LIN28 Expression in Rat Spinal Cord After Injury

Abstract

LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we performed an acute spinal cord contusion injury (SCI) model in adult rats and investigated the dynamic changes of LIN28 expression in spinal cord. Western blot and immunohistochemistry analysis revealed that LIN28 was present in normal spinal cord. It gradually increased, reached a peak at 3 day, and then nearly declined to the basal level at 14 days after SCI. Double immunofluorescence staining showed that LIN28 immunoreactivity was found in neurons, astrocytes and a handful of microglia. Interestingly, LIN28 expression was increased predominantly in astrocytes but not in neurons. Moreover, the colocalization of LIN28 and proliferating cell nuclear antigen was detected after injury. Western blot showed that LIN28 participated in lipopolysaccharide (LPS) induced astrocytes inflammatory responses by NF-κB signaling pathway. These results suggested that LIN28 may be involved in the pathologic process of SCI, and further research is needed to have a good understanding of its function and mechanism.

Multi-Level Regulatory Interactions between NF-κB and the Pluripotency Factor Lin28

“…The tissue and cellular expression profiles for Lin28a and Lin28b in mammals remain somewhat enigmatic, with low or undetectable levels reported in adults for most regions, with the exception of the testes, in atlas sources for mouse and human (e.g., ). This stands in possible discrepancy to accumulating reports of Lin28a and Lin28b presence and function in adult organisms or differentiated cells and tissues [ 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ], despite clear downregulation in levels from early development. Ineffective detection is not unfamiliar amongst low abundance proteins and transcripts which may not be readily quantified or documented in databases and could be contributed to by a failure to capture signal-dependent upregulation.…”

Drinking desalinated seawater for a long time induces anomalies in the development of new-born albino rats

“…Oxidative stress leads to neuronal damage in several brain regions and hepatic and renal tissue damage (Allam et al, 2012). For example, neuronal loss in the cerebrum impairs an animal’s memory (Abu-Taweel et al, 2012), neuronal loss in the cerebellum can have an effect on balance and coordination (Allam et al, 2011), and neuronal loss in the medulla oblongata and spinal cord can affect the physical activity of mice (Yue et al, 2014).…”

Protective Effect of Parsley Juice (Petroselinum crispum, Apiaceae) against Cadmium Deleterious Changes in the Developed Albino Mice Newborns (Mus musculus) Brain

“…The oxidative stress leads to neuronal damage in several brain regions [ 40 , 49 , 55 ]. For example, neuronal loss in cerebrum impairs animal's memory [ 38 ], neuronal loss in cerebellum can have effect on balance and coordination [ 40 ], and neuronal loss in medulla oblongata and spinal cord can affect physical activity of mice [ 56 ].…”