceptible CNS and 145 served as controls. Isolates were characterized by phenotypic and genotypic methods to species level, susceptibility to antistaphylococcal agents and clones. Results: Among the 33 linezolid-nonsusceptible CNS patients, 29 revealed Staphylococcus epidermidis and 4 Staphylococcus capitis. All S. epidermidis strains belonged to the ST22 clone (by multilocus sequence typing), 26 carried both C2534T and T2504A and 3 strains were C2543T mutations. S. capitis strains were stratified as a common pulsed-field gel electrophoresis type and carried the G2576T mutation. Risk factors for linezolid-nonsusceptible CNS isolation were linezolid administration and mean number of linezolid-nonsusceptible CNS-positive patients in nearby beds per day. Conclusions: These results reinforce the aspect of rational antibiotic usage, but also highlight the need for strict infection control measures to prevent the dissemination of linezolid-nonsusceptible CNS.

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“…Soon, linezolid resistance in human clinical CoNS seems to have become an increasing problem in China. Several linezolid-resistant clinical isolates of Staphylococcus capitis (n ϭ 9), Staphylococcus cohnii (n ϭ 6), Staphylococcus haemolyticus (n ϭ 1), Staphylococcus epidermidis (n ϭ 1), and Staphylococcus hominis (n ϭ 1) from Shenyang (n ϭ 3), Beijing (n ϭ 3), Hangzhou (n ϭ 10), Rui'an (n ϭ 1), and Nanjing (n ϭ 1) have been reported (8)(9)(10)(11). To date, there has been no report of linezolid-resistant MRSA (LRSA) of human origin in China.…”

mentioning

confidence: 99%

Dissemination of the Same cfr -Carrying Plasmid among Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococcal Isolates in China

Cai

Hong

et al. 2015

Antimicrob Agents Chemother

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Six cfr-harboring methicillin-resistant Staphylococcus aureus (MRSA) isolates, which belonged to the same clone of sequence type 5 (ST5)-staphylococcal cassette chromosome mec element II (SCCmec II)-spa t311, were investigated in this study. Complete sequencing of a cfr-carrying plasmid, pLRSA417, revealed an 8,487-bp fragment containing a Tn4001-like transposon, cfr, orf1, and ISEnfa4. This segment, first identified in an animal plasmid, pSS-01, was observed in several plasmids from clinical coagulase-negative staphylococci in China, suggesting that the cfr gene, which might originate from livestock, was located in the same mobile element and disseminated among different clinical staphylococcal species.

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Linezolid-resistant clinical isolates of enterococci and Staphylococcus cohnii from a multicentre study in China: molecular epidemiology and resistance mechanisms

Cited by 59 publications

References 29 publications

Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China

Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China

Factors Influencing Linezolid-Nonsusceptible Coagulase-Negative Staphylococci Dissemination Among Patients in the Intensive Care Unit: A Retrospective Cohort Study

Dissemination of the Same cfr -Carrying Plasmid among Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococcal Isolates in China

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