LiMYB108 is involved in floral monoterpene biosynthesis induced by light intensity in Lilium ‘Siberia’

Yang, Yunyao; Zhang, Xi; Ming-Zheng, Han; Hu, Zenghui; Wu, Jing; Ma, Nan; Leng, Pei; Zhou, Xiaofeng

doi:10.1007/s00299-023-02995-x

Cited by 11 publications

(1 citation statement)

References 40 publications

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“…MYC2 , a basic helix-loop-helix ( bHLH ) TF in Arabidopsis , directly regulates the formation of floral volatile terpenes [ 57 ]. Moreover, scent-related TFs, such as MYB , WRKY , bHLH , bZIP , and AP2/ERF , have regulatory effects on key structural genes for floral scent production [ 58 , 59 , 60 , 61 , 62 ]. This study identified 2123 TFs, including 1133 differentially expressed TFs.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses Reveal the Aroma Terpeniods Biosynthesis Pathways of Primula forbesii Franch. and the Functional Characterization of the PfDXS2 Gene

Yin

Yang

et al. 2023

IJMS

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Primula forbesii Franch. is a unique biennial herb with a strong floral fragrance, making it an excellent material for studying the aroma characteristics of the genus Primula. The floral scent is an important ornamental trait that facilitates fertilization. However, the molecular mechanism regulating the floral scent in Primula is unknown. In order to better understand the biological mechanisms of floral scents in this species, this study used RNA sequencing analysis to discuss the first transcriptome sequence of four flowering stages of P. forbesii, which generated 12 P. forbesii cDNA libraries with 79.64 Gb of clean data that formed 51,849 unigenes. Moreover, 53.26% of the unigenes were annotated using public databases. P. forbesii contained 44 candidate genes covering all known enzymatic steps for the biosynthesis of volatile terpenes, the major contributor to the flower’s scent. Finally, 1-deoxy-d-xylulose 5-phosphate synthase gene of P. forbesii (PfDXS2, MK370094), the first key enzyme gene in the 2-c-methyl-d-erythritol 4-phosphate (MEP) pathway of terpenoids, was cloned and functionally verified using virus-induced gene silencing (VIGs). The results showed that PfDXS2-silencing significantly reduced the relative concentrations of main volatile terpenes. This report is the first to present molecular data related to aroma metabolites biosynthesis pathways and the functional characterization of any P. forbesii gene. The data on RNA sequencing provide comprehensive information for further analysis of other plants of the genus Primula.

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