Linalool decreases HepG2 viability by inhibiting mitochondrial complexes I and II, increasing reactive oxygen species and decreasing ATP and GSH levels

Usta, Jinan; Kreydiyyeh, Sawsan Ibrahim; Knio, Khouzama; Barnabe, Pascale; Bou-Moughlabay, Yolla; Dagher, Shawky M.

doi:10.1016/j.cbi.2009.02.012

Cited by 84 publications

(58 citation statements)

References 49 publications

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“…Using the MTT test, Usta et al (2009) also noted a decrease in the viability of HepG2 cells when exposed to 100 µM geraniol. When the same concentration was used to myrcene, monoterpene analogous to geraniol, the cell viability was reduced to 26% in HepG2.…”

Section: Discussionmentioning

confidence: 94%

Research Article Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).

et al. 2017

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ABSTRACT. Geraniol is an acyclic monoterpene alcohol present in the essential oil of many aromatic plants and is one of the most frequently used molecules by the flavor and fragrance industries. The literature also reports its therapeutic potential, highlighting itself especially as a likely molecule for the development of drugs against cancer. In view of these considerations, this study was designed to evaluate the cytotoxic and genotoxic potential of geraniol, in an in vitro protocol, using two types of human cells: one without the ability to metabolize (peripheral blood mononuclear cells -PBMC), and the other with this capability (human hepatoma cell line -HepG2) through the comet assay and the micronucleus test. Four concentrations (10, 25, 50, and 100 µg/ mL) were selected for the genotoxic assessment for PBMC and three (1.25, 2.5, and 5 µg/mL) for HepG2 cells based on cytotoxicity tests (MTT assay). Results showed that geraniol did not present genotoxic or clastogenic/aneugenic effects on both cell types under the conditions studied. However, caution is advised in the use of this substance by humans, since a significant reduction in viability of HepG2 and a marked decrease in cell viability on normal PBMC were verified.

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Section: Discussionmentioning

confidence: 94%

Research Article Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).

et al. 2017

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“…However, these speculations do not exclude the additive effect of FaNES1 transgene product toxicity yet such as inhibitory effects of linalool on the respiratory chain in mitochondria (Usta et al, 2009) and antioxidants against reactive oxygen species (Pompella et al, 2003) that already has been reported.…”

Section: Discussionmentioning

confidence: 96%

“…Other biochemical groups present in HIPV are green leaf volatiles, benzenoids and sulphur-or nitrogen-containing compounds. In the past decade, molecular genetic tools have been used to investigate the signalling pathways leading to HIPV emission and their effect on the attraction of carnivorous arthropods (Halitschke and Baldwin, 2003;Kessler and Baldwin, 2004;Snoeren et al, 2009;Van Poecke and Dicke, 2004). In addition, molecular insight into the biosynthesis of HIPVs has allowed the genetic modification of the production of these volatile compounds.…”

Section: Introductionmentioning

confidence: 99%

“…In order to reduce the harmful effects on plant growth and development, the levels of specific intermediates of the pathway, particularly those at branch-point positions should be controlled (Manzano et al, 2004). Knowing that an increase in precursor supply can elevate the level of terpenoids in plants in the presence of the corresponding terpene synthase Chen et al, 2000;van Herpen et al, 2010), the undesirable phenotypes can possibly be diminished by over-expressing FPS1L to provide a larger pool of FPP in the mitochondria. As isopentenyl diphosphate (IPP, the substrate for FPS1L) might be also a rate limiting factor, overexpression of A. thaliana HMGR1S (3-hydroxy-3-methylglutaryl coenzyme A reductase 1 short isoform) that encodes an earlier step in the mevalonate pathway and has been suggested to be rate limiting (Chappell et al, 1991) could further reduce the growth retardation.…”

Section: Introductionmentioning

confidence: 99%

“…Over-expression of HMGR1S has already been shown to prevent necrosis in leaves of FPS1S (short isoform of FPS which is cytosolic) overexpression lines (Manzano et al, 2004) and premature senescence in detached leaves of FPS1L (long isoform of FPS, which is mitochondrial) overexpression lines under continuous illumination (Manzano et al, 2006). Furthermore, transient co-expression of tHMGR (truncated form of HMGR) and FPS with amorphadiene synthase has been shown to improve amorphadiene biosynthesis in Nicotiana benthamiana (van Herpen et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

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et al. 2013

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, Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum, Metabolic Engineering, http://dx.doi.org/10.1016/j. ymben.2012.10.002 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Linalool: a review on a key odorant molecule with valuable biological properties

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Hăncianu

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et al. 2014

Flavour & Fragrance J

250

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This paper reports on the occurrence, biosynthesis, metabolism, biological and toxicological profile, and assessment of the authenticity of linalool. The main biological properties of linalool – sedative, anxiolytic, analgesic, anticonvulsant, anti‐inflammatory, local anaesthetic – are discussed in terms of the molecule's chirality influence, the mechanisms of activity and type of study (in vitro, in vivo, clinical studies). Also, there is a discussion of the recent data on the skin sensitizing potential of linalool based on numerous scientific studies which have been performed in the last few years. Comments of the authenticity assessment of linalool are made considering the limitations imposed by the chemical structure, vegetal matrix or processing methods, but also from the perspective of the powerful and sophisticated analytical techniques available today (GC‐C‐IRMS, enantio‐MDGC coupled to GC‐C‐IRMS, SNIF‐NMR). Copyright © 2014 John Wiley & Sons, Ltd.

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Linalool decreases HepG2 viability by inhibiting mitochondrial complexes I and II, increasing reactive oxygen species and decreasing ATP and GSH levels

Cited by 84 publications

References 49 publications

Research Article Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).

Research Article Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2).

Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

Linalool: a review on a key odorant molecule with valuable biological properties

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