Linear relationship between lethal mutation yield and intake of ethyl methanesulfonate in <i>Drosophila melanogaster</i>

Ayaki, Toshikazu; Oshima, Kayoko; Yoshikawa, Isao

doi:10.1002/em.2860070203

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…We measured the water consumption of flies fed two different concentrations (dietary restriction, DR; concentrated medium, CM) of yeast extract/sucrose (YE/S) medium by providing ad libitum water labeled with a radioactive tracer (21)(22)(23). Flies were housed in population cages containing separate food and water sources of similar surface area.…”

Section: Resultsmentioning

confidence: 99%

Water- and nutrient-dependent effects of dietary restriction on Drosophila lifespan

Carvalho

Zid

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

146

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Dietary restriction (DR) is a widely conserved intervention leading to lifespan extension. Despite considerable effort, the mechanisms underlying DR remain poorly understood. In particular, it remains unclear whether DR prolongs life through conserved mechanisms in different species. Here, we show that, in the most common experimental conditions, lifespan extension by DR is abolished by providing Drosophila with ad libitum water, without altering food intake, indicating that DR, as conventionally studied in flies, is fundamentally different from the phenomenon studied in mammals. We characterize an alternative dietary paradigm that elicits robust lifespan extension irrespective of water availability, and thus likely represents a more relevant model for mammalian DR. Our results support the view that protein:carbohydrate ratio is the main dietary determinant of fly lifespan. These findings have broad implications for the study of lifespan and nutrition.aging ͉ caloric restriction ͉ dehydration ͉ longevity ͉ nutrition D ietary restriction (DR), classically defined as a reduction in nutrient availability short of malnutrition, can extend the lifespan of organisms ranging from yeast to mice (1, 2). In rodents and primates, DR delays the onset of age-related pathologies, such as cancer, cardiovascular disease, and diabetes (3-5). Chronic DR also elicits a number of physiological changes, including decreased circulating glucose, insulin, and cholesterol levels; reduced body mass; and compromised reproductive function (5-8). Despite the evident biomedical interest in DR, its mechanistic basis remains largely unknown, and it is unclear whether DR extends lifespan in different species through similar mechanisms (9, 10). This issue is of fundamental importance, since invertebrate model systems are especially valued for their ability to provide mechanistic clues to be tested in mammals.In mammals, food restriction is imposed by feeding the DR cohort a fraction of that ingested by the ad libitum group (2). Due to the difficulty of controlling feeding rates in invertebrates, more ingenious, albeit potentially problematic, techniques are used. Drosophila DR is commonly achieved by total food dilution (11) and carried out in the absence of a separate water source, unlike with other species (12-16). Hence, fly food is simultaneously the source of nutrients* and water. This setup prevents flies from independently regulating nutrient and water intake, leaving room for the possibility that any effects of food dilution are mediated by changes in hydration.Our results show that lifespan extension by typical DR regimes (17-20) can be entirely abolished by providing flies with free access to water. Water supplementation does not affect food consumption, suggesting that DR, as typically applied, does not impact longevity through reduced nutrient intake. Furthermore, we characterize a regime that elicits robust lifespan extension independent of water supplementation. Our findings suggest that most of the work done on Drosophila DR has b...

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Section: Resultsmentioning

confidence: 99%

Water- and nutrient-dependent effects of dietary restriction on Drosophila lifespan

Carvalho

Zid

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

146

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show abstract

“…Labeling strategies, where dyes 10,11 or radioactive tracers 1,4,12–18 are added to the medium, are frequently used for measuring fly feeding, but have several disadvantages: a) they involve disturbing the animals upon transfer onto the labeled medium b) the amount of label in the fly is only informative during the linear phase of accumulation 19 and c) animals must be sacrificed for each measurement, making it impossible to continuously monitor individual animals. Alternatively, feeding has been inferred from either the number of flies standing on the food 20 or the fraction of flies observed eating (via proboscis extension) 9,21 .…”

Section: Introductionmentioning

confidence: 99%

“…Here, we determine the capacity of four commonly used adult Drosophila feeding assays—the CAFE 22 , food-labeling with radioactive tracers 1,4,12–17 or dyes 10,11 , and PE 9,21 —to resolve differences in consumption. Our results suggest that the CAFE and radioisotope-labeling provide the most consistent and accurate results and the highest resolving power over the widest range of conditions.…”

Section: Introductionmentioning

confidence: 99%

Quantifying Drosophila food intake: comparative analysis of current methodology

et al. 2014

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Food intake is a fundamental parameter in animal studies. Despite the prevalent use of Drosophila in laboratory research, precise measurements of food intake remain challenging in this model organism. Here, we compare several common Drosophila feeding assays: the Capillary Feeder (CAFE), food-labeling with a radioactive tracer or a colorimetric dye, and observations of proboscis extension (PE). We show that the CAFE and radioisotope-labeling provide the most consistent results, have the highest sensitivity, and can resolve differences in feeding that dye-labeling and PE fail to distinguish. We conclude that performing the radiolabeling and CAFE assays in parallel is currently the best approach for quantifying Drosophila food intake. Understanding the strengths and limitations of food intake methodology will greatly advance Drosophila studies of nutrition, behavior, and disease.

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Dosage-response relationships for methyl methanesulfonate in Drosophila melanogaster spermatozoa: DNA methylation per nucleotide vs. sex-linked recessive lethal frequency

Lee¹,

1989

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Linear relationship between lethal mutation yield and intake of ethyl methanesulfonate in Drosophila melanogaster

Cited by 3 publications

References 14 publications

Water- and nutrient-dependent effects of dietary restriction on Drosophila lifespan

Water- and nutrient-dependent effects of dietary restriction on Drosophila lifespan

Quantifying Drosophila food intake: comparative analysis of current methodology

Dosage-response relationships for methyl methanesulfonate in Drosophila melanogaster spermatozoa: DNA methylation per nucleotide vs. sex-linked recessive lethal frequency

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