Linear molecular beacons for highly sensitive bioanalysis based on cyclic Exo III enzymatic amplification

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

“…4 B). To reduce background signals and improve hybridization dynamics, linear molecular beacons (LMB) were used to label the fluorophores and quenchants on the terminal and penultimate nucleotides, respectively [ 48 ]. In their study, the padlock probes as the circular template were ligated and circularized by the ssDNA ligase.…”

Recent Progress in Aptamer‐Based Functional Probes for Bioanalysis and Biomedicine

“…In our group, the CEAMw as first extended for the detection of as mall molecule, cocaine, using its corresponding aptamer. [70] In the presenceo fc ocaine, the designed aptamer fragment and the linear molecular beacon (LMB), which has af luorophore andq uencher attached to 3'-terminal and penultimate nucleotides, respectively,i nteracted with the target and assembled into an aptamer-target complex. This resulted in formation of ab lunt 3'-terminus duplexD NA, which was digested by Exo III to releaset he free fluorophore for signaling and the target for the next reactionc ycle, thus generating significant fluorescent signal amplification.…”

Ultrasensitive electrochemical detection of BCR/ABL fusion gene fragment based on polymerase assisted multiplication coupling with quantum dot tagging

“…To solve this problem, signal amplification strategies were considered. Signal amplification can be achieved by two main methods: loading massive signal labels such as enzymes or quantum dots (QDs) [10,11], and cycling target with the aid of nucleases including endonuclease, polymerase, and exonuclease [12][13][14]. Among the target DNA cycling methods, circular strand replacement polymerization (CSRP), which was assisted by polymerase, has attracted considerable attention in duplicating target by simple procedures without the need of specific recognition site [15,16].…”