Positive regulatory role of R2R3 MYBs in terpene biosynthesis in Lilium ‘Siberia’

Guo, Yanhong; Guo, Ziyu; Zhong, Jian; Liang, Yilin; Feng, Yuan; Zhang, Peng; Zhang, Qixiang; Sun, Ming

doi:10.1016/j.hpj.2023.05.004

Cited by 12 publications

(6 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biosynthesis of floral aroma is both complex and coordinately controlled, and the regulation of gene transcription by TFs is a key aspect of control. 38,39 In the present study, the integrated approach of volatilomics and transcriptomics revealed that the expression of many TFs was significantly correlated with the release of key odorants of jasmine (Figure 2), and many TFs were identified as key hub genes, including four MYB-relateds, two HB-others, one DBB, one C3H, one C2H2, one Trihelix, one NAC, and one SBP (Figure 3D). These TFs have been reported to regulate the formation of aroma compounds by controlling the expression of key genes in aroma synthesis pathways.…”

Section: ■ Discussionmentioning

confidence: 53%

Multiomics Analysis Reveals the Involvement of JsLHY in Controlling Aroma Production in Jasmine Flowers

Zhou,

Tian,

Wen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

The Jasminum sambac flower is famous for its rich fragrance. However, our knowledge of the regulatory network for its aroma formation remains largely unknown and therefore needs further study. To this end, an integrated analysis of the volatilomics and transcriptomics of jasmine flowers at different flowering stages was performed. The results revealed many candidate transcription factors (TFs) may be involved in regulating the aroma formation of jasmine, among which the MYB-related TF LATE ELONGATED HYPOCOTYL (JsLHY) was identified as a hub gene. Using the DNA affinity purification sequencing method, dualluciferase reporter, and yeast one-hybrid assays, we demonstrate that JsLHY can bind the gene promoter regions of six aroma-related structural genes (JsBEAT1, JsTPS34, JsCNL6, JsBPBT, JsAAAT5, and Js4CL7) and directly promote their expression. In addition, suppressing JsLHY expression decreased both the expression of JsLHY-bound genes and the content of related VOCs. The present study reveals how JsLHY participates in jasmine aroma formation.

show abstract

Section: ■ Discussionmentioning

confidence: 53%

Multiomics Analysis Reveals the Involvement of JsLHY in Controlling Aroma Production in Jasmine Flowers

Zhou,

Tian,

Wen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…The main families of TFs involved in terpene biosynthesis in plants are bHLH [ 47 , 48 ], AP2/ERF [ 49 ], NAC [ 50 ], WRKY [ 51 ], MYB [ 52 ], and bZIP [ 53 ]. For example, terpene biosynthesis in Litsea cubeba was regulated by LcbHLH78 and LcTPS42 , and the overexpression of LcbHLH78 significantly increased the content of α-pinene, linalool, and geraniol [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry Metabolites and Transcriptome Profiling Reveal Molecular Mechanisms and Differences in Terpene Biosynthesis in Two Torrya grandis Cultivars during Postharvest Ripening

Zhang,

Gao,

Tao

et al. 2024

IJMS

View full text Add to dashboard Cite

Terpene aroma compounds are key quality attributes of postharvest Torreya grandis nuts, contributing to their commercial value. However, terpene biosynthesis and regulatory networks in different T. grandis cvs. are still poorly understood. Here, chief cvs. ‘Xi Fei’ and ‘Xiangya Fei’ were investigated for their differences in terpene biosynthesis and gene expression levels during postharvest ripening using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC-MS) and transcriptomic datasets. A total of 28 and 22 aroma compounds were identified in ‘Xi Fei’ and ‘Xiangya Fei’, respectively. Interestingly, differences in aroma composition between the two cvs. were mostly attributed to D-limonene and α-pinene levels as key determinants in Torreya nuts’ flavor. Further, transcriptome profiling, correlation analysis, and RT-qPCR annotated two novel genes, TgTPS1 in ‘Xi Fei’ and TgTPS2 in ‘Xiangya Fei’, involved in terpene biosynthesis. In addition, six transcription factors (TFs) with comparable expression patterns to TgTPS1 and four TFs to TgTPS2 were identified via correlation analysis of a volatile and transcriptome dataset to be involved in terpene biosynthesis. Our study provides novel insight into terpene biosynthesis and its regulation at the molecular level in T. grandis nut and presents a valuable reference for metabolic engineering and aroma improvement in this less explored nut.

show abstract

“…Overexpression of LiMYB1 (S4), LiMYB305 (S19), and LiMYB330 (S4) in flowers increases the release of some major monoterpenes, such as linalool and ocimene, while the expression of LiTPS2 is enhanced. In addition, LiMYB1 (S4) from the same subfamily can interact with LiMYB308 (S4) and LiMYB330 (S4), indicating their synergistic effect in regulating terpene biosynthesis [89].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of MYB Transcription Factors and Their Function on Floral Volatile Compounds Biosynthesis in Antirrhinum majus L.

Song,

Shi,

Kong

et al. 2024

Horticulturae

View full text Add to dashboard Cite

The v-MYB avivan myoblastsis virus oncogene homolog (MYB) family is the largest gene family of the transcription factor in plants, involved in plant growth and development, secondary metabolism and resistance to biotic/abiotic stress. Antirrhinum majus (snapdragon) is an ideal material for studying ornamental traits. Nevertheless, there has been no systematic investigation into the AmMYB family of snapdragons. In this study, we identified a total of 162 members of the AmMYB gene family in snapdragons. Gene structure analysis showed that the AmMYB family within the same subgroup had a similar structure and motifs. Analysis of gene duplication events revealed that the amplification of the AmMYB family was driven by whole-genome duplication (WGD) and dispersed duplication. The analysis of cis-acting elements in the promoter region of AmMYB genes reveals a collaborative involvement of light-responsive growth and development elements, stress resistance elements, and hormone-responsive elements jointly participating in the regulation of the AmMYB gene. Collinearity analysis demonstrates significant functional distinctions between AmMYB and monocotyledonous plants. The classification of AmMYB members results in 3 main subgroups with 36 smaller subgroups. All AmMYB genes are distributed across all eight chromosomes, with no apparent correlation between subfamily distribution and chromosome length. Through phylogenetic analysis and RNA-seq analysis, we have identified 9 R2R3-MYB genes that potentially play a role in the regulation of floral volatile organic compounds (FVOCs) biosynthesis. Their expression patterns were verified by qRT-PCR experiments. This study establishes a robust foundation for further investigations into the functionality of AmMYB genes and their molecular mechanisms underlying FVOC biosynthesis in snapdragons.

show abstract

Positive regulatory role of R2R3 MYBs in terpene biosynthesis in Lilium ‘Siberia’

Cited by 12 publications

References 52 publications

Multiomics Analysis Reveals the Involvement of JsLHY in Controlling Aroma Production in Jasmine Flowers

Multiomics Analysis Reveals the Involvement of JsLHY in Controlling Aroma Production in Jasmine Flowers

Gas Chromatography-Mass Spectrometry Metabolites and Transcriptome Profiling Reveal Molecular Mechanisms and Differences in Terpene Biosynthesis in Two Torrya grandis Cultivars during Postharvest Ripening

Genome-Wide Identification of MYB Transcription Factors and Their Function on Floral Volatile Compounds Biosynthesis in Antirrhinum majus L.

Contact Info

Product

Resources

About