LINC00941 promotes oral squamous cell carcinoma progression via activating CAPRIN2 and canonical WNT/β‐catenin signaling pathway

Ai, Yilong; Wu, Si-Pei; Zou, Chen; Wei, Haigang

doi:10.1111/jcmm.15667

Cited by 32 publications

(34 citation statements)

References 45 publications

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“…Some studies have shown that it can promote the growth and metastasis of many tumors. In oral squamous cell carcinoma, the Wnt/β-catenin signal transduction pathway was activated by highly expressed LINC00941; 22 the up-regulation of LINC00941 can accelerate the development of hepatocellular carcinoma by promoting epithelial-mesenchymal transition (EMT) and malignancy. 23 LINC00941 was highly expressed in gastric cancer, which was related to its biological processes.…”

Section: Introductionmentioning

confidence: 99%

Novel lncRNA LINC00941 Promotes Proliferation and Invasion of Colon Cancer Through Activation of MYC

Chang¹,

Zhou²,

Luo³

2021

OTT

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Purpose We conducted the study to elucidate how LncRNA LINC00941 affects colon cancer progression and its possible regulatory mechanism. Methods The expression level of LINC00941 in colon cancer tissues and cells was detected by qRT-PCR. The function of LINC00941 on colon cancer cell proliferation, migration, and invasion was detected by CCK-8 and Transwell assay respectively. The target interactions among LINC00941, miR-205-5p, and MYC were further confirmed by dual-luciferase reporter gene assays and RNA pull-down experiments. Meanwhile, in vivo experiments were carried out to study the role of LINC00941 in the xenotransplantation model. Results LINC00941 expression level was elevated in colon cancer tissues and cells. LINC00941 overexpression accelerated proliferation, migration, and invasion of colon cancer cells, while the LINC00941 knockdown showed the opposite results. In addition, LINC00941 regulated the expression of MYC by sponging miR-205-5p as a competitive endogenous RNA, and miR-205-5p knockdown reversed the tumor inhibition of LINC00941 knockdown on colon cancer cells. Xenograft model assay confirmed that LINC00941 silencing could inhibit colon cancer cell growth and metastasis. Conclusion LINC00941 may markedly promote colon cancer progression by acting on the miR-205-5p/MYC axis as a ceRNA, which offers novel clues for lncRNA to guide the treatment and prognosis of colon cancer.

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Section: Introductionmentioning

confidence: 99%

Novel lncRNA LINC00941 Promotes Proliferation and Invasion of Colon Cancer Through Activation of MYC

Chang¹,

Zhou²,

Luo³

2021

OTT

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“…Some studies suggest that CAPRIN2 has roles in the inhibition of cell growth, differentiation, the enhancement of classical WNT signaling and the maintenance of dendritic structure [ 36 ]. CAPRIN2 is considered to be an oncoprotein in hepatoblastoma [ 37 ] and can induce the development of oral squamous cell carcinoma via activating the WNT/β-catenin signaling pathway [ 38 ]. In the present research, we displayed for the first time that CAPRIN2 was significantly upregulated in CRC cells, which was consistent with the results of the tissue level on the online dataset.…”

Section: Discussionmentioning

confidence: 99%

The underlying molecular mechanisms and prognostic factors of RNA binding protein in colorectal cancer: a study based on multiple online databases

