Ultrasensitive electrochemical detection of BCR/ABL fusion gene fragment based on polymerase assisted multiplication coupling with quantum dot tagging

Hu, Lihui; Tan, Tingting; Chen, Gang; Zhang, Kui; Zhu, Jun‐Jie

doi:10.1016/j.elecom.2013.08.004

Cited by 18 publications

(5 citation statements)

References 19 publications

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“…The LOD of our method is higher than some of the earlier electrochemical fusion gene assays . Although some other studies have reported similar LODs (Table S2) the usage of these methods is hindered mainly because they involve enzyme‐based amplification and complicated surface functionalization steps (the extra steps involved in the immobilization and hybridization of DNA probes on electrodes for capturing target DNA) , or both of them . It is also important to note that only a few electrochemical methods can offer relatively higher sensitivity .…”

Section: Resultsmentioning

confidence: 70%

Detection of FGFR2 : FAM76A Fusion Gene in Circulating Tumor RNA Based on Catalytic Signal Amplification of Graphene Oxide‐loaded Magnetic Nanoparticles

et al. 2018

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Circulating tumor nucleic acids (ctNAs) are promising biomarkers for minimally invasive cancer assessment. The FGFR2 : FAM76A fusion gene is one of the highly promising ovarian cancer biomarkers detectable in ctNAs. Herein, we introduce a new amplification‐free electrochemical assay for the detection of FGFR2 : FAM76A fusion gene in ctNAs extracted from ovarian cancer patients. The assay relies on the electrocatalytic activity of a new class of superparamagnetic graphene‐loaded iron oxide nanoparticles (GO‐NPFe2O3). After isolation and purification, the target RNA was directly adsorbed onto the GO‐NPFe2O3 surface through graphene‐RNA affinity interaction. The electrocatalytic signal was achieved by the reduction of surface‐attached ruthenium hexaammine(III) chloride which was further amplified by using the ferricyanide redox system. Our assay depicted an excellent detection sensitivity down to 1.0 fM, high specificity and excellent reproducibility (% RSD=<5 %, for n=3). The analytical performance of our method was validated with standard qRT‐PCR analysis. We believe that this newly developed assay would be practically applicable in clinical research.

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Section: Resultsmentioning

confidence: 70%

Detection of FGFR2 : FAM76A Fusion Gene in Circulating Tumor RNA Based on Catalytic Signal Amplification of Graphene Oxide‐loaded Magnetic Nanoparticles

et al. 2018

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“…In general, the signal output due to the binding event is limited by the surface density of the hybridization probes, which often imposes a limit on the detection capability. As a consequence, there are several strategies to enhance the obtained signal for the selective detection of DNA, including the use of nanomaterial assemblies [31], the addition of nanotags, such as silver nanoparticles, to the DNA duplex [32], nuclease-assisted target recycling [33], polymerase-based target recycling amplification [34], and isothermal solid-phase amplification [35]. Gold and silver [37] Amperometry n/a H 2 O 2 Paper Graphene [38] Square-wave voltammetry…”

Section: Biosensormentioning

confidence: 99%

Inkjet Printing for Biosensors and Bioelectronics

Adly

Rinklin

2019

Organic Bioelectronics for Life Science and Healthcare

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This chapter examines the role of inkjet printing for the fabrication of biosensors and bioelectronic devices. Inkjet printing is a promising technique that can be employed to directly fabricate sensors and electronics on a variety of substrates including flexible plastics and soft silicones. In contrast to state-of-the-art mask-based microfabrication it is capable of testing new materials and designs in a rapid prototyping approach. As such, it has the potential to play a powerful role in the development of customized devices. Here, we present an overview of recent research describing the application of inkjet technologies to print functional biosensors and bioelectronic devices. We discuss advantages and limitations of this approach and address possible routes to overcome current challenges for future developments in this field.

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“…These methods are sensitivity limited without effective signal amplification strategy. More recently, polymerase coupling with quantum dot tagging is used for ultrasensitive detection of bcr/abl [23]. But this method is enzyme dependent with complex procedures.…”

Section: Introductionmentioning

confidence: 99%

Coupling a universal DNA circuit with graphene sheets/polyaniline/AuNPs nanocomposites for the detection of BCR/ABL fusion gene

Chen

Wang

Sheng

et al. 2015

Analytica Chimica Acta

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Ultrasensitive electrochemical detection of BCR/ABL fusion gene fragment based on polymerase assisted multiplication coupling with quantum dot tagging

Cited by 18 publications

References 19 publications

Detection of FGFR2 : FAM76A Fusion Gene in Circulating Tumor RNA Based on Catalytic Signal Amplification of Graphene Oxide‐loaded Magnetic Nanoparticles

Detection of FGFR2 : FAM76A Fusion Gene in Circulating Tumor RNA Based on Catalytic Signal Amplification of Graphene Oxide‐loaded Magnetic Nanoparticles

Inkjet Printing for Biosensors and Bioelectronics

Coupling a universal DNA circuit with graphene sheets/polyaniline/AuNPs nanocomposites for the detection of BCR/ABL fusion gene

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