Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

Abstract

, Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum, Metabolic Engineering, http://dx.doi.org/10.1016/j. ymben.2012.10.002 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Unraveling molecular mechanisms underlying plant defense in response to dual insect attack : studying density-dependent effects

“…Furthermore, the combination of both JA-induced volatiles and MeSA is important for the attraction of P. persimilis predatory mites towards Lima bean plants . In line with Houshyani et al (2013) (Houshyani et al, 2013) and has been found in P. xylostella-induced volatile blends from A. thaliana ; Supplementary materials Table S1). Other volatile compounds induced most strongly by feeding of P. xylostella caterpillars were β-mycrene and TMTT and, therefore, may contribute to the attraction of D. semiclausum parasitoids (Supplementary materials Table S1).…”

“…The microarray data indicated that the linalool synthase TPS10 gene was differentially expressed in response to feeding of both caterpillars and aphids at high density after 24 h compared to undamaged control plants, but not in response to dual insect infestation at low density (Chapter 6). Linalool has been reported to be an important attractant for D. semiclausum parasitoids (Houshyani et al, 2013) and induced biosynthesis of linalool could have influenced D. semiclausum preference for plants dually infested by caterpillars and aphids at high density. Results of Chapter 5 indicate that D. rapae parasitoids prefer volatiles emitted by dual-infested plants over plants infested by B. brassicae aphids alone, which is noteworthy.…”

Assessment of pleiotropic transcriptome perturbations in Arabidopsis engineered for indirect insect defence

“…Transformation of Col-3 plants used in this study was done by the insertion of 2 (in COX+) or 3 (in COX++) constructs, each containing the gene of interest ( COX-FaNes1 , FPS1L or HMGR1S ) (Figure 1a) and a selection-marker gene [17]. Quantitative RT-PCR showed de novo FaNES1 expression in the transgenic lines.…”

“…Quantitative RT-PCR showed de novo FaNES1 expression in the transgenic lines. FPS1L expression was at least 64-fold higher in COX + and COX++ and expression of HMGR1S was at least16-fold higher in COX++ lines than in Col-3 (the wild type) [17]. …”

“…Both represent pleiotropic and rate limiting enzymes [15,16]. Both lines emit the volatile compound, ( E )-nerolidol in the headspace and are similarly efficient in attraction of the endolarval parasitoid of Plutella xylostella (diamondback moth), Diadegma semiclausum [17]. The use of different strategies to generate the same trait can help to classify potential changes in the transcriptome into changes that are specifically associated with the novel trait(s) and changes that are non-specific.…”