Linear sweep voltammetric studies on the supramolecular complex of alizarin red S with lysozyme and determination of lysozyme

Abstract

An electrochemical method for the determination of lysozyme (LYS) based on its interaction with alizarin red S (ARS) was established by linear sweep voltammetry in this paper. The electrochemical behaviour of ARS with LYS was investigated on a dropping mercury working electrode in 0⋅2 mol/L pH 4⋅8 Britton-Robinson (B-R) buffer solution. ARS showed a sensitive second order derivative linear sweep voltammetric reductive peak at -0⋅42 V (vs SCE). After the addition of LYS, the reductive peak current of ARS decreased without the shift of the reductive peak potential and no new waves appeared, which was due to the formation of a supramolecular complex of ARS with LYS in the solution. The stoichiometry of the ARS-LYS complex was further calculated by the electrochemical data with the results of the binding ratio as 3 : 1 and the binding constant as 2⋅82 × 10 14 . Under the selected conditions, the decrease of the second order derivative linear sweep voltammetric reductive peak current of ARS was in proportion to the LYS concentration in the range from 0⋅8 to 35⋅0 mg/L and the detection limit of LYS was calculated as 0⋅52 mg/L (3σ). Different kinds of LYS samples were detected satisfactorily with this method.

A Novel Electrochemical Method for Protionamide Determination Based on Its Interaction with Alizarin Red S

“…A colorimetric determination method for dothiepin hydrochloride in pharmaceutical formulations with alizarin red S as a chromogenic agent was established by Abdulrahman and Basavaiah [9]. We also developed a new method for lysozyme determination using alizarin red S as electrochemical probe by linear sweep voltammetry [10].…”

Electrocatalytic Oxidation of NADH Using Alizarin Immobilized Carbon Nanotube Modified Electrode

“…It is a histochemical stain that highlights calcium, vastly utilized by dermatopathologists to detect dermal calcium in disorders such as pseudoxanthoma elasticum and calcinosis cutis [5]. AZ widely used as a mediator or complexing agent to detect various analytes and metal ions, for instance, hydrazine [6], nitrite [7], proteins like lysozyme [8] and ultra trace copper [9]. In past, AZ was paid more attention in the electroanlaytical chemistry in preparing electro-active films which are suitable for electrocatalytic oxidation/reduction of certain biological compounds.…”