Xue

et al. 2021

Cancer Cell Int

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Background RNA binding protein (RBP) is an active factor involved in the occurrence and development of colorectal cancer (CRC). Therefore, the potential mechanism of RBP in CRC needs to be clarified by dry-lab analyses or wet-lab experiments. Methods The differential RBP gene obtained from the GEPIA 2 (Gene Expression Profiling Interactive Analysis 2) were performed functional enrichment analysis. Then, the alternative splicing (AS) events related to survival were acquired by univariate regression analysis, and the correlation between RBP and AS was analyzed by R software. The online databases were conducted to analyze the mutation and methylation of RBPs in CRC. Moreover, 5 key RBP signatures were obtained through univariate and multivariate Cox regression analysis and established as RBP prognosis model. Subsequently, the above model was verified through another randomized group of TCGA CRC cohorts. Finally, multiple online databases and qRT-PCR analysis were carried to further confirm the expression of the above 5 RBP signatures in CRC. Results Through a comprehensive bioinformatics analysis, it was revealed that RBPs had genetic and epigenetic changes in CRC. We obtained 300 differentially expressed RBPs in CRC samples. The functional analysis suggested that they mainly participated in spliceosome. Then, a regulatory network for RBP was established to participate in AS and DDX39B was detected to act as a potentially essential factor in the regulation of AS in CRC. Our analysis discovered that 11 differentially expressed RBPs with a mutation frequency higher than 5%. Furthermore, we found that 10 differentially expressed RBPs had methylation sites related to the prognosis of CRC, and a prognostic model was constructed by the 5 RBP signatures. In another randomized group of TCGA CRC cohorts, the prognostic performance of the 5 RBP signatures was verified. Conclusion The potential mechanisms that regulate the aberrant expression of RBPs in the development of CRC was explored, a network that regulated AS was established, and the RBP-related prognosis model was constructed and verified, which could improve the individualized prognosis prediction of CRC.

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“…Most notably, upregulated LINC00941 could in turn activate the expression of its nearby gene CAPRIN2 , whereas chromatin organizer CTCF was required for this LINC00941 -induced CAPRIN2 overexpression. As CTCF has been shown to mediate the looping of the chromatin fragment between LINC00941 and CAPRIN2 genes through its binding sites being enriched within this region, these results suggested that LINC00941 might induce CAPRIN2 expression through CTCF-mediated DNA looping [ 39 ] ( Figure 1 b).…”

Section: Mechanism Of Oral Cancer-associated Lncrnas In Tumorigenesismentioning

confidence: 99%

“…This study not only supports the involvement of lncRNAs in Wnt signaling regulation, but also reveals the potential therapeutic value of CAFs. Meanwhile, the aforementioned lncRNA LINC00941 could provoke canonical Wnt signaling via upregulating the expression of Caprin-2 [ 39 ], a positive regulator of Wnt signaling, by promoting GSK3-depedent LRP5/6 phosphorylation ( Figure 2 a), and thereby promoting OSCC progression [ 141 ].…”

Section: Lncrnas Involved In Crucial Signaling Pathwaysmentioning

confidence: 99%

Long Non-Coding RNAs as Functional Codes for Oral Cancer: Translational Potential, Progress and Promises

Lei

Kung

Shih

2021

IJMS

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Oral cancer is one of the leading malignant tumors worldwide. Despite the advent of multidisciplinary approaches, the overall prognosis of patients with oral cancer is poor, mainly due to late diagnosis. There is an urgent need to develop valid biomarkers for early detection and effective therapies. Long non-coding RNAs (lncRNAs) are recognized as key elements of gene regulation, with pivotal roles in various physiological and pathological processes, including cancer. Over the past few years, an exponentially growing number of lncRNAs have been identified and linked to tumorigenesis and prognosis outcomes in oral cancer, illustrating their emerging roles in oral cancer progression and the associated signaling pathways. Herein, we aim to summarize the most recent advances made concerning oral cancer-associated lncRNA, and their expression, involvement, and potential clinical impact, reported to date, with a specific focus on the lncRNA-mediated molecular regulation in oncogenic signaling cascades and oral malignant progression, while exploring their potential, and challenges, for clinical applications as biomarkers or therapeutic targets for oral cancer.

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LINC00941 promotes oral squamous cell carcinoma progression via activating CAPRIN2 and canonical WNT/β‐catenin signaling pathway

Cited by 32 publications

References 45 publications

Novel lncRNA LINC00941 Promotes Proliferation and Invasion of Colon Cancer Through Activation of MYC

Novel lncRNA LINC00941 Promotes Proliferation and Invasion of Colon Cancer Through Activation of MYC

The underlying molecular mechanisms and prognostic factors of RNA binding protein in colorectal cancer: a study based on multiple online databases

Long Non-Coding RNAs as Functional Codes for Oral Cancer: Translational Potential, Progress and Promises

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