Linalool preferentially induces robust apoptosis of a variety of leukemia cells via upregulating p53 and cyclin-dependent kinase inhibitors

Gu, Ying; Zhang, Ting; Qiu, Xi; Zhang, Xuzhao; Gan, Xu; Fang, Yongming; Xu, Xiaohua; Xu, Rongzhen

doi:10.1016/j.tox.2009.11.013

Cited by 79 publications

(68 citation statements)

References 23 publications

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“…However, the molecular mechanism, which underlies this process, is still unclear. Results of some studies are consistent with the hypothesis that some essential oil components in cancer cellular lines act on cell cycle and apoptosis (Carnesecchi et al, 2001;Gu et al, 2010). Dudai and coworkers (2005) suggested that citral displays its proapoptotic activity through a direct procaspase 3 activation in human and mouse leukaemic cell lines.…”

Section: Introductionsupporting

confidence: 69%

Active caspase-3 detection to evaluate apoptosis induced by Verbena officinalis essential oil and citral in chronic lymphocytic leukaemia cells

Martino¹,

Arena²,

Minervini³

et al. 2011

Rev. bras. farmacogn.

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Verbena officinalis L., Verbenaceae, commonly known as vervain, is a plant widely used in medicine. Despite of its widespread use in different traditional practices, the mechanisms of pharmacological actions of the plant and its volatile oil are still unclear. We evaluated the pro-apoptotic activity of V. officinalis essential oil and of its main component, citral, on lymphocytes collected from ten patients with chronic lymphocytic leukaemia (CLL), a disease in which a faulty apoptotic mechanism is still retained one of the primary pathogenic events, by adding to treated mononuclear cells, annexin-V, propidium iodide, and CD19. Apoptosis was also evaluated using anti-active-caspase-3 monoclonal antibody after permeabilization of the cells. Both V. officinalis essential oil and citral were found able to induce apoptosis in CLL cells and to activate caspase-3, which is considered the way by means they active apoptosis in B neoplastic cells. This data further support evidences that indicate natural compounds as possible lead structure to develop new therapeutic agents for CLL.

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Section: Introductionsupporting

confidence: 69%

Active caspase-3 detection to evaluate apoptosis induced by Verbena officinalis essential oil and citral in chronic lymphocytic leukaemia cells

Martino¹,

Arena²,

Minervini³

et al. 2011

Rev. bras. farmacogn.

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“…15) It induces apoptosis in human leukemia cells. 16) Our previous study indicated that KEO mainly y To whom correspondence should be addressed. Tel/Fax: +81-172-39-3790; E-mail: hayatosp@cc.hirosaki-u.ac.jp Abbreviations: LPS, lipopolysaccharide; NO, nitric oxide; IL-6, interleukin-6; TNF-, tumor necrosis factor-; NO, inducible NO synthase; COX-2, cyclooxygenase-2; NF-B, nuclear factor-B; PPAR , peroxisome proliferator-activated receptor containing linalool showed antitumor activity against several human tumor cell lines.…”

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confidence: 99%

Kuromoji (Lindera umbellata) Essential Oil Inhibits LPS-Induced Inflammation in RAW 264.7 Cells

Maeda

Yamazaki

Katagata

2013

Bioscience, Biotechnology, and Biochemistry

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Kuromoji (Lindera umbellata) essential oil (KEO) has long been used in Japan as a traditional medicine. It contains linalool (C 10 H 18 O), a naturally occurring small terpenoid. For this study, we investigated the antiinflammatory effect of KEO in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Mouse macrophage-like RAW 264.7 cells were stimulated with LPS. Then they were treated with 25 or 50 g/mL of KEO for 24 h. KEO suppressed LPS-induced pro-inflammatory cytokine production such as that of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-(TNF-) in a dose-dependent manner. In addition, inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression and protein levels were suppressed by treatment with KEO cells. In addition, by treatment with 25 or 50 g/mL of linalool showed the same anti-inflammatory effect. The results suggest that KEO and linalool can be regarded as a natural resource for use in anti-inflammatory therapeutic products.

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“…The fragmented DNA content increased in cells treated with KEO and linalool for 48 h. This result suggests that KEO has leukemia cell apoptosis-inducing activity. Gu et al observed that linalool arrested growth arrest and caused apoptosis of various human leukemia cells, but noted that it spared normal hematopoietic cells (11). Linalool is the predominant component of KEO (65.78%).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, several recent reports have revealed that linalool reverses doxorubicin resistance in human breast adenocarcinoma cells (8) and exhibits antiproliferative activity against certain solid tumor cells, such as melanoma and renal cell adenocarcinoma cells (9), and HepG2 (10). Particularly, linalool induces apoptosis in human leukemia cells without affecting normal hematopoietic cell growth (11). These terpenoid components become ligands in nuclear receptors, regulate expression of various genes and ameliorate conditions related to these diseases (12).…”

Section: Introductionmentioning

confidence: 99%

Kuromoji (Lindera umbellata) essential oil-induced apoptosis and differentiation in human leukemia HL-60 cells

Maeda

Yamazaki

Katagata

2011

Experimental and Therapeutic Medicine

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Abstract. Essential oils diluted from certain plants have been shown to have antitumor activity against several human tumor cell lines. Kuromoji (Lindera umbellata) essential oil (KEO) has long been used in Japan as a traditional medicine. KEO and its major chemical constituent, linalool, were investigated in this study for their ability to induce apoptosis and differentiation in human leukemia HL-60 cells. HL-60 cells were treated with 5 or 50 µg/ml KEO or linalool for 24 or 48 h. Then, cell proliferation and apoptosis induction were estimated. In addition, HL-60 cells are known to differentiate into granulocyte or monocytes by a variety of compounds. Therefore, the effect of KEO or linalool on differentiation of HL-60 cells was assessed by Giemsa stain and a nitroblue tetrazolium reduction assay. Cells treated with KEO or linalool for 48 h showed significantly suppressed cell proliferation, with induced apoptosis. Moreover, KEO and linalool promoted cell differentiation. Treatment with KEO cells at the same dose as linalool showed an almost identical effect on HL-60 cells. These results suggest that KEO and linalool have efficacy as anticancer therapeutic products.

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Linalool preferentially induces robust apoptosis of a variety of leukemia cells via upregulating p53 and cyclin-dependent kinase inhibitors

Cited by 79 publications

References 23 publications

Active caspase-3 detection to evaluate apoptosis induced by Verbena officinalis essential oil and citral in chronic lymphocytic leukaemia cells

Active caspase-3 detection to evaluate apoptosis induced by Verbena officinalis essential oil and citral in chronic lymphocytic leukaemia cells

Kuromoji (Lindera umbellata) Essential Oil Inhibits LPS-Induced Inflammation in RAW 264.7 Cells

Kuromoji (Lindera umbellata) essential oil-induced apoptosis and differentiation in human leukemia HL-60 cells

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